No abstract available.
Antigens, Surface* ; Hepatitis B* ; Hepatitis*

Composite recombinant antigens were constructed from diagnostically relevant antigenic regions derived from the hepatitis C virus (HCV) core, NS3, NS4 and NS5 proteins. Antigenic properties of each antigenic region included into these composite antigens were preliminary evaluated by testing recombinant proteins containing the HCV protein fragments of different sizes encoded by overlapping PCR fragments. Immunoreactive recombinant proteins were tested by EIA against a large panel of anti-HCV positive and negative serum specimens to identify the most immunoreactive and specific proteins. Three composite antigens were designed based on this analysis. The fist composite protein was composed of one antigenic region from core, NS3 and NS4. The second protein contained an additional NS4 region, while the third protein contained all immunodominant antigenic regions identified in this study. After EIA analysis for the detection of anti-HCV, each composite protein showed almost identical results compared to combining each of the corresponding individual proteins.
hepatitis C ; antigens ; viruses

No abstract available.
Hepatitis B e Antigens* ; Hepatitis B virus* ; Hepatitis B* ; Hepatitis*

No abstract available.
Hepatitis B e Antigens* ; Hepatitis B Surface Antigens* ; Hepatitis B virus* ; Hepatitis B* ; Hepatitis*

No abstract available.
Antibodies* ; Hepatitis B Surface Antigens* ; Hepatitis* ; Prevalence*

Background: Vietnam is located in the endemic region of hepatitis B virus (HBV) infection, but no data of occult HBV infection was reported at present. Objectives: To investigate the prevalence of occult HBV infection in different ethnics of people and generations. Subjects and method: 80 voluteers with HbsAg negative from five different ethnics: Kinh, Tay, Mong, Giay and Dao in a Chino \ufffd?Vietnamses border province (Lao Cai) were enrolled in the study. After HBV-DNA was extracted, nested PCR of S gene and of Core-promoter/Pre-core region were used to detect HBV-DNA. Specifying nucleotide sequence was confirmed by direct sequencing. Results:The prevalence of occult HBV infection in population study was very high 73/80 (91,3%) by nested PCR of Core-promoter/Pre-core, significantly more sensitive than nested PCR of S gene (26,3%) (p<0,0001). The prevalence of occult HBV infection was notdifferent between ethnics of people or between children, adults. Conclusion: Occult HBV infection in Vietnamese is very common; however, nationwide further studies should be carried out to confirm this preliminary results and evaluate the impact of occult HBV infection in Vietnam.
Hepatitis B virus ; Hepatitis B Surface Antigens ;

Hepatitis B virus (HBV) transmission mainly occurs for the public active persons. Test diagnose HBV, that is cheap cost, adjacent easy and effective relatively result, is HBsAg and anti-HBs. If test is positive HBs, body is infecting HBV. If test is positive anti-HBs, body were infected HBV. Study on 1138 people with age of from 0-60 year old, were divided into 10 groups according to WHO. Results shown that positive HBsAg related with age, group of age from 0-29 has the directly proportional rate, age increased according to disease increased. But age of from 30 - 60, the disease related with inversely proportional rate with age. From 20-60 years old, HBV infected rate is fluctuated from 40-45%.
Hepatitis B Surface Antigens ; Hepatitis B virus

The indicators are usually used in diagnosis of Hepatitis B were HBsAg, HBeAg antigens and SGOT, SGPT. 150 persons with the positive HBsAg among persons who was waiting for injection of Hepatitis B vaccine in the Highland institute of epidemiology tested have shown that there was closely relation between these indicators which demonstrated that there was an interaction between person and pathogen via immunological and biochemical reactions.
Hepatitis B virus ; Hepatitis B Surface Antigens

51 patients with acute hepatitis B were studied at Infectious and Tropical Diseases Department, Hue Central Hospital with criteria of clinical features, transaminase 5 times higher than normal upper limit and positive HbsAg at hospitalized time. 62% of them admitted hospital from 1 to 14 days after developed disease, reminder admitted hospital later than 15thday. Symptoms: 45,1% patients no fever; 88,2% oligouria and dark urine; 58,8% without swell of liver; 9,8% bleeding; 9,8% faint. Test results: there were 11% of patients with prothrombin <60%, 82,4% with bilirubin <340µg/l. 62,7% with De Ritis<1, 35,3% with HbeAg(+) and 33,3% with HbsAg (+) remained for 6 months. HbsAg positive remained for 6 months is associated with the group of patients admitted hospital later than 15thday (X2=5.1, P<0,05), the group with no fever (X2=10.1, P<0,05), the group with oligouria and dark urine (X2=13.6, P<0,05) and the group with jaundice (X2=5.1, P<0,05). The associations also existed between bilirubin level <340µg/l and the remained HbsAg (+) for 6 months and HbsAg (+) at the patients’ admission and the remained HbsAg (+) for 6 months (X2=5.5, P<0,05 and X2=31.3, P<0,05, respectively). The latter is strongly positive association.
Hepatitis B ; Hepatitis B Surface Antigens

Research on 39 patients with acute virus hepatitis and 33 patients with chronic virus hepatitis, 9 patients have clinical cirrhosis symptoms. All of them suffer HbsAg (+) and liver function disorderly. Results: in lower 40 ages group, the incidence of acute virus hepatitis higher than chronic virus hepatitis and cirrhosis. Man with hepatitis and cirrhosis higher than woman byfar (80,2% vs 19,8%). Reducing aglobulia, pigment blood ……….. in man patients with acute virus hepatitis, chronic virus hepatitis are higher than healthy people. Number of Glomerule in chronic hepatitis and cirrhosis group and man with acute hepatitis reduce significantly compare health people. In suffer acute virus hepatitis, there are different significantly between nomal acute virus hepatitis and severe acute virus hepatitis
Hepatitis ; Liver ; Hepatitis B Surface Antigens