Hepatocytes ; cytology ; Liver, Artificial

Hepatocytes ; cytology ; Humans ; Liver ; cytology ; Stem Cells ; cytology

Hepatic Stellate Cells ; cytology ; Hepatocytes ; pathology

Cell Death ; Hepatocytes ; cytology ; pathology ; Humans

Animals ; Hepatocytes ; cytology ; Mice ; Stem Cell Transplantation ; Stem Cells ; cytology

Animals ; Endothelial Cells ; cytology ; Hepatocytes ; cytology ; Humans ; Liver Regeneration

Animals ; Cell Culture Techniques ; Hepatocytes ; cytology ; Humans ; Liver, Artificial

The temporary function replacement and intensive support play a pivotal role in the treatment of patients with acute or chronic end-stage organ failure. The hemodialysis and peritoneal dialysis have become routine techniques in the management of acute or chronic renal failure. But for the complexity of hepatic function, e.g. metabolism, biosynthesis and biotransformation for endogenous or exogenous substrates, the simulation or partial replacement of hepatic function is a great dream of bionic technologists. With the development of cell biology and biomedical material, the combination of biomaterial and the hepatocyte cultured ex vivo could provide a range of human liver-specific functions. The combination of biomaterial and viable cell was called hybrid or bioartificial liver support system (BALSS). It is a chimera of biomaterials and hepatocytes. The bionic technologists study the cell and its culture in vitro, which is the main component of BASLL. Many types of BALSS were translated into the early clinical stage. In this overview, we review the hepatocyte culture and the design of different bioreactors. It includes the immune obstacles in xeno-hemoperfusion and how to assess pre-clinical and clinical effectiveness.
Bioreactors ; Cells, Cultured ; Hepatocytes ; cytology ; Humans ; Liver, Artificial

Hepatocytes ; cytology ; Humans ; Liver Failure ; therapy ; Liver, Artificial

OBJECTIVETo investigate the biological function of hepatocyte-like cells derived from mesenchymal stem cells that isolated from human umbilical cord UC-MSCs in vitro, and to detect the changes in the immunogenicity of the differentiated hepatocyte-like cells (DHC).

METHODSTransdifferentiation of UC-MSCs into hepatic lineage in vitro was induced in modified two-step induction medium. The expressions of hepatic specific markers were detected by RT-PCR analysis and immunofluorescence staining at different time points after induction. The levels of albumin and urea in the supernatants of cultures were measured by enzyme-linked immunosorbent assay. Furthermore, the immunosuppressive property of DHC was detected by one-way mixed lymphocyte culture.

RESULTSThe mRNA and proteins of alpha fetoprotein (AFP), albumin (ALB),and cytokeratin-19 (CK-19) were expressed in naive UC-MSCs at low levels. DHC highly expressed hepatic markers AFP, ALB, CK-19, and tryptophan 2, 3-dioxygenase 14 and 28 days after hepatic differentiation and were accompanied by an increased production of ALB and urea in supernatant in a time-dependent manner. DHC did not express human leukocyte antigen DR antigen and significantly decreased the lymphocyte proliferation.

CONCLUSIONUC-MSCs are able to differentiate into functional hepatocyte-like cells in vitro, while the immunogenicity of DHC remains low.

Cell Transdifferentiation ; Cells, Cultured ; Hepatocytes ; cytology ; immunology ; Humans ; Mesenchymal Stromal Cells ; cytology ; Umbilical Cord ; cytology