Objective: To investigate the influence of hepatitis B virus X gene (HBx) on apoptosis of hepatic cells mediated by Fas in HePG2 cells. Methods: HBx eukaryotic vector pcDNA3.1(+)-X was transfected into HEPG2 cells with lipofectamine, and the null vector pcDNA3.1(+) and untransfected HEPG2 were used as normal controls. The cells were collected 72 h after transfection, and the expression of HBx mRNA and protein was determined using RT-PCR and Western blot, respectively. The mRNA expression of apoptosis-related genes Bcl-2 and Bax mRNA was also determined using RT-PCR. Cytotoxicity and apoptosis were evaluated using CCK-8 and flow cytometry, respectively, after HepG2-HBx and HepG2-3.1 cells were treated with stimulatory monoclonal antibody anti-Fas CH11. The t test was used for pairwise comparison. Results: The cell line HepG2-HBx was successfully established, as confirmed by RT-PCR and Western blot, and RT-PCR results showed that HepG2-HBx cells had significantly higher expression of Bcl-2 mRNA than HepG2-3.1 and HepG2 cells (P < 0.05), but had significantly lower expression of Bax mRNA than HepG2-3.1 and HepG2 cells (P < 0.05); CCK-8 and flow cytometry showed that anti-Fas CH11 had a lower cytotoxicity to HepG2-HBx cells and allowed for a lower apoptosis rate of HepG2-HBx cells compared with HepG2-3.1 and HepG2 cells. Conclusions HBx can inhibit apoptosis of hepatic cells mediated by the Fas pathway.

Objective:To investigate the factors that contribute to acute lung injury(ALI)following thoracoscopic surgery in elderly patients with esophageal cancer.Methods:This study retrospectively analyzed the clinical data of 300 elderly patients with esophageal cancer who were treated in the hospital between June 2018 and June 2022.The baseline data of the patients were recorded and they were divided into two groups: ALI group and non-ALI group based on the postoperative complication of ALI.The study compared the baseline data of the two groups and used Logistic regression analysis to identify the influencing factors of ALI after thoracoscopic surgery in elderly patients with esophageal cancer.Results:This study involving 300 elderly patients with esophageal cancer found that 35 had ALI while 265 did not.Multiple Logistic regression analysis revealed that age increase( OR=1.573, P=0.031), a large number of lymph nodes cleaned( OR=3.145, P=0.008), and long tracheal intubation time( OR=1.108, P=0.028)were identified as risk factors for ALI in elderly esophageal cancer patients after thoracoscopic surgery.Conversely, high albumin levels were found to be a protective factor( OR=0.434, P=0.035). Conclusions:The incidence of acute lung injury(ALI)following thoracoscopic surgery in elderly patients with esophageal cancer is significantly high.Several factors contribute to this risk, including patient age, the number of lymph nodes cleaned, albumin level, and tracheal intubation time.