1.Occult hepatitis B virus infection: clearance or disguise?.
Clinical and Molecular Hepatology 2014;20(3):249-250
No abstract available.
DNA, Circular/*analysis
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Female
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Hepatitis B/*pathology
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Hepatitis B Surface Antigens/*genetics
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Hepatitis B virus/*metabolism
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Humans
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Male
2.Expression of Caspase-3 increased in children with hepatitis B virus-associated membranous nephropathy.
Chinese Journal of Pediatrics 2007;45(7):539-541
Caspase 3
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metabolism
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Child
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Female
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Glomerulonephritis, Membranous
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enzymology
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etiology
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pathology
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virology
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Hepatitis B
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complications
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pathology
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Hepatitis B virus
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Humans
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Male
3.The influence of HBsAg expression in liver tissue on the postoperative recurrence of HCC patients.
Qiao Mei LI ; Hua Bang ZHOU ; He Ping HU ; Yuan HUANG ; Huan ZHAO ; Shang Wen YANG
Chinese Journal of Oncology 2022;44(4):347-353
Objective:b> To investigate the influence of HBsAg expression in peritumoral tissue of hepatocellular carcinoma (HCC) patients on their postoperative recurrence. Methods:b> The HCC patients treated in Shanghai Eastern Hepatobiliary Surgery Hospital from October 2009 to August 2010 were selected. The clinicopathological data and adjacent tissues of 718 patients were collected, and dextran polymer immunohistochemical staining was used to detect the expression of HBsAg in adjacent tissues. According to the expression of HBsAg in adjacent tissues, the tissues were divided into HBsAg positive group and HBsAg negative group. Kaplan-Meier method and Log rank test were used for survival analysis, and Cox regression model was used for multivariate analysis. Results:b> Among the 718 patients in the whole group, 153 were HBsAg negative and 565 were HBsAg positive. There was a statistically significant difference in serum HBV DNA level between HBsAg-positive and HBsAg-negative patients (P<0.001). The number of patients with serum DNA≥2 000 IU/ml and<2 000 IU/ml in HBsAg negative group were 52 and 93, while the patients in HBsAg positive group were 325 and 205. The cumulative recurrence rates of all patients at 1, 3, and 5 years after surgery were 30.2%, 54.3%, and 62.7%, respectively. The expression of HBsAg was related to the recurrence (P=0.038). Multivariate analysis showed that γ-GT, PT, multiple tumors, tumor length, and portal vein invasion were independent risk factors for recurrence of HCC (P<0.05). In HBeAg-negative patients with low viral load (HBV DNA <2 000 IU/ml) and without cirrhosis, the recurrence rates of HBsAg-positive patients were 14.3% and 31.0% at 3 and 5 years, respectively, compared with HBsAg negative patients (all 0), the difference was statistically significant (P=0.021). Conclusion:b> The positive expression of HBsAg in peritumoral tissue increases the postoperative recurrence risk of HCC patients.
Carcinoma, Hepatocellular/pathology*
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China
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DNA, Viral/analysis*
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Hepatitis B Surface Antigens
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Hepatitis B virus/metabolism*
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Humans
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Liver Neoplasms/pathology*
4.The expression of macrophage migration-inhibitory factor in HBV-infection-related liver diseases.
Xiao-hui YU ; Cong LIU ; Xiong-zhi WU ; Fang-xin ZHANG ; Lian-san ZHAO
Chinese Journal of Hepatology 2007;15(1):60-61
Adolescent
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Adult
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Aged
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Female
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Hepatitis B
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metabolism
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pathology
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Hepatitis B virus
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Humans
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Liver Diseases
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metabolism
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pathology
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virology
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Macrophage Migration-Inhibitory Factors
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metabolism
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Male
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Middle Aged
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Young Adult
5.Increased expression of inducible nitric oxide synthase in chronic hepatitis B patients is correlated with histopathological grading and staging.
Juan WU ; Kai WANG ; Li-yan HAN ; Yu-chen FAN
Chinese Journal of Experimental and Clinical Virology 2008;22(1):57-59
<b>OBJECTIVEb>To investigate the intrahepatic expression of inducible nitric oxide synthase (iNOS) in patients with chronic hepatitis B (CHB) and its relation to liver histopathology.
<b>METHODSb>The intensity and distribution of the immunohistochemical staining of intrahepatic iNOS were studied in the liver biopsy specimens obtained from 74 patients with CHB and statistical analyses were performed between intrahepatic iNOS and ALT, HbeAg, HBV DNA grading of liver inflammation and staging of fibrosis. Seven histologically normal liver sections were used as a control group.
<b>RESULTSb>Compared with the control group, the intrahepatic iNOS immunoexpression was significantly higher in patients with CHB (P < 0.05), iNOS immunoreactivity was observed mainly in hepatocytes showing a predominant cytoplasmic staining, with the positive liver cells distributed diffusely throughout the hepatic lobule. Immunopositive staining could also be detected in Kupffer cells, sinusoidal lining cells and vascular endothelial cells. Compared with patients with normal ALT, the hepatocellular iNOS immunoexpression was significantly higher in patients with elevated ALT (P < 0.05) and the iNOS immunoexpression was significantly correlated with the serum level of ALT (r=0.601, P=0.000). Statistical analysis also showed that the intrahepatic iNOS immunoexpression was positively correlated with the grading of liver inflammation and the staging of liver fibrosis (r=0.660, P=0.000; r=0.507, P=0.000). No significant correlation between iNOS and HBeAg and HBV DNA was detected. CONCLUSION The intrahepatic expression of iNOS is elevated in chronic hepatitis B patients and correlated well with the severity of the disease, which indicated that inducible nitric oxide synthase may have a critical role in the pathogenesis of chronic viral hepatitis B.
Adult ; Alanine Transaminase ; metabolism ; DNA, Viral ; metabolism ; Female ; Gene Expression Regulation, Enzymologic ; Hepatitis B Antigens ; metabolism ; Hepatitis B virus ; metabolism ; Hepatitis B, Chronic ; enzymology ; metabolism ; pathology ; virology ; Hepatocytes ; metabolism ; Humans ; Male ; Nitric Oxide Synthase Type II ; metabolism
6.The Degrees of Hepatocyte Cytoplasmic Expression of Hepatitis B Core Antigen correlate with Histologic Activity of Liver Disease in the Young Patients with Chronic Hepatitis B Infection.
Tae Hyeon KIM ; Eun Young CHO ; Hyo Jeong OH ; Chang Soo CHOI ; Ji Woong KIM ; Heung Bae MOON ; Haak Cheul KIM
Journal of Korean Medical Science 2006;21(2):279-283
Subcellular localizaton of HBcAg have been found to be related to the activity of liver disease and HBV replication. The aim of this study was to determine whether the degree of expression of HBcAg in the hepatocyte nucleus and cytoplasm reflects the level of viral replication and histological activity in chronic HBV infection. A total of 102 patients with biopsy proven chronic hepatitis B were included. There was a highly significant correlation between the levels of HBV DNA in serum and the degree of expression of HBcAg in the nucleus for HBeAg-positive(p=0.000) and negative patients(p=0.04). There was a highly significant, correlation between the degrees of expression of HBcAg in hepatocyte cytoplasm and histologic activities (p<0.01) for HBeAg-positive patients. The degrees of expression of HBcAg in the hepatocyte cytoplasm correlated positively with the lobular activities (p<0.01), but not correlated with the portal activity and fibrosis for HBeAg-negative patients. In conclusion, in the young patients with chronic B viral hepatitis, the degree of expression of HBcAg in the hepatocyte nucleus may affect viral load, and the degree of expression of HBcAg in the hepatocyte cytoplasm may affect histologic activities of liver disease.
Male
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Liver/pathology/virology
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Humans
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Hepatocytes/pathology/*virology
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Hepatitis B, Chronic/*pathology/*virology
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Hepatitis B e Antigens/metabolism
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Hepatitis B Core Antigens/*metabolism
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DNA, Viral/blood
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Cytoplasm/virology
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Cell Nucleus/virology
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Adult
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Adolescent
7.Gene expression profile of peripheral blood monocyte in patients with fulminant hepatitis B by cDNA microarray.
Ying-xia LIU ; Guo-ling HU ; De-ming TAN ; Shu-ya HE ; Shuang-hu LIU
Chinese Journal of Hepatology 2003;11(3):153-155
<b>OBJECTIVEb>To investigate peripheral blood monocyte (PBMC) gene expression profile in patients with fulminant hepatic failure (FHF) by cDNA microarray.
<b>METHODSb>Microarrays consisting of 8,192 human cDNAs and labelled cDNAs prepared from PBMC in both 10 FHF patients and 10 asymtomatic surface antigen carriers (ASC) were applied to analyze gene expression. Relative ratios of gene expression in individuals were obtained by comparing the hybridization results, by GenePix 4000B scanning and by ImaGene3.0 software analysis, of Cy5-labelled cDNA from FHF patients with those of Cy3-labelled cDNA from ASC.
<b>RESULTSb>249 genes out of 8,192 were identified differently, at least two times. Most of the genes (79%) involved in cell signaling transduction, cell cycles, metabolism, inflammatory response and apoptosis, whose mRNAs were differently regulated.
<b>CONCLUSIONSb>These results suggest that HBV infection alters a broad range of cellular genes expression during developing into FHF and provide a framework for future functional study on the genes expressed differently.
DNA, Complementary ; genetics ; Female ; Gene Expression ; Gene Expression Profiling ; Hepatitis B ; genetics ; pathology ; Hepatitis B, Chronic ; genetics ; pathology ; Humans ; Leukocytes, Mononuclear ; metabolism ; Male ; Oligonucleotide Array Sequence Analysis
8.Study of serum level of cortisol and peripheral T lymphocyte subsets state in the hepatitis B virus carriers.
Xiao-peng FAN ; Kai WANG ; Yu-chen FAN
Chinese Journal of Experimental and Clinical Virology 2008;22(5):330-332
<b>OBJECTIVEb>To study of serum level of cortisol and peripheral T lymphocyte subsets state in the hepatitis B virus (HBV) carriers.
<b>METHODSb>Sixty chronic HBV carriers and ten healthy controls were all enrolled in this present study. Serum expression of cortisol was determined by radioimmunoassay, and also flow cytometry was performed to evaluate peripheral blood T lymphocyte subset.
<b>RESULTSb>Compared with those in normal controls, the serous levels of cortisol in chronic HBV carriers were significantly elevated, while there was no distinct difference in the proportion of CD4+ T lymphocytes ( P > 0.05) with the decreased odds of CD4+/CD8+ lymphocytes( P < 0.05) and obvious higher proportion of CD8+ T lymphocytes( P < 0.05). In comparison between HBeAg positive group and HBeAg negative group, the serous levels of cortisol of the former group were significantly higher ( P < 0.05), and so proportion of CD8+ T was too ( P < 0.05). However, there is no significant differences in the proportion of CD4+ T lymphocyte ( P > 0.05).
<b>CONCLUSIONb>The elevated serum cortisol and increased CD8+ T lymphocytes subsets in the chronic HBV carriers, suggested that there was disturbance of endocrine-immune response in the chronicity of HBV infection.
Adult ; Carrier State ; immunology ; pathology ; virology ; Female ; Hepatitis B ; blood ; immunology ; metabolism ; pathology ; Hepatitis B virus ; immunology ; Humans ; Hydrocortisone ; blood ; immunology ; Male ; T-Lymphocyte Subsets ; immunology
10.Association of Polymorphism in MicroRNA 604 with Susceptibility to Persistent Hepatitis B Virus Infection and Development of Hepatocellular Carcinoma.
Jae Youn CHEONG ; Hyoung Doo SHIN ; Sung Won CHO ; Yoon Jun KIM
Journal of Korean Medical Science 2014;29(11):1523-1527
MicroRNA polymorphisms may be associated with carcinogenesis or immunopathogenesis of infection. We evaluated whether the mircoRNA-604 (miR-604) polymorphism can affect the persistence of hepatitis B virus (HBV) infection, and the development to hepatocellular carcinoma (HCC) in patients with chronic HBV infection. A total of 1,439 subjects, who have either past or present HBV infection, were enrolled and divided into four groups (spontaneous recovery, chronic HBV carrier without cirrhosis, liver cirrhosis and HCC). We genotyped the precursor miR-604 genome region polymorphism. The CC genotype of miR-604 rs2368392 was most frequently observed and T allele frequency was 0.326 in all study subjects. The HBV persistence after infection was higher in those subjects with miR-604 T allele (P=0.05 in a co-dominant and dominant model), which implied that the patients with miR-604 T allele may have a higher risk for HBV chronicity. In contrast, there was a higher rate of the miR-604 T allele in the chronic carrier without HCC patients, compared to those of the HCC patients (P=0.03 in a co-dominant model, P=0.02 in a recessive model). The T allele at miR-604 rs2368392 may be a risk allele for the chronicity of HBV infection, but may be a protective allele for the progression to HCC in chronic HBV carriers.
Adult
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Aged
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Aged, 80 and over
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Base Sequence
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Carcinoma, Hepatocellular/etiology/*genetics/pathology
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Case-Control Studies
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Demography
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Female
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Gene Frequency
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*Genetic Predisposition to Disease
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Genotype
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Hepatitis B Antibodies/blood
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Hepatitis B Surface Antigens/blood
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Hepatitis B e Antigens/blood
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Hepatitis B virus/metabolism
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Hepatitis B, Chronic/complications/*genetics/virology
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Humans
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Liver Neoplasms/etiology/*genetics/pathology
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Male
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MicroRNAs/*genetics/metabolism
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Middle Aged
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Polymorphism, Single Nucleotide
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Risk Factors