To establish a new immune assay for Penicillic Acid (PA) from Penicillium cyclopium, we studied the synthesis of conjugated complete antigens for penicillic acid. PA was conjugated to bovine serum album (BSA) and ovalbumin (OVA) by 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide hydrochloride (EDC). The artificial antigens PA-BSA and PA-OVA were identified by ultraviolet spectrometric scanning, SDS-PAGE and immunization. Results showed that the absorption peak of conjugation were different from that of the carrier protein alone and of the PA. The conjugated ratio of PA and BSA was 23.2:1 and that of PA and OVA was 10.4:1. Balb/c mice were immunized by the artificial antigen of PA-BSA, with PA-OVA as coating antigen. The average titer of antiserums was more than 12 800 by indirect ELISA. The obtained antigens offered a basis for developing immunoassay method.
Animals ; Antibodies ; blood ; Antigens ; immunology ; metabolism ; Enzyme-Linked Immunosorbent Assay ; Ethyldimethylaminopropyl Carbodiimide ; analogs & derivatives ; chemistry ; Immunization ; Mice ; Mice, Inbred BALB C ; Ovalbumin ; immunology ; Penicillic Acid ; immunology ; metabolism ; Penicillium ; immunology ; metabolism ; Serum Albumin, Bovine ; immunology