Objective To investigate the correlation between lipid accumulation index (LAP),visceral fat index (VFI) and insulin resistance (IR) in healthy check-up population.Methods A total of 9121 subjects who underwent physical examination in the Health Management Center of the First Affiliated Hospital of Ar-my Medical University from October 2016 to June 2023 were selected as the study subjects,including 5988 males and 3133 females.The age,waist circumference (WC),body mass index (BMI),LAP,VFI,blood lipid, blood glucose and homeostasis model assessment of insulin resistance (HOMA-IR) were compared between different genders.Obesity was assessed according to BMI.LAP,VFI and HOMA-IR were compared between obese (BMI≥28 kg/m2) and non-obese patients (BMI<28 kg/m2).Pearson correlation analysis was used to analyze the relationship between LAP,VFI and HOMA-IR in all healthy subjects.Multiple logistic regression analysis was used to analyze the independent influencing factors of HOMA-IR in all healthy subjects. Results The age and high-density lipoprotein cholesterol (HDL-C) of male healthy subjects were significant-ly lower than those of female,while WC,BMI,triglyceride (TG),total cholesterol (TC),low-density lipopro-tein cholesterol (LDL-C),LAP,VFI,fasting blood glucose (FBG),fasting insulin (FINS),glycosylated hemo-globin (HbA1c) and HOMA-IR were significantly higher than those of female (P<0.05).There were 1733 cases of obesity in all healthy subjects,accounting for 19％.The LAP,VFI and HOMA-IR of obese people were significantly higher than those of non-obese people (P<0.05).Pearson correlation analysis showed that HOMA-IR was positively correlated with WC,BMI,TG,TC,LDL-C,LAP and VFI,and negatively correlated with HDL-C (P<0.05).The correlation coefficient r value of LAP was the highest,reaching 0.367,followed by WC,BMI,TG and VFI.Multivariate logistic correlation analysis showed that BMI,LAP and VFI were in-dependent influencing factors of HOMA-IR in all healthy subjects (P<0.05).Conclusion Obesity should not be ignored in health check-up population.LAP and VFI are associated with IR,especially LAP,which can reliably evaluate body fat accumulation and blood glucose metabolism.