Cell Death ; Hepatocytes ; cytology ; pathology ; Humans

Hepatic Stellate Cells ; cytology ; Hepatocytes ; pathology

Apoptosis ; Hepatocytes ; pathology ; Humans ; Trans-Activators ; physiology

Fatty Liver ; pathology ; Hepatocytes ; pathology ; Humans ; Liver Diseases ; pathology

Hepatocytes ; pathology ; Humans ; Liver Diseases ; pathology ; Stem Cells ; pathology

Animals ; Hepatocytes ; Ketoconazole/pharmacology* ; Liver/pathology* ; Male ; Mice ; Testis/pathology*

Hepatocytes ; metabolism ; pathology ; Liver Diseases ; genetics ; metabolism ; pathology

No abstract available.
Cytoskeleton/*ultrastructure ; Hepatocytes/*ultrastructure ; Humans ; Liver Diseases/*pathology

OBJECTIVETo compare two models of nonalcoholic hepatocellular steatosis.

METHODSHL-7702 cells were incubated with a mixture of of unsaturated oleate acid or 50% fetal bovine serum to induce fat-overloading. Significant fat accumulation was documented by Oil Red O staining , and intracellular triglyceride levels was detected by triglyceride enzymatic assay.

RESULTSThe results showed that both 0.5 mmol/ml oleate acid and 50% FBS were able to induce nonalcoholic hepatocellular steatosis.

CONCLUSIONA nonalcoholic hepatocellular steatosis was induced by 0.5 mmol/ml oleate acid.

Cell Line ; Fatty Liver ; Hepatocytes ; pathology ; Humans ; Models, Biological

OBJECTIVETo explore the ultrastructural changes of hepatocyte fibrogenesis in cholelithiasis in biliary tract.

METHODSl0 liver biopsies were taken from the patients suffered from gallstone and choledocholithiasis during surgical treatment and the ultrastructural changes were observed under electromicroscope.

RESULTSThere were plentiful collagenous microfibrils (CMFs) grown within some hepatocytes. These CMFs distributed locally or diffusely in cytoplasm even extended into nucleus. In 7 cases numerous megamitochondrias appeared in several hepatocytes, the inclusions mimicking fibrils could be frequently seen and grew beyond the envelope. Furthermore, typical CMFs could be seen in the large microbodies, and several vesicular or cystic structures similar as fibroblast were presented in marginal areas of the hepatocytes.

CONCLUSIONSWe deduce that the fibrosed hepatocytes may be remained and take part in the hyperplasia of hepatic fibrous tissue.

Adult ; Cholelithiasis ; pathology ; ultrastructure ; Female ; Hepatocytes ; pathology ; ultrastructure ; Humans ; Liver Cirrhosis ; pathology ; Male ; Middle Aged