Search Results

3.Clinical implications of heterogeneity of hepatitis C virus.

Jun CHENG

Chinese Journal of Hepatology 2006;14(1):54-55

4.Investigation of the genotype distribution of hepatitis C virus among Turkish population in Turkey and various European countries.

Vedat TURHAN ; Nurittin ARDIC ; Can Polat EYIGUN ; Ismail Yasar AVCI ; Ali SENGUL ; Alaaddin PAHSA

Chinese Medical Journal 2005;118(16):1392-1394

5.Inhibitory effects of LNAzyme on expression of hepatitis C virus 5'-NCR Internal Ribosome Entry Site in HepG2.9706 cells.

Yi-bin DENG ; Le-gen NONG ; Yan-fei WNAG

Chinese Journal of Hepatology 2010;18(8):624-625

6.Construction and recovery of chimeric rabies virus expressing envelop proteins E1E2 of hepatitis C.

Jia-Liang DU ; Ying HUANG ; Qing TANG ; Li-Hua WANG ; Guo-Dong LIANG

Chinese Journal of Experimental and Clinical Virology 2011;25(3):170-172

OBJECTIVEConstruction and recovery of chimeric rabies virus expressing HCV envelop proteins E1E2.

METHODSOn the basis of the previously established reverse genetic system CTN-GFP, HCV E1E2 genes were cloned to both replication competent and replication constrained viral vectors based on CTN181 strain and the chimeric viruses CTN-HCV E1E2 and CTNdeltaG-HCV E1E2 were recovered.

RESULTSThe result demonstrated that both the chimeric viruses were rescued successfully, had the ability to re-infect normal sensitive cell lines and express HCV E1E2 genes detected in the level of mRNA.

CONCLUSIONThe establishment of chimeric RVs expressing HCV E1E2 genes provides the evidence that it is feasible to develop novel HCV vaccines based on viral vectors in theory and in practice.

Animals ; Cell Line ; Cricetinae ; Hepacivirus ; genetics ; metabolism ; Rabies virus ; genetics ; metabolism ; Viral Envelope Proteins ; genetics ; metabolism

7.Role of IL-28B SNPs in the treatment and prognosis of HCV infection.

Jian TAO ; Jun LIU ; Kun-long BEN

Chinese Journal of Hepatology 2011;19(4):316-317

8.Effects of HCV NS3 protein on apoptosis of QSG7701 cells induced by serum starvation.

Shu-yan SUN ; Hui GUO ; Bo LI ; Qiong-qiong HE ; De-yun FENG

Chinese Journal of Hepatology 2007;15(7):540-541

9.Subgenomic replicon model of hepatitis C virus and it's application.

Yin-Tang JIA ; Lai WEI

Chinese Journal of Hepatology 2005;13(2):158-160

10.Research advances on treatment of hepatitis C infection based on RNA interference and microRNA modulation.

Yan-ning LIU ; Guo-hua LOU ; Zhi CHEN

Journal of Zhejiang University. Medical sciences 2011;40(6):609-616

Infection with hepatitis C virus (HCV) is one of the major global health problems, approximately 170 million people are infected worldwide. The chronic HCV infection is associated with a high risk of developing liver cirrhosis, hepatocellular carcinoma (HCC) and liver failure. Unfortunately, there is still no effective vaccine or antibodies available for the prevention of infection. RNA interference (RNAi) represents a promising new approach to combat viral infections, and recent developments in the field of gene therapy have increased the feasibility of clinical applications. RNAi techniques have made rapid progress in the basic understanding of HCV biology and revealed numerous new viral and host-cell factors as potential targets for therapy. Together with the improvement of gene delivery technique and the discovery of the critical role of microRNA (miRNA) in HCV infection, RNAi and miRNA-based antiviral strategies hold great promise for the future. In this article, we provide a comprehensive overview of current developments of therapeutic targets of RNAi, liver-targeted delivery systems and the potential applications of miRNAs in treatment of hepatitis C infection.
Genetic Therapy ; Hepacivirus ; genetics ; Hepatitis C ; therapy ; Humans ; MicroRNAs ; genetics ; RNA Interference

1.Current research progress towards understanding the roles of hepatitis c-related microRNAs.

2.Advance of the natural intergenotypic recombination in hepatitis C virus.