Pollution and control of microcystins released during the toxic bloom was reviewed in the present paper. As a risk factor in safety assessment, microcystins aroused broad attention. It was a topic of general interest that how to control microcystins reproduction and how to enhance the degradation of microcystins in the water treatment procedure. Based on the state of China, it was considered that a combined method of biological, physical and chemical treatment should be the best way to control microcystins pollution in drinking water.

In order to establish a method by which the recombinant suicide plasmids integrated on the chromosome could be recircled, A simple method of transconjugative cloning was established with the helper plasmids pMT999 or pRK2013 and fusion strains of Shigella flexneri which were obtained by screening with in vivo expression technology. And the cloning efficiency with this method is very high.

Drug Resistance, Bacterial ; Gram-Positive Bacteria ; isolation & purification ; Humans ; Infant, Newborn ; Microbial Sensitivity Tests ; Sepsis ; diagnosis ; drug therapy ; microbiology

OBJECTIVETo investigate the expression of calcitonin receptor mRNA in the osteoclasts of the resorbing deciduous teeth.

METHODSAfter fixing the collected deciduous teeth, toluidine blue was performed and tartrate-resistant acid phosphatase (TRAP) staining was used to identify the osteoclasts on the resorbing surface of human deciduous teeth and in situ hybridization of calcitonin receptor mRNA to show its existence.

RESULTSThere were a number of TRAP positive osteoclasts on the root surface which showed the expression of calcitonin receptor mRNA.

CONCLUSIONOn the resorbing surface of human deciduous teeth there are osteoclasts that express calcitonin receptor mRNA, so it is feasible to use this kind of osteoclast to test the effect of external factors on the expression of CTR mRNA.

Humans ; In Situ Hybridization ; In Vitro Techniques ; Osteoclasts ; metabolism ; RNA, Messenger ; biosynthesis ; Receptors, Calcitonin ; biosynthesis ; genetics ; Tooth, Deciduous ; cytology ; metabolism

OBJECTIVEVentilator-associated pneumonia (VAP) is a common nosocomial infection and is responsible for a very high mortality in neonatal intensive care unit (NICU) patients. This study was designed to investigate the etiology and high risk factors of neonatal VAP.

METHODSThe clinical data of 106 critical neonates who were treated with mechanical ventilator between 2003 and 2005 were studied retrospectively.

RESULTSOf the 106 neonates, 84 received mechanical ventilation for > or = 48 hrs. Thirty-five (41.7%) out of the 84 patients developed VAP. Univariate analysis showed that gestational age, duration of mechanical ventilation, reintubation, birth weights, primary lung disease and gamma globulin administration were associated with the development of VAP (P < 0.05). Multivariate stepwise logistic regression analysis showed that primary lung disease (OR=3.671, 95% CI=1.0-13.45, P < 0.05), duration of mechanical ventilation (OR=4.945, CI=1.51-16.21, P < 0.01), reintubation (OR=7.721, 95% CI=2.31-25.85, P < 0.01) and high-dose gamma globulin administration (OR=5.520, 95%CI=2.08-16.26, P < 0.01) were predicted factors for the development of VAP. The detection rate of gram negative bacilli (76.9%) was the highest, followed by gram positive coccus (17.9%) in VAP patients.

CONCLUSIONSOpportunistic drug-resistant bacteria are common pathogens for neonatal VAP. The risk of VAP is multifactorial, including external medical environments and patients' internal agents.

Anti-Bacterial Agents ; therapeutic use ; Female ; Humans ; Infant, Newborn ; Logistic Models ; Male ; Microbial Sensitivity Tests ; Pneumonia, Ventilator-Associated ; drug therapy ; etiology ; Retrospective Studies ; Risk Factors

In order to find the compound basis of Phlomis younghusbandii Mukerjee that related to pharmacodynamic action, various chromatographic techniques were used to separate and purify the constituents of this plant, and physicochemical and spectral data were used to identify the structures of obtained compounds. A new furanolabdane diterpene glycoside, named as phlomisoside F, was isolated and identified, which was 15,16-epoxy-8(9),13(16), 14-labdatrien-7-ketone-19-oic acid-beta-D-glucopyranosyl ester.
Diterpenes ; chemistry ; isolation & purification ; Glucosides ; chemistry ; isolation & purification ; Glycosides ; chemistry ; isolation & purification ; Molecular Structure ; Phlomis ; chemistry ; Plant Tubers ; chemistry ; Plants, Medicinal ; chemistry

BACKGROUNDDiffusion-weighted imaging has been widely used to differentiate the character of lymphadenopathy. But there are significant differences between prior studies. The aim of the study was to compare the benefit of apparent diffusion coefficient (ADC) and rADC in the differentiation of metastatic and benign lymph nodes in a rabbit model.

METHODSTwo observers independently measured ADCs in quadriceps of every rabbit on diffusion-weighted images with different sizes of regions of interest (ROI). The appropriate ROI for rADC was determined using the interobserver coefficient. rADC was calculated by ADC(lesion)/ADC(reference site). Receiver operating characteristics (ROC) analysis was performed to compare the diagnostic value of the ADC values and rADC values in differentiating metastatic from benign lymph nodes.

RESULTSWhen the ROIs included five pixels, the ICC was 0.816, indicating a good interobserver agreement. The differences of ADC and rADC values between metastatic and benign lymph nodes were both statistically significant. The area under the ROC curve was greater for the rADC than for the ADC. With the rADC criteria of 0.640, the sensitivity and specificity for differentiating metastatic from benign lymph nodes were 93% and 90%, respectively.

CONCLUSIONAs a reference site, muscles reveal a good repeatability using a small ROI and the rADC may improve diagnostic accuracy for detecting metastatic nodes in animal models.

Animals ; Diffusion Magnetic Resonance Imaging ; methods ; Lymph Nodes ; pathology ; Lymphatic Metastasis ; diagnosis ; Rabbits ; Sensitivity and Specificity

Coal ; Dust ; analysis ; Gases ; adverse effects ; Humans ; Male ; Middle Aged ; Occupational Exposure ; analysis ; Pulmonary Alveolar Proteinosis ; etiology

Objective To explore the treatment for endometriosis(EMs)by Professor SITU Yi,a Guangdong famous traditional Chinese medicine physician,thus to provide reference for the diagnosis and treatment of EMs in traditional Chinese medicine based on personalized knowledge graph analysis and data mining technology.Methods Based on the literature research achievements of diagnosis and treatment of EMs,the ontology knowledge tree of famous physician's principles,methods,prescriptions and medicines was established by user-computer integration method.The medical records of EMs patients treated by Professor SITU Yi in the outpatient department of Guangdong Provincial Hospital of Chinese Medicine from 2013 to 2019 were collected.After standardization of the data of medical records,the knowledge set was defined,the knowledge map of TCM diagnosis and treatment of EMs was constructed,and the symptom characteristics and medication rules for EMs were analyzed.Results The core symptoms of EMs patients were dysmenorrhea,chronic pelvic pain and infertility,with the predominated syndrome of blood stasis.The pathogenesis of EMs patients was mainly due to qi stagnation,kidney deficiency,and qi deficiency.Frequent itemset analysis yielded the core drug groups of"Corydalis Rhizoma,Cyperi Rhizoma,Paeoniae Radix Alba""Lycii Fructus,Taxilli Herba,Poria,Dioscoreae Rhizoma"and"Aurantii Fructus,Leonuri Herba".The EMs word cloud map constructed on the basis of knowledge element clustering and neural network algorithm showed that the drug combinations of"Salviae Miltiorrhizae Radix et Rhizoma,Paeoniae Radix Rubra""Corydalis Rhizoma,Curcumae Radix""Cinnamomi Ramulus,Moutan Cortex,Paeoniae Radix Rubra,Poria""Salviae Miltiorrhizae Radix et Rhizoma,Paeoniae Radix Rubra,Sparganii Rhizoma,Curcumae Rhizoma""Chuanxiong Rhizoma,Corydalis Rhizoma"and"Taxilli Herba,Cuscutae Semen"were commonly used for the treatment of EMs by Professor SITU Yi.The results of hierarchical clustering analysis and association network construction for Chinese medicinals,symptoms and syndromes showed that there were complex inner link among the syndromes,symptoms and medication.Conclusion For the treatment of EMs,Professor SITU Yi usually adopt the methods of activating blood to remove stasis and tonifying kidney,and also performs the periodic therapy and simultaneous application of purging and nourishing therapeutics.Knowledge graph analysis and data mining based on semantic web are helpful to unveil the tacit knowledge of clinical diagnosis and treatment experience of Professor SITU Yi,which can provide reference for the differentiation and treatment of EMs with Chinese medicine.

OBJECTIVETo evaluate the masticatory efficiency of implant supported dentures in partially edentulous patients and the patients' satisfaction on masticatory function.

METHODSThe masticatory efficiency of implant supported dentures of 22 patients were tested. The questionnaire of the patients' satisfaction about masticatory function had also been collected. The correlativity of the masticatory efficiency of implant supported dentures and the scores evaluated by patients on masticatory function were analyzed.

RESULTSThere were no differences in masticatory efficiency between implant supported denture and non-implant supported denture (natural teeth and porcelain-fused-to-metal fixed bridges). The patients gave high scores to the satisfaction about masticatory function in the questionnaire. But the test results of masticatory efficiency were not related with the scores evaluated by patients.

CONCLUSIONThe implant supported denture could meet the requirement of normal masticatory function. The patients were satisfactory with the masticatory function of implant supported dentures, but the patients' subjective evaluation about masticatory function was probably influenced by varied factors.

Anodontia ; Dental Prosthesis, Implant-Supported ; Female ; Humans ; Male ; Mastication ; Middle Aged ; Mouth, Edentulous ; Patient Satisfaction