The distribution and types of fibronectin (Fn) on vessel wall and platelt surface in human being and rat were studied with rabbit anti-human and rabbit anti-rat Fn antisera and monoclonal antibody against human Fn. It was found that the Fn of mainly cellulartype is chiefly distributed within the layer of subendothelium and near the luminal surface of aorta. In connective tissue of the adventitia there was a large amount of Fn of the plasma and cellular types. The distribution of Fn in the wall of the middle-sized arteries was similar to that in the aortic wall. The Fn of the cellular type in the human aortic subendothelium was more abundant than that in the rat. There were only little amount of Fn on the surface of resting platelet. Nevertheless, when they were activated by thrombin, the amount of Fn on their surface increased, chiefly of the plasma type. The r sult of this work seems to have practical significance in the investigation of the mechanism of platelet adhesion on vessel subendothelium matrix.

High-throughput metabolomics have developed very rapidly in recent years and been widely used in medicinal plants research .At present ,high-throughput metabolomics mainly applied in the following areas ,quality control of medicinal plants by fingerprints ,metabolites difference comparison before and after genetic engineering ,monitoring metabolites change in different environment and gene function study .High-throughput metabolomics have a great future ,but still have some challen-ges ,such as the requirements for more sophisticated equipment and complexity of data integration .With the advancement of science and technology ,high-throughput metabolomics will be an irreplaceable tool for the research of medicinal plants .

Baphicacanthus cusia ,widely distributed in the Southwest ,Southern and Eastern regions of China ,is an im-portant medicinal plant of Acanthaceae family .Indigo made from stem and leaf of Baphicacanthus cusia in Fujian has the best quality in China ,and is known as “Jian Indigo naturalis” ,which is the genuine medicinal of Fujian Province .The rhizoma of Baphicacanthus cusia could be used as medicine ,called“Nanbanlangen” ,which together with indigo were included in the“Chi-nese Pharmacopoeia” .Indigo and its original plant both contain indirubin ,which has anti-cancer activity .Indirubin is an active ingredient of Huangdai Pian and Danggui Longhui Wan ,two kinds of traditional Chinese medicine ,which have been successful-ly used in the treatment of malignancies such as chronic myelogenous leukemia .The international advances in the biological characteristics ,genetic diversity ,cultivation technology and molecular biology of Baphicacanthus cusia germplasm resources were summarized .The main problems in Baphicacanthus cusia germplasm resources research are indicated ,which could pro-vide references for the further study and application of Baphicacanthus cusia germplasm resources .

Objective: To investigate whether elevated levels of C-reactive protein (CRP) and neutrophil (NE) in the blood is associated with an increased risk of lung cancer incidence. Methods: From 2006 to 2007, all employees and retirees from Kailuan (Group) Limited liability Corporation were included in this Kailuan Cohort study. The last follow-up date was December 2015. Data on new cases of lung cancer were collected, and multivariable Cox proportional hazards regression models were used to the relationship between baseline CRP and NE at baseline and risk of lung cancer. Results: A total of 92 735 participants were enrolled in this study. During the follow-up, 850 new cases of lung cancer were identified. All subjects were divided into four groups according to the combination level of CRP and NE at baseline: CRP≤3 mg/L and NE≤4×10⁹/L(Group A), CRP≤3 mg/L and NE>4×10⁹/L(Group B), CRP>3 mg/L and NE≤4×10⁹/L(Group C), CRP>3 mg/L and NE>4×10⁹/L(Group D). The cumulative incidence of lung cancer were 950/100 000, 1 030/100 000, 1 081/100 000 and 1 596/100 000 in these four groups, respectively (P<0.001). Multivariate Cox proportional risk model showed that participants from Group D had an significantly increased 72% risks of lung cancer when compared to Group A (95% CI: 1.40~2.12, P<0.001). Stratified analyses gender showed that males in Group D had higher risk of lung cancer when compared with participants in Group A (HR=1.73, 95% CI: 1.40~2.15, P<0.001). Conclusion Elevated levels of CRP and NE might increase the risk of lung cancer.