1.Polyarteritis nodosa of the breast.
Chan Heun PARK ; Koo KANG ; Soo Tong PAI
Journal of the Korean Surgical Society 1991;41(4):544-548
No abstract available.
Breast*
;
Polyarteritis Nodosa*
2.A Case Report of Central Nervous System Toxicity following Accidental Injection of Tranexamic acid into Subarachnoid space.
Gill Soo LEW ; Seung Tak RYOO ; Heun Cheul SOUNG ; Jin Cheul JOO
Korean Journal of Anesthesiology 1993;26(6):1300-1305
Neurologic Sequelae after spinal anesthesia are extrenely rare, due in part to use of prepackaged and sterile kits and the small doses of local anesthectics employed. We have experienced 42 years old healthy male developed cental nervous system toxicity due to injection of wrong substance into subarachnoid space. And the patient recovered 3 days later with mild pulmonary edema and about 72 hour anterograde amnesia after symptomatic treatment.
Adult
;
Amnesia, Anterograde
;
Anesthesia, Spinal
;
Central Nervous System*
;
Humans
;
Male
;
Nervous System
;
Pulmonary Edema
;
Subarachnoid Space*
;
Tranexamic Acid*
3.Compound K, a Metabolite of Ginsenosides, Attenuates Collagen-induced Arthritis in Mice.
Yun Jong LEE ; Kye Yong SONG ; Eun Young LEE ; Heun Soo KANG ; Yeong Wook SONG
Journal of Rheumatic Diseases 2015;22(3):154-166
OBJECTIVE: Although several ginsenosides have been reported to have anti-arthritic activity, few in vivo studies of the anti-arthritic effects of compound K (CK), a major metabolite of ginsenosides, have been conducted. Therefore, we investigated the preventative and therapeutic effects of CK on collagen-induced arthritis (CIA). METHODS: CK was administered to CIA mice preventively and therapeutically and post-treatment bone microarchitectural characteristics, histopathological changes, and serum levels of anti-collagen antibodies, tumor necrosis factor-alpha, and interleukin (IL)-17 were investigated. We also examined cytokine production by type II collagen (CII)-stimulated splenocytes and mRNA expression of matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinase (TIMP)-1, receptor activator of nuclear factor-kappaB ligand (RANKL), and osteoprotegerin (OPG) in the joint tissues. RESULTS: CK reduced the severity of CIA preventively and therapeutically (all p<0.05). Additionally, CK dose-dependently decreased histopathological signs of arthritis and improved microarchitectural characteristics (all p<0.05) at 10 to 20 mg/kg/d in CIA mice. CK treatment significantly decreased the serum levels of anti-CII immunoglobulin G (p<0.01) and the secretion of interferon-gamma and IL-2 from stimulated splenocytes (all p<0.05). Furthermore, MMP-3/TIMP-1 and RANKL/OPG ratios were suppressed in CK treated mice (all p<0.01). CONCLUSION: CK attenuated CIA via suppression of the humoral immune response and modulation of joint-destructive mediators. These results suggest that CK has therapeutic potential in rheumatoid arthritis.
Animals
;
Antibodies, Neoplasm
;
Arthritis
;
Arthritis, Experimental*
;
Arthritis, Rheumatoid
;
Collagen Type II
;
Ginsenosides*
;
Immunity, Humoral
;
Immunoglobulin G
;
Interferon-gamma
;
Interleukin-2
;
Interleukins
;
Joints
;
Matrix Metalloproteinases
;
Mice*
;
Necrosis
;
Osteoprotegerin
;
Panax
;
RANK Ligand
;
RNA, Messenger
4.Antioxidant and antiobesity activities of oral treatment with ethanol extract from sprout of evening primrose (Oenothera laciniata) in high fat diet-induced obese mice
Chung Shil KWAK ; Mi Ju KIM ; Sun Gi KIM ; Sunyeong PARK ; In Gyu KIM ; Heun Soo KANG
Journal of Nutrition and Health 2019;52(6):529-539
PURPOSE: Sprouts of evening primrose (Oenothera laciniata, OL) were reported to have high contents of flavonoids and potent antioxidant activity. This study examined the antioxidant and antiobesity activities of OL sprouts to determine if they could be a natural health-beneficial resource preventing obesity and oxidative stress.METHODS: OL sprouts were extracted with 50% ethanol, evaporated, and lyophilized (OLE). The in vitro antioxidant activity of OLE was examined using four different tests. The antiobesity activity and in vivo antioxidant activity from OLE consumption were examined using high fat diet-induced obese (DIO) C57BL/6 mice.RESULTS: The IC₅₀ for the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging and superoxide dismutase (SOD)-like activities of OLE were 26.2 µg/mL and 327.6 µg/mL, respectively. OLE exhibited the ferric reducing antioxidant power (FRAP) activity of 56.7 µg ascorbic acid eq./mL at 100 µg/mL, and an increased glutathione level by 65.1% at 200 µg/mL compared to the control in the hUC-MSC stem cells. In an animal study, oral treatment with 50 mg or 100 mg of OLE/kg body weight for 14 weeks reduced the body weight gain, visceral fat content, fat cell size, blood leptin, and triglyceride levels, as well as the atherogenic index compared to the high fat diet control group (HFC) (p < 0.05). The blood malondialdehyde (MDA) level and the catalase and SOD-1 activities in adipose tissue were reduced significantly by the OLE treatment compared to HFC as well (p < 0.05). In epididymal adipose tissue, the OLE treatment reduced the mRNA expression of leptin, PPAR-γ and FAS significantly (p < 0.05) compared to HFC while it increased adiponectin expression (p < 0.05).CONCLUSION: OLE consumption has potent antioxidant and antiobesity activities via the suppression of oxidative stress and lipogenesis in DIO mice. Therefore, OLE could be a good candidate as a natural resource to develop functional food products that prevent obesity and oxidative stress.
Adipocytes
;
Adipokines
;
Adiponectin
;
Adipose Tissue
;
Animals
;
Ascorbic Acid
;
Body Weight
;
Catalase
;
Diet, High-Fat
;
Ethanol
;
Flavonoids
;
Functional Food
;
Glutathione
;
In Vitro Techniques
;
Intra-Abdominal Fat
;
Leptin
;
Lipogenesis
;
Malondialdehyde
;
Mice
;
Mice, Obese
;
Natural Resources
;
Obesity
;
Oenothera biennis
;
Oxidative Stress
;
RNA, Messenger
;
Stem Cells
;
Superoxide Dismutase
;
Triglycerides
5.Erratum: Antioxidant and antiobesity activities of oral treatment with ethanol extract from sprout of evening primrose (Oenothera laciniata) in high fat diet-induced obese mice
Chung Shil KWAK ; Mi-Ju KIM ; Sun Gi KIM ; Sunyeong PARK ; In Gyu KIM ; Heun Soo KANG
Journal of Nutrition and Health 2020;53(6):702-702
6.Dehydroepiandrosterone-dependent induction of peroxisomal proliferation can be reduced by aspartyl esterification without attenuation of inhibitory bone loss in ovariectomy animal model.
Chung Shil KWAK ; Chang Mo KANG ; Heun Soo KANG ; Kye Yong SONG ; Mee Sook LEE ; Sang Cheol SEONG ; Sang Chul PARK
Journal of Korean Medical Science 2000;15(5):533-541
The purpose of this study was to determine whether esterification of dehydroepiandrosterone with aspartate (DHEA-aspartate) could reduce peroxisomal proliferation induced by DHEA itself, without loss of antiosteoporotic activity. Female Sprague-Dawley rats were ovariectomized, then DHEA or DHEA-aspartate was administered intraperitoneally at 0.34 mmol/kg BW 3 times a week for 8 weeks. DHEA-aspartate treatment in ovariectomized rats significantly increased trabeculae area in tibia as much as DHEA treatment. Urinary Ca excretion was not significantly increased by DHEA or DHEA-aspartate treatment in ovariectomized rats, while it was significantly increased by ovariectomy. Osteocalcin concentration and alkaline phosphatase activity in serum and cross linked N-telopeptide type I collagen level in urine were not significantly different between DHEA-aspartate and DHEA treated groups. DHEA-aspartate treatment significantly reduced liver weight and hepatic palmitoyl-coA oxidase activity compared to DHEA treatment. DHEA-aspartate treatment maintained a nearly normal morphology of peroxisomes, while DHEA treatment increased the number and size of peroxisomes in the liver. According to these results, it is concluded that DHEA-aspartate ester has an inhibitory effect on bone loss in ovariectomized rats with a marked reduction of hepatomegaly and peroxisomal proliferation compared to DHEA.
Adjuvants, Immunologic/pharmacology*
;
Adjuvants, Immunologic/metabolism
;
Adjuvants, Immunologic/chemistry
;
Animal
;
Aspartic Acid/pharmacology*
;
Aspartic Acid/metabolism
;
Aspartic Acid/chemistry
;
Biological Markers
;
Calcium/urine
;
Calcium/blood
;
Disease Models, Animal
;
Esterification
;
Fatty Acid Desaturases/metabolism
;
Female
;
Injections, Intraperitoneal
;
Lipoproteins, HDL Cholesterol/blood
;
Lipoproteins, LDL Cholesterol/blood
;
Liver/enzymology
;
Liver/drug effects
;
Organ Weight
;
Osteoporosis/pathology
;
Osteoporosis/metabolism*
;
Osteoporosis/drug therapy*
;
Ovariectomy*
;
Peroxisomes/metabolism*
;
Prasterone/pharmacology*
;
Prasterone/metabolism
;
Prasterone/chemistry
;
Rats
;
Rats, Sprague-Dawley
;
Tibia/pathology
;
Tibia/metabolism
;
Triglycerides/blood
7.Characterization of Vancomycin-Resistant Enterococci Isolated from Stools and Their Acquisition of Vancomycin Resistance.
Hee Jeong KIM ; Heun Young KWON ; Kang Lim KIM ; Hyo Jin LEE ; Hyun Jung JO ; Soo Myung HWANG ; Kyung Soo CHANG
Journal of Bacteriology and Virology 2010;40(4):179-189
We have isolated 6 vancomycin resistant (VR) Enterococcus faecium and 5 VR-E. gallinarum. Vancomycin resistant enterococcus (VRE) isolates were resistant to multi-drugs, but susceptible to linezolid and quinupristin/dalfopristin. VRE isolates showed 10 VanA phenotypes and 1 VanB phenotype (E. gallinarum). However, all of them showed vanA genotype. vanA gene was detected on both genomic and plasmid DNA from all VRE isolates. Almost of VR-E. faecium had IS1216V which is worldwide type and almost of VR-E. gallinarum had IS1542 which is European type. IS1216V and IS1542 genes were not related with antibiotic types of VRE. Copy numbers of vanA were decreased in VRE with IS1216V or IS1542 but not in VRE with both ISs in broth without vancomycin. The copy numbers of vanA were significantly decreased in VanB phenotype of VRE with IS1542 in broth without vancomycin. Copy numbers of vanA were recovered in the presence of vancomycin. Growth time of reference E. faecium is faster than that of reference E. faecalis when cultured in the broth containing vancomycin. Reference strains cultured in the broth containing vancomycin showed intermediate resistance or resistance to antibiotics without acquisition of van genes. Naturally, multidrug-resistant E. faecium might be fast adapted in the presence of vancomycin compared to E. faecalis. Taken together, VanA phenotype E. gallinarum as well as E. feacium have been increasing in nosocomial infection and showed acquired inducible resistance. E. faecium and E. faecalis showed intermediate resistance in long exposure of vancomycin without acquisition of vanA.
Acetamides
;
Anti-Bacterial Agents
;
Coat Protein Complex I
;
Cross Infection
;
DNA
;
Enterococcus
;
Enterococcus faecium
;
Genotype
;
Oxazolidinones
;
Phenotype
;
Plasmids
;
Vancomycin
;
Vancomycin Resistance
;
Linezolid
8.A Case of P-ANCA Positive Necrotizing Glomerulonephritis with Eosinophilia.
Jang Yel SHIN ; Ea Wha KANG ; Dong Ryeol RYU ; Jung Sik SONG ; Won Ki LEE ; Yong Beom PARK ; Lucia KIM ; Heun Ju JUNG ; Soo Kon LEE
The Journal of the Korean Rheumatism Association 2000;7(1):83-89
Antineutrophil cytoplasmic antibodies (ANCAs) are now regarded as a serologic marker for pauci-immune crescentic necrotizing glomerulonephritis either in renal-limited form or in association with systemic vasculitis, such as Wegener? granulomatosis, microscopic polyarteritis, and Churg-Strauss syndrome. Two major ANCA antigens have been indentified: proteinase3, which produces a cytoplasmic staining pattern termed C-ANCA, and myeloperoxidase, which produces a perinuclear pattern termed P-ANCA on ethanol-fixed neutrophils by indirect immunofluorescence. In ANCA- associated diseases, eosinphilia in excess of 1.5X109/L has been proposed to be characteristic of Churg-Strauss syndrome and is rare in other forms of ANCA-associated systemic vasculitis and crescentic necrotizing glomerulonephritis. Recently, there were two cases of P-ANCA positive crescentic necrotizing glomerulonephritis with peripheral blood eosinophilia and extrarenal microscopic vasculitis without asthma or granulomas. We experienced a patient with P-ANCA positive pauci-immune necrotizing glomerulonephritis with few eosinophilic infiltration and eosinophilia. He improved with oral prednisolone along with combination of intravenous cyclophosphamide. So we report this case with the review of literature.
Antibodies, Antineutrophil Cytoplasmic*
;
Asthma
;
Churg-Strauss Syndrome
;
Cyclophosphamide
;
Cytoplasm
;
Eosinophilia*
;
Eosinophils
;
Fluorescent Antibody Technique, Indirect
;
Glomerulonephritis*
;
Granuloma
;
Humans
;
Neutrophils
;
Peroxidase
;
Prednisolone
;
Systemic Vasculitis
;
Vasculitis
9.The changes of salivary microorganism composition after therapeutic radiation for oral cancer patients.
Jong Ho LEE ; Myung Jin KIM ; Pill Hoon CHOUNG ; Jin Young CHOI ; Byoung Moo SEO ; Ro Heun SONG ; Kang Min AHN ; Jong Won KIM ; Il Woo NAM ; Soo Kyung KIM
Journal of the Korean Association of Oral and Maxillofacial Surgeons 2000;26(1):18-23
The changes of the microorganism composition after therapeutic radiation for oral cancer patients are not well known and the long-term follow-up data are not reported. To obtain basic data for understanding of pathogenesis and prevention and treatment of dental caries and mucositis occuring after radiation therapy, 7 of the oral cancer patients presented at the Seoul National University Oral & Maxillofacial Department between 1997 and 1998 whose treatment plan included radiation therapy were recruited to investigate the changes in bacterial composition(total aerobic count, candida, Staphylococci, lactobacilli, S. mutans, and S. salivarius (mitis, sanguis)) of the saliva before, during, and after radiation therapy. The basic data obtained from this study on identification and composition change of the bacteria in saliva of patients treated with radiation therapy can be used (1) as a reference for deciding on the ideal anti-microbial spectrum of the oral rinsing agent to be used in patients treated with radiation therapy for malignant tumor of the head and neck region. (2) to enhance the understanding of increase of opportunistic infection after immunochemical changes of the saliva and its relation to specific bacterial infection. (3) as a reference in prescribing prophylactic antibiotics in immunodepressed patients after radiation therapy.
Anti-Bacterial Agents
;
Bacteria
;
Bacterial Infections
;
Candida
;
Dental Caries
;
Follow-Up Studies
;
Head
;
Humans
;
Mouth Neoplasms*
;
Mucositis
;
Neck
;
Opportunistic Infections
;
Saliva
;
Seoul
10.Developmental Changes in the Activation of Signal Transduction Pathway via JNK in Rat Hippocampus after Kainic Acid-Induced Seizure.
Jong Heun KIM ; Hee Yeon JUNG ; Myoung Sun ROH ; Yong Min AHN ; Ung Gu KANG ; Yong Sik KIM ; Soo Churl CHO
Journal of Korean Neuropsychiatric Association 2001;40(5):971-980
OBJECTIVE: We observed the developmental pattern of activation of MAPK signal transduction pathways known to be activated by electroconvulsive shock(ECS) in young rat hippocampus after kainic acid(KA)-induced seizure. METHODS: We used the method of immunoblotting for examining the basal protein amount and basal level of phosphorylation of MAPK kinase(SAPK/ERK kinase -1, SEK-1), MAPK(c-Jun N terminal protein kinase, JNK), transcription factor(c-Jun) and immediate early gene proteins(Fos) in rat hippocampus at postnatal day 7, 14, and 21, respectively. We also examined the changes of phosphorylation of those proteins after kainic acid-induced seizure in the same way. RESULTS: The basal protein amounts of SEK-1, JNK, and c-Jun did not show age-dependent changes and basal level of phosphorylation of JNK and c-Jun remains unchanged throughout the early developmental period. The basal level of phosphorylation of SEK-1 was peaked at postnatal 7 days and then decreased with aging. After kainic acid-induced seizure, the change of phosphorylation of JNK was not observed but those of SEK-1 and c-Jun increased after postnatal day 14. The expression of Fos was observed at postnatal day 7 and also increased with aging. CONCLUSION: These results show that the MAPK signal transduction system in rat hippocampus matures in accordance with aging, but the process of maturation differs depending specific proteins. This study suggests the signal transduction cascade(SEK-1 - JNK - c-Jun - Fos) which is well established in cell line studies may not be applied to rat hipposcampus because we could not observe the activation of JNK after KA-induced seizure in young rat hippocampus.
Aging
;
Animals
;
Cell Line
;
Hippocampus*
;
Immunoblotting
;
Kainic Acid
;
Phosphorylation
;
Phosphotransferases
;
Protein Kinases
;
Rats*
;
Seizures*
;
Signal Transduction*