Hepatic Stellate Cells ; immunology

Hepatic Stellate Cells ; metabolism ; Humans ; Signal Transduction

Hepatic Stellate Cells ; Humans ; Liver Cirrhosis

Hepatic Stellate Cells ; cytology ; Hepatocytes ; pathology

Hepatic Stellate Cells ; metabolism ; Humans ; Signal Transduction

Hepatic Stellate Cells ; Liver Cirrhosis ; therapy

Cell Separation ; methods ; Cells, Cultured ; Hepatic Stellate Cells ; cytology ; Humans

Animals ; Cell Death ; Hepatic Stellate Cells ; cytology ; Humans ; Liver Cirrhosis

Hepatic Stellate Cells ; Humans ; Liver Cirrhosis ; diagnosis ; metabolism ; Signal Transduction

Herpetone( HPT) is a bioactive lignan extracted from Herpetospermum pedunculosum,which can protect liver,lower aminotransferase and inhibit hepatitis B virus. However,HPT has a poor oral bioavailability due to its poor water solubility. And there is no report about whether HPT has an anti-hepatic fibrosis activity. To improve the dissolution of HPT and study its anti-hepatic fibrosis activity and mechanism,the study group prepared herpetone nanosuspensions( HPT-NS) by the miniaturized media milling method. The formulation and process of HPT-NS were optimized by the single factor experiment. Scanning electron microscopy was used to observe morphology of HPT-NS. Dialysis method was used to study dissolution of HPT-NS in vitro. CCK8 method was used to assess the effect of HPT-NS on proliferation of the rat hepatic stellate cells( HSC-T6). Flow cytometry was used to assess the effect of HPT-NS on apoptosis and cell cycle of HSC-T6. The mean particle size of optimized HPT-NS was( 196±7) nm with a polydispersity index of 0.279±0.009.SEM showed that HPT-NS was in a regular rod shape. The cumulative dissolution rate of HPT-NS reached 93% in 18 h,and was higher than that of herpetone coarse suspensions( HPT-CS,28%). CCK8 experiment showed that the inhibition rate of HPT-NS on HSC-T6 was higher than that of HPT-CS. Flow cytometry showed that HPT-NS could block HSC-T6 cells in G2/M phase and induce apoptosis of HSC-T6 cells,with a significantly stronger effect than HPT-CS. The results revealed that HPT-NS significantly increased the in vitro dissolution of HPT,and enhanced the inhibitive effect on HSC-T6 cell proliferation by blocking cells in the G2/M phase and inducing late apoptosis.
Animals ; Cell Line ; Hepatic Stellate Cells ; Lignans ; Liver Cirrhosis ; Rats