Objective To explore the relationship between Helicobacter pylori (Hp) infection and psoriasis vulgaris, and investigate the effect of anti-Hp therapy on the patients with psoriasis vulgaris. Methods 13C-urea expiration test and detection of Hp antigen in feces samples were performed to identify Hp infection in 62 psoriasis patients and 35 healthy persons. Detection results of the two groups were statistically analyzed. The 62 psoriasis patients were divided into two groups based on the detection of Hp infection: Hp positive group (32 cases) and Hp negative group (30 cases). Patients in Hp positive group were given anti-Hp triple therapy (omeprazole, amoxicillin, clarithromycin) for two weeks and topical application of tretinoin for six weeks, while the patients in Hp negative group were treated only with topical use of tretinoin for six weeks. The total effective rate, score of psoriasis area, severity index (PASI) and visual analogue scale (VAS) of pruritus were recorded and then analyzed. Results The Hp infection rate in 62 psoriasis patients was 51.6%, and in healthy individuals was 22.9%, the difference between the two groups was statistically significant (P

Objective To study the expression and distribution of CB2 in rat skin tissue. Method Immunohistochemistrial technics were employed to study the expression and distribution of CB2 in rat skin tissue. Results CB 2 receptors mostly presented in suprabasal layers of the epidermis and hair follicles in dermis by CB2 like immunoreactivity with a N terminal anti CB2 receptor antibody. The positive expression of CB2 appeared in rat spleen and a negative result occured in liver. Conclusion The CB2 receptors distributed mainly in the rats, epidermis and hair follicles, may involve in some of the dermic physiological and pathological process, such as the communication between central neural system and skin.

ObjectiveTo evaluate the efficacy and safety of Qingpeng ointment in the treatment of chronic eczema.MethodsTotally,95 patients with chronic eczema were enrolled and divided into 3 groups to receive topical Qingpeng ointment (n =33),0.2％ hydrocortisone cream (n =32),10％ urea ointment (n =30) respectively twice a day for 4 weeks.All the patients were followed up once a week for the evaluation of therapeutic and side effects.ResultsAfter 4-week treatment,the response rate was 78.8％,84.4％ and 46.7％ in the Qingpeng ointment-,hydrocortisone cream- and urea ointment-treated patients respectively.Significant differences were observed in the response rate between the Qingpeng ointment- and urea ointment-treated patients (P ＜ 0.05 ),but not between the hydrocortisone cream- and Qingpeng ointment-treated patients (P ＞0.05).Skin flushing with a sensation of burning was observed at the application sites of 2 patients treated with Qingpeng ointment,and tingling sensation at the application sites was reported in 1 patient treated with hydrocortisone cream.All of these adverse reactions were tolerable and had no influence on the treatment.Conclusion Topical Qingpeng ointment is effective and safe for the treatment of chronic eczema.

Objective To investigate the expression and localization of dopamine- and cAMP-regulated phosphoprotein of 32 000 (DARPP-32) in mouse kidney tissue. Methods The cellular localization of DARPP-32 in mouse kidney tissue was detected by immunoblot and immunohistochemistry. Results DARPP-32-like immunoreactivity was detected in the medullary thick ascending limb of the loop of Henle, the cortical proximal convoluted tubule, and collecting ducts in medullary rays. The renal tubules were enriched of Na+, K+-ATPase for sodium reabsorption. Conclusion The participation of DARPP-32 is a likely crucial step of the signal-transduction pathway of dopamine regulation on sodium reabsorption in renal tubule cells.

This paper described the measures taken by Sir Run Run Shaw Hospital of Zhejiang University School of Medicine in development of general medicine,and differential care of acute and chronic diseases. Rational allocation of medical resources and leveraging of tertiary hospital′s advantages in medicine and education effectively improved the utilization ratio of medical resources for better health of the people.

OBJECTIVE To compare modified Kane/Fischer system and PCR method in identification for Trichophyton rubrum.METHODS Using modified Kane/Fischer system and TR1F and TR1R primers to identify 32 strains of dermatophytes.RESULTS The modified Kane/Fischer system and PCR could rapidly and stably(identify) T.rubrum.CONCLUSIONS The two methods can be used as the standard system to identify T.rubrum.

0.05);while great significances were found between group C and group B and A(P

Objective To investigate the expression of cannabinoid receptor 2 (CB2) in pigmented nevus and malignant melanoma and its significance. Methods The protein and mRNA expressions of CB2 were measured in tissue samples of 20 patients with pigmented nevus and 20 patients with malignant melanoma using immunohistochemical staining and RT-PCR, respectively. Results CB2 was expressed in both pigmented nevus and malignant melanoma. In tissue of pigmented nevus, CB2 was chiefly expressed in nevocytes and basal cell layer, and only a small quantity of CB2 was expressed in subcutaneous tissue. Statis-tical differences were observed between samples of malignant melanoma and pigmented nevus in the expres-sion intensity of CB2 (P < 0.05). Conclusions Increased protein and mRNA expressions of CB2 are observed in tissue of malignant melanoma, and the expression intensity of CB2 is higher in tissue of malig-nant melanoma than in that of pigmented nevus.

Objective To explore the management of nursing adverse events based on JCI evaluation criteria, and to establish a voluntary, non-punitive nursing adverse event reporting system.Methods From January 2014 to December 2015, 680 hospital nurses implemented JCI standards, revision of nursing adverse events management system, created online of nursing adverse events reporting system, to all nursing staff of adverse event management knowledge training and improvement before the analysis of the implementation of the JCI standard, adverse event reporting process and treatment measures of awareness, took the initiative to report rate, nursing adverse events incidence, patient satisfaction rate.Results Before and after nursing staff of adverse event reporting procedure and disposal method of awareness to improve the rate of 39.43% implementation, nursing staff to take the initiative to report to enhance the rate of 7.54%,the incidence of nursing adverse events to reduce the rate of 0.07%,the patients' satisfaction rate increased by 6.15%,there were statistically significant differences(x2=13.50,10.15,12.09,231.51,all P<0.05).Conclusion Nursing adverse events management constructed based on JCI accreditation standards is helpful to avoid the occurrence of nursing adverse events, to improve the rate of patients' satisfaction to nursing, to create a secure hospital atmosphere.

In China's new healthcare reform, the pilot local governments explore the practice of establishing a new model of hierarchical diagnosis and treatment system.Zhejiang Province has adopted a special policy of effectively allocating hospital resources and human resources, and efficiently improving primary healthcare institution capability and patient satisfaction(hereinafter referred to as double allocation, double improvement), focusing on the implementation of the 'Healthcare Talents Project', in order to fill a vacancy of human resources in primary healthcare institutions.This paper uses system dynamics modeling and the WISN method of WHO to estimate the gap in physician supply in primary healthcare institutions.After building the system dynamics model of 'Healthcare Talents Project', this paper simulates the influence of the policy on the vacancy of doctors in primary healthcare institutions and analyzes the sensitivity of regulatory factors.The simulation results show that, there are a big gap in physician supply of about 14,000 to build the hierarchical diagnosis and treatment system.The project can gradually increase the number of primary doctors, and the policy may fill the vacancy by 2021.However, if the efficiency of the hospital doctors who give assistance to primary institutions is increased by 10%, the targeted training and recruitment 100% achieve the policy plans and objectives, the project goal may be achieved by 2020.Therefore, this project can effectively adjust the human resources structure quickly and reasonably, and it can be used as reference for the reform of hierarchical diagnosis and treatment system.