1.New limonoids isolated from the bark of Melia toosendan.
Qiong ZHANG ; Qing-Hong ZHENG ; Yi-Shu SANG ; Herman Ho-Yung SUNG ; Zhi-Da MIN
Chinese Journal of Natural Medicines (English Ed.) 2018;16(12):946-950
Two new limonoids, 12-ethoxynimbolinins G and H (compounds 1 and 2), and one known compound, toosendanin (Chuanliansu) (compound 3), were isolated from the bark of Melia toosendan. Their structures were elucidated by spectroscopic analysis and X-ray techniques. The absolute configuration of toosendanin (3) was established by single-crystal X-ray diffraction. Compounds 1-3 were evaluated for their cytotoxicity against five tumor cell lines.
Cell Line, Tumor
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Cell Proliferation
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drug effects
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Humans
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Limonins
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isolation & purification
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Melia
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chemistry
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Molecular Structure
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Plant Bark
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chemistry
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Plant Extracts
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chemistry
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isolation & purification
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pharmacology
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X-Ray Diffraction
2.Absolute configuration of podophyllotoxone and its inhibitory activity against human prostate cancer cells.
Juan LI ; Juan FENG ; Cheng LUO ; Ho-Yung Sung HERMAN ; Ren-Wang JIANG
Chinese Journal of Natural Medicines (English Ed.) 2015;13(1):59-64
Podophyllotoxone (1) was isolated from the roots of Dysosma versipellis. The structure was determined by spectroscopic analysis in combination with single-crystal X-ray analysis. The absolute configuration of compound 1 was assigned based on the Flack parameter. It showed significant inhibitory activities against human prostate cancer cells PC3 and DU145 with IC50 values being 14.7 and 20.6 μmol·L(-1), respectively. It also arrested the cells at G2/M phase. Tubulin polymerization assay showed that it inhibited the tubulin polymerization in a dose-dependent manner, and molecular docking analysis revealed a different binding mode with tubulin as compared with those known tubulin inhibitors.
Antineoplastic Agents
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chemistry
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pharmacology
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Berberidaceae
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chemistry
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Cell Line, Tumor
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Dose-Response Relationship, Drug
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G2 Phase
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drug effects
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Humans
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Hydrogen Bonding
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drug effects
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Male
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Molecular Conformation
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Molecular Docking Simulation
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Phytotherapy
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Plant Extracts
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chemistry
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pharmacology
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Plant Roots
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chemistry
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Podophyllotoxin
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chemistry
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pharmacology
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Polymerization
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Prostatic Neoplasms
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drug therapy
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Tubulin
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drug effects
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Tubulin Modulators
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pharmacology