No abstract available.
Hepatocytes*

Hepatocytes ; cytology ; Liver, Artificial

No abstract available.
Animals ; Hepatocytes* ; Organelles* ; Rats*

Two-stage carcinogenesis has been widely used as a method for evaluating precancerous lesion on animal experiments. Here we used diethylnitrosamine (DEN), a tumor trigger, and carbon tetrachloride (CCl4), a tumor promoter, to mimic the first two stages of hepatic carcinogenesis. Single intra-peritoneal injection of DEN 1% in combination with repeated intra-peritoneal injection of CCl4 4% for 16 weeks led to the formation of hyperplasia nodules on mouse livers. Microscopic examination also revealed dramatic changes in hepatocellular morphology and architecture: increased cell density and proliferation, abnormalities in cell division and nucleus structure. These changes were not observed in animal treated with NaCI or DEN alone. Repeated injection of CCl4 alone led to alterations on microscopic pictures but did not cause hyperplasia nodules. Our results indicate that DEN in combination of CCl4 causes abnormal proliferation of hepatic cells and evokes hepatic hyperplasia
Hepatocytes ; Cell Proliferation ; Carbon Tetrachloride

Hepatocytes ; immunology ; Liver Transplantation ; immunology

To study phenylpropanoids from Eleocharis dulcis and their hepatoprotective activities. The compounds were separated and purified from ethyl acetate part by conventional column chromatography and preparative liquid chromatography, and their structures were identified by various spectral techniques. The HL-7702 cells damage model of hepatocytes induced by APAP was used to screen and evaluate the hepatoprotective activities of these compounds. Sixteen compounds were isolated from ethyl acetate part of E. dulcis, and their structures were identified as 6'-(4″-hydroxy-3″-methoxy-phenylpropenyl)-1-(10-methoxy-phenylacetone)-1'-O-β-D-glucopy-ranoside(1), susaroyside A(2), clausenaglycoside B(3), clausenaglycoside C(4), clausenaglycoside D(5), emarginone A(6), emarginone B(7), thoreliin B(8), 4-O-(1',3'-dihydroxypropan-2'-yl)-dihydroconiferyl alcohol 9-O-β-D-glucopyranoside(9), 2-[4-(3-methoxy-1-propenyl)-2-methoxy-phenoxy]-propane-1,3-diol(10), 6'-O-(E-cinnamoyl)-coniferin(11), methyl 3-(2-O-β-D-glucopyranosyl-3,4,5,6-tetramethoxyphenyl) propanoate(12), clausenaglycoside A(13), 9-O-(E-cinnamoyl)-coniferin(14), 6'-O-(E-cinnamoyl)-syringin(15), 2'-O-(E-cinnamoyl)-syringin(16). Among them, compound 1 was a new compound. Compounds 2-16 were isolated from this plant for the first time. Among them, compounds 2 and 8 showed certain hepatoprotective activities.
Chromatography ; Eleocharis ; Hepatocytes ; Plant Extracts

The hepatoprotective effects of extracts from Phyllanthus niruri obtained by different extraction procedures were evaluated using mice with acute hepatitis, included by CCL4. The effects of lowering the serum ALT and AST of these extracts were clearly demonstrated. It was founded that the hepatoprotective effect of extract produced a protective action better than a recovery one on. Activities of Extract 3 and ChophytolR were compared
Adenoma ; Hepatocytes ; Phyllanthus ; Carbon Tetrachloride ; Pharmaceutical Preparations

Combretum species (combretaceae) are widely used as folk medicine for the treatment of hepatitis, malaria, respiratory infection and even cancer in different parts of Asia and Africa. Claimed efficacies in Thai traditional textbooks are as follows that the leaves of Combretum quadrangulare Kurz was have been used as a treatment of wounds. In this study, we researched the action of Tram bau on hepatotoxicity. The centrilobular localization of CCl4 induced hepatic necrosis. The results showed that Trambau decoction with dosage 0,2 ml recovered the hepatic injury.
Recovery of Function ; Poisoning ; Infection ; Combretaceae ; Hepatocytes

144 HCC patients diagnosed histopathologically/cytologically with fine needle aspiration under sonographic leading were studied ultrasonically. 52.08% of patients had 1 lesion, 44.45% had more 2 lesions and only 3.47% had dispersive lesions. Of 75 cases with 1 lesion, 21 had under 5cm lesion, 42 from 5 to 10cm and 12 above 10cm. The relation between dimention of the lesion and its ultrasonic echo-pattern was investigated
Carcinoma, Hepatocellular ; Hepatocytes ; Biopsy, Fine-Needle

Apoptosis ; Hepatocytes ; pathology ; Humans ; Trans-Activators ; physiology