1581 person from 2 different geographic areas of Thanh Hoa province participated in the serosurvey of hepatitis B and C (HBV and HCV) virus in August 1998. They were divided into 4 age groups representative of different modes of viral transmission; 2 infant groups: 9-18 months old and 4-6 years old, adolescent group 14-16 years old and adult group 25-40 years old. Nearly half of the surveyed people were exposed to HBV, the rate of HBsAb positive increased with age and up to 81,4% of adults. The rate of HBsAg carriers was 17% there was no significant difference between age groups. More than 85% of infant HBsAg carriers were HBeAg positive, these rates on adolescent and adult were lower. HBsAb increased with age and only 3,4% of infant 9-18 months old had this antibody. Only 6 samples and no one of infant were positive among 1563 anti-HCV tests.
Hepatitis B ; Hepatitis C ; Hepatitis, Viral, Human

<b>OBJECTIVEb>To observe the status of occult hepatitis B virus infection in chronic viral hepatitis patients with non-A to E hepatitis virus infection and explore the diagnostic value of fluorescence quantitative polymerase chain reaction (FQ-PCR) technique for occult hepatitis B virus infection.

<b>METHODSb>The amount of HBV-DNA in serum and liver tissue from 57 patients with non-A to E hepatitis virus infection who were diagnosed as chronic viral hepatitis by Menghini method liver biopsy were detected by using FQ-PCR technique, then the relation between the viral load of HBV DNA in liver tissue and hepatic inflammatory activity were analyzed.

<b>RESULTSb>Thirteen (22.81%), 22 (38.60%) patients were positive for HBV DNA in serum and liver tissue, respectively. The positive rate and the level of HBV DNA quantity in liver tissue were significantly higher than those in serum; HBV DNA was found positive in both serum and liver tissue in 13 cases, negative in both serum and liver tissue in 35, positive in liver tissue but negative in serum in 9, and in none of the cases HBV DNA was positive in serum but negative in liver tissue (P < 0.01). The logarithmic value of HBV DNA from 13 patients in liver tissue and in serum was respectively: (6.62 +/- 1.21) copies/g vs.(4.03 +/- 1.06) copies/ml, P < 0.01. The hepatic lesions of all HBV DNA positive patients were active pathologic changes, but the level of HBV DNA in liver tissue was not significantly correlated with the grade of hepatic inflammation activity (P > 0.05).

<b>CONCLUSIONb>Occult HBV infection is the etiology of part of the chronic viral hepatitis patients with non-A-E hepatitis virus infection. Missed diagnosis will occur if diagnosis of hepatitis B is only based on detection of serum HBV markers. It is useful for improvement of the diagnostic level of HBV infection via detection of HBV DNA quantitatively in serum especially in liver tissue of chronic viral hepatitis patients with non-A-E hepatitis virus infection by using FQ-PCR technique. The chronic viral hepatitis patients with occult HBV infection should be also given effective anti-viral therapy.

Carrier State ; physiopathology ; DNA, Viral ; Hepatitis B ; physiopathology ; Hepatitis B Surface Antigens ; immunology ; Hepatitis B virus ; physiology ; Hepatitis C ; physiopathology ; Hepatitis D ; physiopathology ; Hepatitis E ; physiopathology ; Hepatitis, Viral, Human ; physiopathology ; Humans

Biomarkers ; blood ; Elasticity Imaging Techniques ; methods ; Fatty Liver ; complications ; Hepatitis B, Chronic ; complications ; Hepatitis C, Chronic ; complications ; Hepatitis, Viral, Human ; complications ; Humans ; Liver Cirrhosis ; diagnosis ; diagnostic imaging ; etiology ; Predictive Value of Tests ; Sensitivity and Specificity ; Severity of Illness Index

A survey on 170 health cadres and 220 normal people has shown that the rates of the positive HBsAg, possitive HBs and hepatitis B viral being infection in the health cadres were 12.35; 52.36; 64.71, respectively. These rates in the normal people were 7.73; 31.13; 39.09 respectively. As results the rate of Hepatitis B viral infection in the health cadres was higher than this in normal people.
Hepatitis B ; Hepatitis, Viral, Human

30 patients with the active chronic hepatitis B and 30 patients with the ascitic cirrhosis due to hepatitis B from the Army Central Hospital 108 and the National Institute of Clinical Medicine and tropical diseases participated to a study. The results have shown that the coagulation factors II, V, VII, and X in patients with the active chronic hepatitis B were moderately reduced during the exacerbation of the disease and gradually recovered to a normal level or almost normal level during the stable disease. While these factors in the patients with ascitic cirrhosis were severely, unreversible reduced through the progress of disease. During the exacerbation of the diseases. The extend of reduction of the above coagulation factors in patients of the ascitic cirrhosis was more severe than these in patients with the active chronic hepatitis B.
Hepatitis B ; Hepatitis, Viral, Human

A cross-sectional study was performed from April to December 1999 to evaluate the changes in antibody levels after HBV infection. 242 medical students were screened. Among these, 49 students (mean age 20.7+/-1.25 years, weight 52.3+/-8.1kg) were infected with wild HBV, as confirmed by presence of anti-HBs and anti-HBc and absence of HBsAg. In the first visit, the mean serum concentration of anti-HBs was 323mUI/mL. After 9 months, there was insignificant change in this antibody level with the mean was 303mUI/mL (p=1).
Hepatitis B ; Hepatitis, Viral, Human

An analysis of 58/218 patients with the possitive HBsAg(+) consulted in the Ngoc Khanh medical and health advice center was implemented. The objective of the study was to find the clinical and paraclinical features, discuss some aspects of the early detection according to the a typical symptoms, the high rate of both HBsAg (+) and antiHBs(+) as well as the transmission resources and treatment in the future.
Hepatitis B ; Hepatitis, Viral, Human

The most common infected disease in the world in known as hepatitis virus, especially hepatitis B virus (HBV). Vietnam is a country that has a high ratio of HBV infected people. In this experiment, we tested 927 workers from 18 to 25 years old. HBsAg, detected by ELISA techniques, is used by many researcher as a sigh to detect nonsymptom HBsAg carries. The common HBsAg ratio is 3.56%. The highest infected aged group ranged from 20 to 30 years old (3.96%). The lowest aged group up to 20 years old (2.00%). The HBsAg(+) ratio of male (4.31%) is higher than of female (1.74%) with p<0.05. Hanoi is the city that has the lowest HBsAg (+) ratio (2.73%), and Ha Tay is highest (5.17%).
Hepatitis B ; Hepatitis, Viral, Human

An investigation were carried out on 42 patients of acute hepatitis B (male: 34; female: 8; age: 17-50) at Institute of Clinical Medicine and Tropical Diseases from May to Dec, 1997. Hematology biochemistry tests are determined. The results: the increased concentration of AFP in serum of acute hepatitis B patients (87,95 +/_ 12,89 ng/ml) in compared with normal person. The high level of AFP of 10ng/ml on 20/42 patients (47, 6%). There is a correlation of AFP level, transaminase with AST in the phases I and III and ALT in the phases III. AFP correlates with bilirubin TP during acute hepatitis.
Hepatitis B ; Hepatitis, Viral, Human

Hepatitis B is a common disease in Vietnam; 15-20% of population carries HbsAg. The investigation of etiology and prognosis should consider the circulate immunal complex (CIC). A study on 65 patients with ages of 17-60 in the Institute of Clinical Medicine and Tropical Diseases during 1997-1998 has shown that the CIC in patients with acute hepatitis B was significantly increased comparing with this normal people. 63% of patients with acute hepatitis B experienced the increase of CIC 250. There was a relation between CIC and some hepatic functional tests in the same time.
Hepatitis B ; Hepatitis, Viral, Human