3.Occult hepatitis B virus infection.
Chang-yun CHEN ; Jian-rui WU ; Xin-yu ZHU ; Shuo-yi WANG
Chinese Journal of Hepatology 2005;13(11):873-875
4.Microarrays for detection of HBV and HDV.
Zhao-Hui SUN ; Wen-Ling ZHENG ; Bao ZHANG ; Rong SHI ; Wen-Li MA
Chinese Journal of Hepatology 2004;12(9):563-564
7.Analysis of suspected hepatitis B virus genotype I from one patient in Guizhou province.
Xing-lin YANG ; Hai HUANG ; Yi-guang WANG ; Yong-fu LI ; Jie TIAN
Chinese Journal of Hepatology 2012;20(7):545-546
Adult
;
China
;
epidemiology
;
Genes, Viral
;
Genotype
;
Hepatitis B
;
epidemiology
;
virology
;
Hepatitis B virus
;
classification
;
genetics
;
isolation & purification
;
Humans
;
Male
8.Multiplex PCR normalization and parallel detection of HBV and HCV.
Chinese Journal of Experimental and Clinical Virology 2003;17(1):50-54
<b>BACKGROUNDb>To design and establish a method of multiplex PCR normalization and parallel detection of HBV and HCV.
<b>METHODSb>Using two pairs of primers, one inner and the other outer, each having a 20 bp common sequence, the authors amplified target DNA for two rounds. All products would have this common sequence. Using this common sequence as primer the authors performed further amplification. Finally, multiplex PCR was normalized to a single PCR style and eliminated multiple factors disturbance. Four kinds of nucleic acid extraction method were compared. Multiplex PCR normalization was established and optimized by using orthogonal design by analysing 6 kinds of key factors. The method was evaluated by detecting 28 samples of HBV and HCV.
<b>RESULTSb>The sensitivity, specificity, diagnostic idex and efficiency for HBsAg, HCV antibody positive patients were 83.3%, 70.0%, 153.3%, 72.2% respectively. The sensitivity, specificity, idex and efficiency for HBsAg positive patients were 78.6%, 80.0%, 158.6%, and 79.2%, respectively. The sensitivity, specificity, idex and efficiency for HCV antibody positive patients were 75.0%, 90.0%, 165.0% and 83.3% respectively.
<b>CONCLUSIONSb>The multiplex PCR normalization method may have potential applicability in parallel amplification of multiple genes of pathogens.
DNA, Viral ; analysis ; Hepacivirus ; isolation & purification ; Hepatitis B ; virology ; Hepatitis B virus ; isolation & purification ; Hepatitis C ; virology ; Humans ; Polymerase Chain Reaction ; methods ; RNA, Viral ; analysis ; Sensitivity and Specificity ; Superinfection
9.Surveillance of viral contamination of invasive medical instruments in dentistry.
Lin-fu ZHOU ; Hai-hong ZHU ; Jun LIN ; Min-jun HU ; Feng CHEN ; Zhi CHEN
Journal of Zhejiang University. Science. B 2006;7(9):745-748
<b>OBJECTIVEb>To investigate the viral contamination of invasive medical instruments in dentistry and to provide health administrative institutions with surveillance data.
<b>METHODSb>Sterilized samples were randomly collected from the department of dentistry to detect HBV-DNA, HCV-RNA, HIV-RNA and HBsAg.
<b>RESULTSb>Of the invasive medical instruments that were sterilized with 2% glutaraldehyde, one of the samples was positive for HBV-DNA, and another sample was positive for HBsAg.
<b>CONCLUSIONb>Though massive virus contamination of invasive medical instruments in dentistry has been reduced to a low level, the occurrence of contamination still remains.
DNA, Viral ; analysis ; Dental Instruments ; virology ; Equipment Contamination ; HIV ; isolation & purification ; Hepacivirus ; isolation & purification ; Hepatitis B Surface Antigens ; analysis ; Hepatitis B virus ; isolation & purification ; Humans ; RNA, Viral ; analysis
10.Role of hepatitis B virus infection in pathogenesis of IgA nephropathy.
Nian-song WANG ; Zhao-long WU ; Yue-e ZHANG ; Mu-yi GUO ; Lü-tan LIAO
Chinese Journal of Preventive Medicine 2003;37(1):37-40
<b>OBJECTIVEb>To clarify the relationship between hepatitis B virus (HBV) infection and IgA nephropathy (IgAN).
<b>METHODSb>HBV antigen (HBAg) in renal tissues of the patients with IgAN was detected by immunohistochemical technique, the carrier status and localization of HBV DNA in renal tissues were determined by Southern blot analysis and in situ hybridization.
<b>RESULTSb>Serum HBsAg was detected in 18 of the 100 patients with IgAN (18%), HBAg was detected in 31 of 100 patients (31%) in their renal tissue and in 20 of 31 patients (65%) in their glomeruli, and both HBsAg and HBcAg were detected in 10 of 31 patients (32%), respectively. HBcAg was also found in tubular epithelia (45%, 14/31) and renal interstitium (6%, 2/31), respectively. Five of six cases were proved to be positive of integrated-form HBV DNA in their renal tissue by Southern blot analysis. In situ hybridization demonstrated that HBV DNA was 8/8 and 6/8 positive in their renal tubules and glomeruli of all eight specimens, localized in the nucleus of tubular epithelial cells, glomerular mesangial cells, as well as infiltrated interstitial lymphocytes.
<b>CONCLUSIONb>HBV infection closely related with IgAN and HBV infection might be involved in pathogenesis of IgAN.
Adolescent ; Adult ; Female ; Glomerulonephritis, IGA ; complications ; virology ; Hepatitis B ; complications ; Hepatitis B Antigens ; analysis ; Hepatitis B virus ; isolation & purification ; Humans ; Male ; Middle Aged
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