Objectives: To investigate the expression and distribution of survivin and P53 in human non small cell lung cancer (NSCLC),and to evaluate the relationship between survivin and P53. Methods:The expression of survivin and P53 in 95 patients with NSCLC were examined using immunohistochemical methods (SP), and the relationship between them and clinicopathological parameters was analyzed. Results: The positive rates of survivin and P53 in NSCLC were 62% and 55.8% respectively, in the normal lung tissues nearby were 17% and 7% respectively( P

Objective:Clusterin is highly expressed in lymphoid and hematopoietoid tissue tumors in literature.This study is to investigate the significance of clusterin in the diagnosis of tumors by detecting its expression in follicular dendritic cell sarcoma/tumor(FDCS/T) and anaplastic large cell lymphoma(ALCL).Methods: The expression of clusterin was detected by immunohistochemical method in ALCL,diffuse large B-cell lymphoma(DLBCL),follicular lymphoma(FL),mantle cell lymphoma(MCL),NK/T-cell lymphoma(NK/T),peripheral T-cell lymphoma(PTL),plasma cell lymphoma(PCL),classical Hodgkin lymphoma(CHL) and FDCS/T.The expressions of CD30 and ALK in ALCL,and of CD21+CD35 in FDCS/T were also detected,and so were those of clusterin and CD21+CD35 in 10 cases of reactive hyperplasia of the lymph node were detected.Results: Clusterin and CD21+CD35 were both expressed in 8 cases of FDCS/T.The positive expression rates of clusterin,CD30 and ALK1 in 20 cases of ALCL were 85.00%(17/20),100%(20/20) and 50.00%(10/20),respectively.Clusterin was expressed in 4.70% of the 85 DLBCL cases(4/85),but not in other lymphoma tissues;clusterin and CD21+CD35 were both expressed in 100% of the 2 FDCS/T cases,and they were also expressed in reactive hyperplasia of lymph nodes as well as in follicular dendritic cells,but not in B-and T-cells.Conclusion: Clusterin might be a new marker for follicular dendritic cells,helpful to the diagnosis and differential diagnosis of ALCL.

Objective: To investigate the expression and distribution of NOS2 and NOS3 in human non-small cell lung cancer (NSCLC),and to evaluate the relationship between their expression and tumor angiogenesis and lymph node metastasis. Methods: the expression of NOS2, NOS3 and IMVD in 95 patients with NSCLC were examined using immunohistochemical methods (S-P), and the relationship between them and many clinicopathological parameters was analyzed. Results: The positive expression of NOS3 was associated with histological subtype, IMVD and lymph node metastases of NSCLC(P

Objectives:To investigate the expression and distribution of b FGF in human non small cell lung cancer (NSCLC),and to evaluate the relationship between the expression of b FGF and the biological behavior of NSCLC, especially tumor angiogenesis and lymph node metastasis. Methods:The expression of b FGF and IMVD in 95 patients with NSCLC was examined using immunohistochemical methods (SP), and the relationship between the expression and many clinicopathological parameters were analyzed. Results: The positive rates of b FGF in carcinoma tissue (NSCLC) were much higher than those in the normal lung tissues nearby ( P 0.05). Conclusions: b FGF may play an important role in tumor angiogenesis and lymphatic metastasis of human NSCLC , and detection of b FGF may be a good metastasis and prognostic predictors for human NSCLC.

Purpose To investigate the expression of EphA1 in renal cell carcinomas ( RCC) and to analyze its correlation with clini-copathological parameters in order to explore the clinical significance of EphA1 protein in renal cell carcinomas ( RCC) . Methods The immunocytochemical method was employed to measure the expression of EphA1 in 144 clear cell RCC ( ccRCC) tissues, 18 chro-mophobe RCC tissues and 6 papillary RCC tissues. Correlation between EphA1 protein expression and clinical parameters was evaluated by statistics. Results High level of the expression of EphA1 was observed in all normal renal tubes. The EphA1 protein was negative-ly or weakly expressed in 93 out of 144 ccRCC (64. 6%) and positively expressed in 51 out of 144 ccRCC (35. 4%). Positive expres-sion of EphA1 was observed in all samples of chromophobe RCC and papillary RCC. The high level expression of the EphA1 protein was significantly associated with younger patients (P<0. 001), sex (P=0. 016) and lower nuclear grade (P<0. 001). No significant relation between the expression of EphA1 and tumor diameter was found ( P=0. 316 ) . Conclusion EphA1 protein may be a new marker for the prognosis of ccRCC.

Objective To detect the expression and distribution of activated cytotoxic cells in types of lymphoma with tissue microarray, and provide evidences for clinical treatment and prognosis. Methods Immunohistochemical staining by S-P technique was used to detect the expression and distribution of perforin and granzyme B in the lymphoma tissue microarray, composed of 60 cases of lymphoma tissue. Ten cases of NK/T-cell lymphoma routine sections were used for relative research, and ten cases of reactive hyperplasia were used for comparison. Results In the tissue microarray, originated from intranode and extranode were 48 cases and 12 cases, respectively; consisting of 42 cases of B-cell lymphoma, 16 cases of T-cell lymphoma, two cases of Hodgkin's disease. 42 cases of B-cell lymphoma cells were negative in perforin and granzyme B. In Ten cases of peripheral T-cell lymphoma, perforin and granzyme B positive were eight cases and nine cases, respectively, but the positive cells were no tumor cells. In 12 cases of NK/T-cell lymphoma (two in the tissue microarray, ten routine sections), both perforin and granzyme B were strongly positive. B-cell lymphoma, T-cell lymphoma and NK/T-cell lymphoma differed significantly (P<0.01). Conclusion Perforin and granzyme B were immunity markers for the identification of activated cytotoxic cells, which also could be used as diagnostic markers of NK/T-cell lymphoma. Their expression in B-cell lymphoma reflected the anti-tumor immunologic reaction of host. Tissue microarray technology has the behavior of high-throughput, convenient, effective, small experiment error, good replication and so on, and can be used as a useful tool for research of lymphoma.

ObjectiveTo investigate the continuous pathological features of biopsy specimens from five cases of small bowel allotransplantation (SBT) in order to provide more reliable information for the diagnosis and treatment of acute rejection (AR) in SBT.Methods324 biopsy specimens of intestinal mucosa after SBT from 5 patients were collected and studied by histology,histochemistry and electron microscopy.ResultsIn the early stage after operation (0～3 months),AR IND-1 grade was diagnosed for four times on 3 of 5 patients.During 3-6 months,AR IND-1 grade for three times was diagnosed in 2 cases,and AR 2 grade for two times during 7 ～ 12 months. All the patients suffered ischemia reperfusion injury, lymphatic vessel reconstruction and AR.Conclusion The pathological examination of biopsy specimens of intestinal mucosa is still the most reliable detecting method to diagnose AR,and continuous observation may play an important role to monitor the occurrence,development,and treatment response of AR. The final diagnosis of AR depends on structure of intestinal mucosa,crypt epithelium injury and inflammatory cells infiltration. The communication among the pathologist and surgeon is the best way to reduce misdiagnoses.Ultrastructural examination is used to verify the pathogenic microorganism.

Purpose To investigate the clinicopathologic features, diagnosis and differential diagnosis of ovarian juvenile granulosa cell tumor (JGCT).Methods The history records, pathologic features and immunophenotype of 8 cases of JGCT were retrospectively evaluated and their prognosis was achieved by follow-up.Results The age of patients ranged from 6～21 years old,with an average age of 15.1 years.The main clinical manifestations included an abdominal mass, ascites and isosexual pseudoprecocity. Cut surface of the tumor was typically solid with cysts formed. The histopathological changes displayed solid nests, diffuse sheet, multiple round or ovoid follicles in variable size.Macrofollicles could be seen in some cases.The follicular pattern consisted of small cystic cavities containing eosinophilic secretions. The tumor cells were round or polygonal, medium in size. The tumor cells had abundant pale or slightly eosinophilic cytoplasm, round nuclei with fine chromatin. Nuclear grooves were inconspicuous.Mitosis figures could be found. Immunohistochemical results showed that the tumor cells expressed inhibin-α,CD99,vimentin; while Melan-A,calretinin and S-100 were positive staining in part of the cases.CKpan,EMA,PLAP,Syn and CgA were negative in all the cases.Conclusions Ovarian juvenile granulosa cell tumor is a rather rare, low malignant tumor with good prognosis. Its diagnosis depends on the histologic and immunohistochemical findings and clinical features. Its differential diagnosis includes adult granulose cell tumor, hypercalcaemic type small cell carcinoma, carcinoid and dysgerminoma.

Objectives:To study the clinicopathological features of extraovarian peritoneal serous papillary carcinoma.Methods:The clinical pictures and pathological characteristics of 6 cases extraovarian peritoneal serous papillary carcinoma admitted to our hospital from 1992 to 1999 were studies retrospectively.H & E staining,histochemical staining and immunohistochemical staining were used.Results:The ages of the 6 patients were between 32～62(43.7) years old.The bilateral ovaries and fallopian tubes were almost normal.Tumors were examined histomorphologically by microscope and electron microscope,periodic acid-Schiff (PAS) stain was used in the histochemical study and carcinoembryonic antigen (CEA),CA125,p53,AE1 and AE3 monoclonal antibody assays were detected immunohistochemically.The results revealed no difference between extraovarian peritoneal serous papillary carcinoma and ovarian serous papillary carcinoma.Conclusions:Extraovarian peritoneal serous papillary carcinoma is considered arising from the secondary Müllerian system.Histologically,extraovarian peritoneal serous papillary carcinoma was identical to that of the similar epithelial carcinomas arising from the ovaries.The diagnosis was made only when the ovaries were not involved and without any evidence of similar epithelial carcinoma obtained in the ovaries.Histochemical staining and immunohistochemical staining are helpful as accessory criteria for the differential diagnosis between primary carcinoma of the peritoneum and malignant mesothelioma.The prognosis is poor.

Objective: To improve the limitation and stability of tissue processing on mucosa from small intestine transplantation and to provide better evidence of pathological diagnosis for the acute rejection on small intestine transplantation. Method:92 samples of mucosa from small intestine transplantation were reviewed.There were three methods of tissue processing (ultrasonic wave and microwave as well as routine) were adopted.The results were analyzed with statistical methods. Results: Among 18 samples processed by the method of ultrasonic wave,4 samples were A grade (22.22%). Among 50 samples processed by the method of microwave,30 samples were A grade (60.00%). Among 24 samples processed by the method of routine,13 samples were A grade (54.17%).The Chi Square Test suggested that there was statistic difference among three processing methods. Conclusions: Microwave is the best method of tissue processing on mucosa of small intestine transplantation and for the diagnosis acute rejection.