1.Expression of HSV-1 antigen in HSV-1 infected rabbit corneal cells in vitro.
Myung Kyoo KO ; Joon Kiu CHOE ; Young Tae KIM
Korean Journal of Ophthalmology 1987;1(2):81-83
This experiment was performed to determine whether cultured rabbit corneal cells were infected with HSV-1. The direct immunofluorescent method was used to detect the presence of the herpes simplex antigen in HSV-1 infected corneal epithelial cells, keratocytes, and endothelial cells in vitro with fluorescein-labeled antiserum to HSV-1. Immunofluorescent localization of the HSV-1 antigen was expressed in all three types of corneal cells.
Animals
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Antigens, Viral/*analysis
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Cells, Cultured
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Cornea/*microbiology
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Herpesviridae Infections/*microbiology
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Rabbits
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Simplexvirus/*immunology
2.Prevalence of feline herpesvirus 1, feline calicivirus and Chlamydophila felis in clinically normal cats at a Korean animal shelter.
Byeong Teck KANG ; Hee Myung PARK
Journal of Veterinary Science 2008;9(2):207-209
The prevalence of feline herpesvirus-1 (FHV-1), feline calicivirus (FCV), and Chlamydophila (C.) felis was studied in cats of an animal shelter in Korea. Total 78 cats without ocular and upper respiratory tract disease were examined. Specimens were obtained from ocular conjunctiva and oropharynx. Using multiplex polymerase chain reaction (PCR) and reverse transcription PCR, three pathogens were simultaneously detected. In examined 78 cats, 49 (63%) cats were positive for FHV-1. However, all specimens were negative for C. felis and FCV. In conclusion, many cats recovered from FHV-1 infection remain subclinical carriers in shelter environment.
Animals
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Caliciviridae/genetics
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Caliciviridae Infections/epidemiology/*veterinary
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Cat Diseases/*epidemiology/*microbiology/*virology
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Cats
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Chlamydophila/genetics
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Chlamydophila Infections/epidemiology/*veterinary
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DNA Primers/genetics
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Herpesviridae/genetics
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Herpesviridae Infections/epidemiology/*veterinary
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Housing, Animal
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Korea/epidemiology
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Prevalence
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Reverse Transcriptase Polymerase Chain Reaction