1.Electron microscopic study on pseudorabies virus as a neurotracer.
Kyung Je CHO ; Myeong Ok KIM ; Hyung Chae KANG ; Ki Young SIN ; Jun Kyung SONG ; Heung Shik LEE ; Sa Sun CHO ; Sang Ho BAIK
Korean Journal of Anatomy 1993;26(4):469-483
No abstract available.
Herpesvirus 1, Suid*
;
Pseudorabies*
2.Expression of neutralizing proteins of pseudorabies virus using recombinant baculovirus.
Chang Hee KWEON ; Soo Hwan AN ; Jae Young SONG ; Byoung Han KIM ; Jae Jin LEE ; Young Jin KEE ; Yong Soon LEE ; Maeda SUSUMU
Journal of the Korean Society of Virology 1992;22(1):45-51
No abstract available.
Baculoviridae*
;
Herpesvirus 1, Suid*
;
Pseudorabies*
3.Seroepidemiological Survey of Aujeszky's Disease Virus in Wild Boar (Sus scrofa) and Raccoon Dogs (Nyctereutes procyonoides koreensis) in Korea.
Dong Kun YANG ; Jin Ju NAH ; Ha Hyun KIM ; Sung Suk CHOI ; Dong Jun AN ; Jong Taek KIM ; Kyoung Ae PARK ; Jae Young SONG
Journal of Bacteriology and Virology 2014;44(4):336-341
Aujeszky's disease caused by Aujeszky's disease virus (ADV) is one of the most important diseases in the pig industry. In this study, we conducted a seroepidemiological survey of ADV in wild boars and raccoon dogs in South Korea. In total, 217 wild boar sera collected between March and August 2013, and 96 raccoon dogs between 2011 and 2012 were screened for the presence of antibodies against ADV. The sero-positive rates in wild boars and raccoon dogs tested for ADV were found to be 3.55% (8/225) and 0% (0/96), respectively. The presence of virus neutralization antibody titer against ADV means that small number of wild boars was infected with ADV and AD may be circulated continuously in Korean wild boar populations, and that wild boars may act as a potential reservoir of ADV. Therefore, to achieve the declaration of AD free, effective preventive measures to block transmission of AD should be taken to the wild boars.
Antibodies
;
Herpesvirus 1, Suid*
;
Korea
;
Pseudorabies
;
Raccoon Dogs*
;
Sus scrofa*
4.Study on neural axis of rat uterus using pseudorabies virus.
Bong Hee LEE ; Wan Sung CHOI ; Kyeong Je CHO ; hae Young KWON ; Ji Hong BAE ; Jun Kyung SONG ; Heung Shik LEE ; Sang Ho BAIK
Korean Journal of Anatomy 1993;26(3):225-234
No abstract available.
Animals
;
Axis, Cervical Vertebra*
;
Herpesvirus 1, Suid*
;
Pseudorabies*
;
Rats*
;
Uterus*
5.The Neurons Related to Lateral Rectus Muscle in Cerebellum of Rats Using Pseudorabies Virus.
In Young CHUNG ; Dae Jin LEE ; Ji Myong YOO
Journal of the Korean Ophthalmological Society 2000;41(10):2107-2114
We investigated the location of neurons related to lateral rectus muscle in the cerebellum of rats. Horseradish peroxidase (HRP) and Pseudorabies virus (PRV) were injected into right lateral rectus muscle of rats. After 84~90 hours, cardiac perfusion was performed with 4% paraformaldehyde in HRP injection group and 4% paraformaldehyde-lysine-periodate in PRV injection group. Tetramethylbenzidine (TMB) neurohistochemical stain in HRP injection group and immuno-histochemical stain in PRV injection group were performed after frozen section. HRP-reactive neuronal cells were observed in ipsilateral abducens nucleus. PRV-immunoreactive neuronal cells were observed ipsilaterally in abducens nucleus, flocculus, paraflocculus, ansiform lobule, declive, dentate nucleus, infracerebellar nucleus and y nucleus and also bilaterally in nodulus, lobulus centralis, lingula, uvula vermis and fastigial nucleus. These findings show the existence of neuronal projection from the abducens nucleus to the cerebellum. This projection could be part of nerve circuits through which the cerebellum modulates visuomotor activities.
Animals
;
Cerebellar Nuclei
;
Cerebellum*
;
Frozen Sections
;
Herpesvirus 1, Suid*
;
Horseradish Peroxidase
;
Neurons*
;
Perfusion
;
Pseudorabies*
;
Rats*
;
Uvula
6.Molecular characterization and phylogenetic analysis of pseudorabies virus variants isolated from Guangdong province of southern China during 2013–2014.
Jindai FAN ; Xiduo ZENG ; Guanqun ZHANG ; Qiwen WU ; Jianqiang NIU ; Baoli SUN ; Qingmei XIE ; Jingyun MA
Journal of Veterinary Science 2016;17(3):369-375
Outbreaks of pseudorabies (PR) have occurred in southern China since late 2011, resulting in significant economic impacts on the swine industry. To identify the cause of PR outbreaks, especially among vaccinated pigs, 11 pseudorabies virus (PRV) field strains were isolated from Guangdong province during 2013–2014. Their major viral genes (gE, TK, gI, PK, gD, 11K, and 28K) were analyzed in this study. Insertions or deletions were observed in gD, gE, gI and PK genes compared with other PRV isolates from all over the world. Furthermore, sequence alignment showed that insertions in gD and gE were unique molecular characteristics of the new prevalent PRV strains in China. Phylogenetic analysis showed that our isolates were clustered in an independent branch together with other strains isolated from China in recent years, and that they showed a closer genetic relationship with earlier isolates from Asia. Our results suggest that these isolates are novel PRV variants with unique molecular signatures.
Asia
;
China*
;
Disease Outbreaks
;
Genes, Viral
;
Herpesvirus 1, Suid*
;
Pseudorabies*
;
Sequence Alignment
;
Swine
7.Seroprevalence and associated risk factors of pseudorabies in Shandong province of China.
Dongfang HU ; Lin LV ; Zhendong ZHANG ; Yihong XIAO ; Sidang LIU
Journal of Veterinary Science 2016;17(3):361-368
A cross-sectional serological study was conducted in Shandong province of China to determine the seroprevalence and risk factors associated with seropositivity due to pseudorabies virus (PRV) infection in small- and medium-sized farrow-to-finish herds following outbreaks of variant PRV strains. A total of 6,035 blood samples from 224 randomly selected herds were screened. The results showed that 25.0% of the herds and 56.7% of the serum samples were seropositive for field strains of PRV. Herds consisting of 50–100 breeding sows had higher herd seroprevalence and serum sample seroprevalence than larger herds. Both the highest herd seroprevalence and highest serum sample seroprevalence were observed in western Shandong, followed northern Shandong. Based on univariate analysis, the following risk factors were utilized in subsequent multivariable logistic regression analysis: region, herd size, weight of purchased gilts, and all-in/all-out practice. Upon multivariate analysis, region, herd size, weight of purchased gilts and all-in/all-out practice were significantly associated with PRV herd seropositivity. These findings indicate that we are facing a serious situation in the prevention and control of pseudorabies. The results could help predict the next outbreak and set out control measures.
Breeding
;
China*
;
Disease Outbreaks
;
Herpesvirus 1, Suid
;
Logistic Models
;
Multivariate Analysis
;
Pseudorabies*
;
Risk Factors*
;
Seroepidemiologic Studies*
8.Innate Immune Evasion Mechanisms of Pseudorabies Virus.
Yaozong LIU ; Ping RUI ; Rui MA ; Zengjun MA
Chinese Journal of Virology 2015;31(6):698-703
Pseudorabies is an economically important disease in a variety ot animals caused by pseudorabies virus. Since 2011, pseudorabies outbreaks occurred in many regions of China. Related researches on this virus become a hot topic in virology and veterinary. One of the difficulties for pseudorabies prevention and control is innate immune evasion. Explorations on this issue are conducive to the development of vaccine and drugs. Therefore, this review summarized the recent research progress on the mechanisms of pseudorabies virus innate immune evasion. Theoretical direction was provided on effetive prevention and control of pseudorabies owing to this review.
Animals
;
Herpesvirus 1, Suid
;
genetics
;
immunology
;
Humans
;
Immune Evasion
;
Immunity, Innate
;
Pseudorabies
;
immunology
;
virology
9.Identification of CNS neural circuitry involved in the innervation of C7 spinal nerve: a viral transsynaptic tracing study.
Hai-Feng WEI ; Liang CHEN ; Yu-Dong GU
Chinese Journal of Traumatology 2011;14(5):259-263
OBJECTIVEContralateral C7 spinal nerve transfer is a useful operation for the treatment of brachial plexus root avulsion. The recovery of the independent function at the ipsilateral side, however, depends on neural circuitry reorganization in the central nervous system (CNS). This study tried to locate the CNS neuronal elements involved in the innervation of C7 spinal nerve.
METHODPseudorabies virus (PRV, TK/gG(-), 2 microlitre), which expressed green fluorescent protein (GFP), was injected into the left C7 spinal nerve in 20 adult Sprague Dawley rats. After rats survived for 6 h, 12 h, 24 h and 36 h, the C1-C7 segments of the spinal cord and brain were processed using a polyclonal immunohistochemical antibody against PRV.
RESULTSPRV-labeled neurons were found mainly in gray matter of the C1-C7 segments of the spinal cord and at the following structures of the brain: lateral vestibular nucleus, lateral paragigantocellular nucleus, A5 cells, red nucleus, primary and secondary motor cortexes, primary and secondary somatosensory cortexes. Although located bilaterally, the PRV-labeled neurons existed predominantly in the ipsilateral side of the spinal cord and the contralateral side of the brain at 6-12 h after injection (p.i.). The number of PRV-labeled neurons in the CNS was increasing with rat's survival time and the distribution of these neurons turned bilateral with no obvious dominance to either side at 24 h and 36 h (p.i.).
CONCLUSIONBy use of transsynaptic tracing technique with PRV, the anatomically connected set of neurons, which modulates the activity of C7 spinal nerve, is located successfully in the CNS.
Animals ; Herpesvirus 1, Suid ; Neurons ; Rats, Sprague-Dawley ; Spinal Cord ; Spinal Nerves
10.Studies on neuronal tracing with pseudorabies virus.
Bi LI ; Ling ZHU ; Yuan-Cheng ZHOU ; Wan-Zhu GUO ; Zhi-Wen XU
Chinese Journal of Virology 2014;30(3):333-337
With its abilities of trans-synaptic tracing and self-replication and wide host range, pseudorabies virus (PRV) has been applied in the field of neuroanatomy since the 1970s. Four decades of PRV application have made many advances in researches on neuronal tracing with PRV. Mechanism studies focused on investigating infection of primary neurons and tracing direction in secondary neurons, while application studies focused on development of new pathological strains and innovation of tracing techniques. To date, the mechanism and application of viral tracing are not completely figured out yet. Integration of molecular biology technology will improve the efficiency in related researches.
Animals
;
Cell Tracking
;
Herpesvirus 1, Suid
;
genetics
;
physiology
;
Humans
;
Neurons
;
virology
;
Pseudorabies
;
virology