Aim To investigate the effects of berberine on extracellular matrix in rat glomerular mesangial cells cultured under high glucose and its possible mechanism.Methods Six groups were divided according to the different experimental conditions:① Normal glucose group (NG);② Mannitol group (Mannitol);③ High glucose group (HG);④ SB203580 treatment group (HG+SB203580);⑤ Berberine low dosagegroup (HG+BBR 30 ?mol?L-1);⑥ Berberine high dosage group (HG+BBR 90 ?mol?L-1). The phospho-p38MAPK,phospho-cAMP response element binding protein (CREB) and fibronectin were detected by Western blot analysis. Results Berberine obviously decreased protein expression of fibronectin compared with high glucose group. Phospho-p38MAPK and phospho-CREB level was significantly lowered compared with that of high glucose group. Conclusion Berberine can inhibit extracellular matrix accumulation including fibronectin via p38MAPK signal pathway in treatment of diabetic nephropathy.

Objective To explore effective methods of diagnosis and treatment for delayed rupture of the spleen (DRS) through retrospective analysis of 32 cases. Methods A retrospective study was done in 32 cases with DRS. The effects of CT, ultrasound and diagnostic peritoneal lavage on diagnosis of DRS was observed and compared. Results Splenectomy was performed in 26 cases, among which one died four days after splenectomy because of complicated brain injury and six given conservative treatment under continuous monitoring discharged. The mean hospitalization duration was 18.5 days. Conclusions Diagnostic peritoneal lavage can improve the positive rate of peritoneal puncture. Ultrasound is an important method for diagnosing DRS and closely monitoring the patients receiving conservative treatment.

AIM To observe the effects of improved prescription of Didang-tang (IPDT) on exprimental atherosclerosis(AS) in rabbits and study the possible mechanism. METHODS Lipid plaque area of aortic endothelium was measured by image analysis method. lipid metabolism was measured by enzyme dynamics method, one-step method and immunization photoextinction. Plasma SOD and MDA was measured by microcontent fast detecting method and modified Ba MuGuoFu method. Ceramide was measured by thinlayer scannig; apoptosis of foam cell of aorta lipid plaque was measured by TUNEL method.RESULTS IPDT reduced lipid plaque area of aortic endothelium,regulated lipid metabolism, improved antioxidation of organism, ced ceramide content of aorta lipid plaque and apoptosis of foam cell.CONCLUSION IPDT has good effects of anti-exprimental AS,the possible mechanism maybe related to regulation of lipid metabolism and antioxidation and depression of apoptosis of foam cell.

Objective To investigate the effects of transcranial magnetic stimulation(TMS)on the recovery of neurological function and the expression of neurofilament protein(NFP)-200 in rats with cerebral infarction.Methods The string-fastening method was used to establish focal ischemia-reperfusion models in 60 Sprague-Dawley rats and the rats were divided into the TMS group and the sham TMS group(composed of 30 rats each).Then each group were divided into the treated 1 d,3 d,7 d,14 d and 21 d subgroups(composed of 6 rats each).TMS group rats were administered with 2 sessions of TMS(200 pulses total)daily,keeping on corresponding days in different groups.The recovery of neurological function and the optical density of NFP-200 by immunohistochemistry in the infarct peripheral zone in two groups were detected after therapy.Results At the 14 d and 21 d after treatment,neurological deficit scores of TMS group(2.67?0.82,1.50?0.55) were significantly lower than those of the sham TMS group(3.67?0.52,3.17?0.75)(P

Alzhelmer's disease (AD) is an important neurodegenerative disease. Recent evidence has indicated that the production and loss of the myelin,sheath are associated with AD because the particular vulnerability of oligodendrocytes produced in the late stage makes the loss of the myelin sheath take a core position in the changes of the earliest stage of AD. The loss of the myelin sheath disrupts synchronization of impulses on which normal brain functions highly depend, and ultimately results in the function disruption of cortical association regions and subsequent neuronal loss. Meanwfiile, there are diverse mechanisms that make oligodendrocytes degeneration exist in the brains of AD. Therefore, elucidating its specific mechanism may help better understanding of AD, and thus provide some help for its treatment.

Objective To evaluate the feasibility of renal artery angiography with interpolated finite impulse response (IRFR) sequence on 1.5T MR. Methods A total of 122 subjects underwent MR scan with IFIR sequence. On axial image of IFIR, bilateral signal-to-noise ratio (SNR) and breath artifact of renal artery were measured and 3D maximum intensity projection (MIP) images were reconstructed on workstation ADW 4.3, and each branch was scored according to its displaying quality. Influence of SNR, age and breath artifact on the displaying of renal artery were compared and analyzed. Results Renal segmental artery could be seen in 112 subjects (91.80%). Among them, segmental artery was showed clearly in 78 subjects (63.93%), post-segmental artery was showed in 54 subjects (44.26%) and branches of post-segmental artery was showed in 22 subjects (18.03%). There was statistical difference of renal artery scores mean rank between ≥60 years group and ＜60 years group (49.25 vs 67.48, P=0.006), as well as between the group without and with breath artifact (66.66 vs 43.35, P=0.002). No statistical difference of SNR was found among renal artery scores from 1-5 (P=0.177). Conclusion IFIR sequence is simple and safe for renal artery angiography on 1.5T MR, and different grades of renal artery branch can be showed clearly.

AIM: To observe the effect of Tangmaiantai on the plasma lipid and renal function of rat with diabetic nephropathy. METHODS: The rat model of diabetic nephropathy was established by using STZ. The effect of Tangmaiantai on rat blood glucose before meal, serum TC, TG, Ccr, BUN and urinary protein were observed. RESULTS: Tangmainantai could decrease the level of serum TC, TG, Ccr, BUN and urinary protein, comparing to that of the model group (P

In order to strengthen the management of clinical teaching, improve the teaching quality of clinical practice, the advantages of the traditional medical education, teaching reform and medical simulation were further integrated, using PDCA (Plan-Do-Check-Action) cycle model in medi-cal college students' clinical practice training and evaluation. The skills of training and evaluation were optimized and practiced by thechain model of teaching-assistance-guidance to improve the train-ing quality of interns, and make continuous improvement from the angle of teaching, learning and management and with PDCA circulation mode. Practice shows that the application of PDCA circulation management has greatly improved teachers' teaching quality and their own quality, and the students' satisfaction of practice teaching management and clinical teachers has also significantly increased. The effect is obvious.

This article described systematically the design basis,passing standard and influential factor of test score of the assessment scale of simulated clinical skills examination.Through developing and modifying OSCE examination scale design for many years,we have found that the use of 360-degree assessment for designing OSCE assessment rating scale can effectively reduce the impact on the score of examinee owing to the environment,the candidates,raters and question design,which has an important role for scientific development of OSCE assessment scales,as well as improving the reliability and validity for development of a scientific OSCE assessment.

AIM: To study the changes of the sphingomylinase activity and ceramide content in rabbit aorta of experimental atherosclerosis and investigate the effects of emodin on them. METHODS: The qualified rabbits were fed with food containing 1% cholesterol and 5% lard for 10 weeks to establish the animal models. The concentration of cholesterol (TC) was assayed by a enzyme method. Trace-fast-test method was used to test the activity of superoxide dismutase (SOD) and motified-BAMuGuoFu methods was employed to assay the content of myocardial malondialdehyde (MDA). Radiolabeled-enzyme-tracing was used to detect the activity of the sphingomyelinase,and thin-layered scanning was conducted to analyze the content of the ceramide in aorta. RESULTS: The ceramide content in aorta and the sphingomyelinase activity were markedly increased in the rabbits with experimental atherosclerosis. The increase was positively correlated with the content of TC and MDA and negatively correlated with the activity of SOD in blood. Compared to the model animals, emodin at concentration of 5 mg?kg -1 , 10 mg?kg -1 and 20 mg?kg -1 respectively reduced the area of plague on endothelium in rabbit's aortic artery and elevated the activity of SOD (P