Patient-derived tumor xenograft is the transfer of primary human tumors directly into an immunodeficient mouse. Patient-derived tumor xenograft plays an important role in the development and evaluation of new chemotherapeutic agents. We succeeded in generating a patient-derived tumor xenograft of a biliary tumor obtained by endoscopic ultrasound-guided fine-needle aspiration from a patient who had an inoperable extrahepatic cholangiocarcinoma. This patient-derived tumor xenograft will be a promising tool for individualized cancer therapy and can be used in developing new chemotherapeutic agents for the treatment of biliary cancer in the future.
Aged ; Animals ; Bile Duct Neoplasms/*pathology/surgery ; Cholangiocarcinoma/*pathology/surgery ; Endoscopic Ultrasound-Guided Fine Needle Aspiration ; Heterografts/*pathology/surgery ; Humans ; Male ; Mice ; Mice, Nude ; Transplantation, Heterologous/*methods