Objective To observe the effect and safety of emergent intracoronary stenting in acute myocardial infarction (AMI) complicated by acute pump failure.Methods Fifteen patients with anterier wall AMI and apparent signs of cardiac shock were (70.1?8.4) years old on average. Their mean blood pressures were less than 85/60 mm?Hg and the ejection fractions (EF) were less than 42% on echocardiography. All the cases were performed in plantation of stenting in left anterior discending branch (LAD), among which, 4 were in right anterior artery (RCA) and 3 in left circumflex (LCX). Before and after stenting, the changes of the patients′ blood pressures, heart rates and cardiac functions tested by ultrasonography were observed. Results In 15 patients, whose LADs were completely occlusive, after stenting, the coronary arteries gained perfect reperfusion. Compared before and after stenting, the blood pressures were increased significantly after stenting [SBP (106?11.8) vs (76.2?4.9)mm?Hg, P

Objective To investigate the effect and psychological intervention of compound polyethylene glycol electrolyte powder in preoperative bowel preparation for gynecologic tumor patients. Methods According to the different drugs,nursing interventions will be January 2015 to March 2017 in third affiliated hospital of Tianjin medical university for surgical treatment of gynecological cancer patients 80 cases groups:control group with routine nursing intervention observation group with mannitol, psychological nursing and compound polyethylene glycol electrolyte with intervention;observation of two groups of patients improve psychological status and intestinal cleaning and the incidence of adverse reactions,the strict record related data and make comparative analysis. Results The psychological nursing and compound polyethylene glycol electrolyte powder(observation group)intervention in patients with gynecologic tumor significantly,the psychological state of patients with improvement than the control group,the bowel cleaning is better than the control group,the difference was statistically significant(P<0.05);two patients with adverse reaction rate comparison,the difference was not statistically significant. Conclusion The preparation of intestinal tract tumor patients before gynecologic selection of psychological nursing and intervention effect of polyethylene glycol electrolyte powder can significantly improve the psychological state,full bowel cleansing patients with high effective rate,and will not increase the adverse reactions,and is worthy of clinical application.

Objective To establish the quality control method for Bushenbanlong Tablet. Methods The content of astragaloside was determined by HPLC-ELSD. Results The linearity range was 1.44～4.32 ?g with a correlation coefficient r=0.999 9, and the average recovery was 99.69% with RSD=1.16%. Conclusion The method was simple, convient, quick and sensitive. It provide an effective method for quality control of Bushenbanlong Tablet.

Objective The High-performance thin-layer(HPTLC) chromatographic fingerprint of Saikosaponin from Chaihu(roots of Bupleurum chinense DC.) was established and fingerprints similarity of different species of Bupleurum spp.were evaluated.Methods High-performance thin-layer chromatography(HPTLC)were carried out.The chromatographic conditions were as follows:pre-coated HPTLC silica-gel plate as stationary phase,dichloromethane-methanol-ethyl acetate-water(30:40:15:3) as mobile phase(solvent system) and 2 %p-DMBA/10 %Sulfuric acid alcoholic solution as derivatization reagent.The common pattern of HPTLC fingerprints were obtained through’Chromafinger’solution software,and authentication and quality assessment were analyzed by similarity and Principle Component Analysis.Results The common pattern of the roots of Bupleurum chinense DC.consists of 19 characteristic peaks,and higher similarities existed between the roots of Bupleurum chinense DC(Bei Chaihu),B.falcatum(San-dao Chaihu) and B.scorzonerifolium Willd.(Nan Chaihu),but Nan Chaihu contains much lower total saponins than that in Bei Chaihu.The other species of Bupleurum demonstrated their different chemical distribution.The toxic species of B.longiradiatum can be easily differentiated from other spp.by comparison with the HPTLC images.Conclusion The survey showed that the main commodities of’Chaihu’in the domestic market can be attributed to’Bei Chaihu fingerprint-pattern’.The toxic species of B.longiradiatum can be easily differentiated from other spp.by comparison with the HPTLC images.

Objective To investigate the influence of pain on liver function recovery after hepatectomy Methods Clinical data of 257 patients with primary hepatocellular carcinoma were analyzed retrospectively. The patients were divided into effective analgesia group(NRS ≤ 3 points)and ineffective analgesia group(NRS > 3 points)according to the digital hierarchy(NRS). The serum liver function indexes including ALT,AST,TBiL, ALB and PT were compared in 2 groups. Results There was no significant difference in preoperative liver function between 2 groups but the levels of ALT ,AST ,PT and TBIL at 1st ,3rd ,5th and 7th days postoperatively were lower in effective analgesia group than those in ineffective analgesic group after surgery(P<0.05). The incidence of the delay of liver function was lower in effective analgesia group than that in ineffective analgesic group. Conclusion Effective analgesic treatment can reduce liver damage and accelerate liver function recovery.

ObjectiveTo investigate the effects of 5-Aza-CdR on the transcriptional regulation through methylation of the DNA promoter protocadherin 8(PCDHg) gene in pancreatic cancer cell line Capan-2.The Capan-2 retardation in growth rate and apoptosis were assessed in when administered 5-Aza-CdR and the chemotherapy agent,gemcitabine.MethodsMTT and flow cytometry were used to analyze the cell growth inhibition and apoptosis when treated with 5-Aza-CdR or in combination with gemcitabine.Methylation-specific PCR,RT-PCR and western blot were performed to detect methylation state,mRNA and protein respectively of PCDH8 gene in 5-Aza-CdR-treated Capan-2cells.Results Capan-2 cells treated with 5-Aza-CdR showed a slower growth rate,and a significant growth inhibition when given both 5-Aza-CdR in combination with gemcitabine.Compared with single drug administration and control,5-Aza-CdR together with gemcitabine can induce a stronger apoptosis signal.Different concentrations 5-Aza-CdR of were able to reverse methylation,restore mRNA and protein levels of PCDH8 in Capan-2.Conclusion5 Aza-CdR may demethylate the PCDH8 gene,which would effectively remove the gene silencing caused by high methylation,and thus induce gene mRNA transcription and protein expression to inhibit cell growth and have collaborative antitumor functions with gemcitabine.

0 05). The median duration of survival was 8 5 months, and 1 year survival rate was 32 2%. Toxicity was chiefly hematologic in the form of neutropenia. The major non hematologic toxicity was nausea or vomiting. Conclusion The GP combination chemotherapy is an active regimen for the advanced NSCLC with tolerable toxicity.

Objective To compare butorphanol or midazolam alone and combination of the two drugs in preventing etomidate-induced myoclonus during anesthesia induction.Methods One hundred sixty patients, aged 40-64 yr, with body mass index of 20-25 kg/m2, of American Society of Anesthesiologists physical status Ⅰ or Ⅱ , scheduled for elective operations under general anesthesia, were randomly allocated into 4 groups with 40 patients in each group: control group (group C), butorphanol group (group B) , midazolam group (group M) , and butorphanol combined with midazolam group (group BM).Before induction of anesthesia, butorphanol 15.0 μg/kg, midazolam 50 μg/kg, and butorphanol 7.5 μg/kg combined with midazolam 25 μg/kg were injected intravenously over 30 s in B, M and BM groups, respectively.The equal volume of normal saline was given in group C.And 2 min later, etomidate 0.3 mg/kg was injected intravenously over 1 min.The occurrence of myoclonus was recorded within 2 min after administration of etomidate, and the severity of myoclonus was assessed.Results Compared with group C, the incidence and severity of myoclonus were significantly decreased in B, M and BM groups (P＜0.05).Compared with B and M groups, the incidence and severity of myoclonus were significantly decreased in group BM (P＜0.05).There was no significant difference in the incidence and severity of myoclonus between group B and group M (P ＞ 0.05).Conclusion Butorphanol or midazolam alone produces similar efficacy in preventing etomidate-induced myoclonus during anesthesia induction, and the combination of the two drugs provides better efficacy than either alone in the patients.

Objective To explore bone formation markers in dexamethasone intervention osteocalcin (OC),bone alkaline phosphatase (BAKP),and type Ⅰ original amino terminal propcptide (PINP) relationship with bone longitudinal growth.Methods The selected thirty-three 4-week-old male SpragueDawley (SD) rats were randomly divided into two groups:the dexamethasone group (n =18) and the control group (n =15).The rats in the dexamethasone group received dexamethasone (200 μg/100 g) by intraperitoneal injection for 10 days.The rats in the control group received matching volume sodium chloride solution.All rats were weighed everyday.The rats were killed by using 10％ chloral hydration at 8 AM of 11 th day.The length of tibiae was measured.The proximal tibiae were excised,fixed and decaleified.Mter paraffin embedded,sections in 5 μm thick were cut.The growth plate sections were stained by haematoxylin-eosin (HE) histochemistry method.Total height of growth plate was measured.The rats decaptitating and the blood were collected.Serum was separated and stored in-80 ℃ refrigerator for analysis.Enzyme -linked immuno sorbent assay (ELISA) method was used to detect the rat OC,BAKP and PINP values.Results The length of growth plate and tibiae of dexamethasone group were significantly decreased contrast the control group:the length of growth plate (P =0.001),and the length of tibiae (P =0.000).There were no significant differences between two groups of the value of OC,BAKP and PINP:OC (P =0.056),BAKP (P=0.122),and PINP (P =0.169).There was positive correlation between the serum OC and the length of tibiae (r =0.454,P =0.08) in control group,the PINP and OC (r =0.521,P =0.026) in dexamethasone group.Conclusions Glucocorticoid inhibit the longitudinal bone growth,to the osteoblasts (OC,BAKP and PINP) of growing rats is not obvious.

AIM:To observe the inhibitory effect of interferon-?(IFN-?)on the growth invasiveness and metastasis of human gastric carcinoma cell line BGC-823,and mechanism of its action.METHODS:We detected the influence of IFN-? on the proliferative ability of BGC-823 in cell culture system,the cell vitality with the MTT colorimetric assay,and the cell cycle with flow cytometer(FCM).The regulatory functions of IFN-? to the expression of E-cadherin and matrix metalloproteinase-2(MMP-2)in tumor cells were estimated by immunohistochemical analysis(S-P).The ultrastructural changes of the junction among the tumor cells were observed under electron microscope.RESULTS:IFN-? can significantly inhibit the growth of human gastric carcinoma cell line BGC-823 in a dose-dependent manner.When the concentration of IFN-? was ≥106 U/L,the cell proliferation can be effectively suppressed,the suppression rate was ≥12.2%,and the blockage appeared at the phase of G_1-S of the cell cycle.Under the induction of IFN-?,the expression level of the cell E-cadherin increased while the MMP-2 decreased.The changes on ultrastructure of the cells showed the increased adhesive junctions and the relative compact structure.CONCLUSION:IFN-? can suppress the growth of human gastric carcinoma cell line BGC-823 through its influence on cell cycle.IFN-? can regulate the expression of E-cadherin and MMP-2,make the cell junction closely,so that it has the potential on restricting the invasion and metastasis of gastric carcinoma cells.