Pollution and control of microcystins released during the toxic bloom was reviewed in the present paper. As a risk factor in safety assessment, microcystins aroused broad attention. It was a topic of general interest that how to control microcystins reproduction and how to enhance the degradation of microcystins in the water treatment procedure. Based on the state of China, it was considered that a combined method of biological, physical and chemical treatment should be the best way to control microcystins pollution in drinking water.

In order to establish a method by which the recombinant suicide plasmids integrated on the chromosome could be recircled, A simple method of transconjugative cloning was established with the helper plasmids pMT999 or pRK2013 and fusion strains of Shigella flexneri which were obtained by screening with in vivo expression technology. And the cloning efficiency with this method is very high.

In order to find the compound basis of Phlomis younghusbandii Mukerjee that related to pharmacodynamic action, various chromatographic techniques were used to separate and purify the constituents of this plant, and physicochemical and spectral data were used to identify the structures of obtained compounds. A new furanolabdane diterpene glycoside, named as phlomisoside F, was isolated and identified, which was 15,16-epoxy-8(9),13(16), 14-labdatrien-7-ketone-19-oic acid-beta-D-glucopyranosyl ester.
Diterpenes ; chemistry ; isolation & purification ; Glucosides ; chemistry ; isolation & purification ; Glycosides ; chemistry ; isolation & purification ; Molecular Structure ; Phlomis ; chemistry ; Plant Tubers ; chemistry ; Plants, Medicinal ; chemistry

OBJECTIVETo investigate the expression of calcitonin receptor mRNA in the osteoclasts of the resorbing deciduous teeth.

METHODSAfter fixing the collected deciduous teeth, toluidine blue was performed and tartrate-resistant acid phosphatase (TRAP) staining was used to identify the osteoclasts on the resorbing surface of human deciduous teeth and in situ hybridization of calcitonin receptor mRNA to show its existence.

RESULTSThere were a number of TRAP positive osteoclasts on the root surface which showed the expression of calcitonin receptor mRNA.

CONCLUSIONOn the resorbing surface of human deciduous teeth there are osteoclasts that express calcitonin receptor mRNA, so it is feasible to use this kind of osteoclast to test the effect of external factors on the expression of CTR mRNA.

Humans ; In Situ Hybridization ; In Vitro Techniques ; Osteoclasts ; metabolism ; RNA, Messenger ; biosynthesis ; Receptors, Calcitonin ; biosynthesis ; genetics ; Tooth, Deciduous ; cytology ; metabolism

Coal ; Dust ; analysis ; Gases ; adverse effects ; Humans ; Male ; Middle Aged ; Occupational Exposure ; analysis ; Pulmonary Alveolar Proteinosis ; etiology

BACKGROUNDDiffusion-weighted imaging has been widely used to differentiate the character of lymphadenopathy. But there are significant differences between prior studies. The aim of the study was to compare the benefit of apparent diffusion coefficient (ADC) and rADC in the differentiation of metastatic and benign lymph nodes in a rabbit model.

METHODSTwo observers independently measured ADCs in quadriceps of every rabbit on diffusion-weighted images with different sizes of regions of interest (ROI). The appropriate ROI for rADC was determined using the interobserver coefficient. rADC was calculated by ADC(lesion)/ADC(reference site). Receiver operating characteristics (ROC) analysis was performed to compare the diagnostic value of the ADC values and rADC values in differentiating metastatic from benign lymph nodes.

RESULTSWhen the ROIs included five pixels, the ICC was 0.816, indicating a good interobserver agreement. The differences of ADC and rADC values between metastatic and benign lymph nodes were both statistically significant. The area under the ROC curve was greater for the rADC than for the ADC. With the rADC criteria of 0.640, the sensitivity and specificity for differentiating metastatic from benign lymph nodes were 93% and 90%, respectively.

CONCLUSIONAs a reference site, muscles reveal a good repeatability using a small ROI and the rADC may improve diagnostic accuracy for detecting metastatic nodes in animal models.

Animals ; Diffusion Magnetic Resonance Imaging ; methods ; Lymph Nodes ; pathology ; Lymphatic Metastasis ; diagnosis ; Rabbits ; Sensitivity and Specificity

OBJECTIVEVentilator-associated pneumonia (VAP) is a common nosocomial infection and is responsible for a very high mortality in neonatal intensive care unit (NICU) patients. This study was designed to investigate the etiology and high risk factors of neonatal VAP.

METHODSThe clinical data of 106 critical neonates who were treated with mechanical ventilator between 2003 and 2005 were studied retrospectively.

RESULTSOf the 106 neonates, 84 received mechanical ventilation for > or = 48 hrs. Thirty-five (41.7%) out of the 84 patients developed VAP. Univariate analysis showed that gestational age, duration of mechanical ventilation, reintubation, birth weights, primary lung disease and gamma globulin administration were associated with the development of VAP (P < 0.05). Multivariate stepwise logistic regression analysis showed that primary lung disease (OR=3.671, 95% CI=1.0-13.45, P < 0.05), duration of mechanical ventilation (OR=4.945, CI=1.51-16.21, P < 0.01), reintubation (OR=7.721, 95% CI=2.31-25.85, P < 0.01) and high-dose gamma globulin administration (OR=5.520, 95%CI=2.08-16.26, P < 0.01) were predicted factors for the development of VAP. The detection rate of gram negative bacilli (76.9%) was the highest, followed by gram positive coccus (17.9%) in VAP patients.

CONCLUSIONSOpportunistic drug-resistant bacteria are common pathogens for neonatal VAP. The risk of VAP is multifactorial, including external medical environments and patients' internal agents.

Anti-Bacterial Agents ; therapeutic use ; Female ; Humans ; Infant, Newborn ; Logistic Models ; Male ; Microbial Sensitivity Tests ; Pneumonia, Ventilator-Associated ; drug therapy ; etiology ; Retrospective Studies ; Risk Factors

Drug Resistance, Bacterial ; Gram-Positive Bacteria ; isolation & purification ; Humans ; Infant, Newborn ; Microbial Sensitivity Tests ; Sepsis ; diagnosis ; drug therapy ; microbiology

OBJECTIVETo evaluate the masticatory efficiency of implant supported dentures in partially edentulous patients and the patients' satisfaction on masticatory function.

METHODSThe masticatory efficiency of implant supported dentures of 22 patients were tested. The questionnaire of the patients' satisfaction about masticatory function had also been collected. The correlativity of the masticatory efficiency of implant supported dentures and the scores evaluated by patients on masticatory function were analyzed.

RESULTSThere were no differences in masticatory efficiency between implant supported denture and non-implant supported denture (natural teeth and porcelain-fused-to-metal fixed bridges). The patients gave high scores to the satisfaction about masticatory function in the questionnaire. But the test results of masticatory efficiency were not related with the scores evaluated by patients.

CONCLUSIONThe implant supported denture could meet the requirement of normal masticatory function. The patients were satisfactory with the masticatory function of implant supported dentures, but the patients' subjective evaluation about masticatory function was probably influenced by varied factors.

Anodontia ; Dental Prosthesis, Implant-Supported ; Female ; Humans ; Male ; Mastication ; Middle Aged ; Mouth, Edentulous ; Patient Satisfaction

Objective To compare the epidemiological characteristics of malaria before and after the elimination in Hangzhou, and to provide scientific basis for formulating malaria control strategies. Methods We collected the data of confirmed malaria cases in Hangzhou from 2005 to 2016. We compared the differences in epidemiological characteristics (demographic features of malaria cases, source of infection, plasmodium species, etc.) and vectors of malaria before (2005-2010) and after (2011-2016) the elimination of malaria. Results Totally 340 malaria cases were reported before the elimination in Hangzhou with the annual incidence rate of 0.16/10 million, while 227 cases were reported after the elimination with the annual incidence rate of 0.25/10 million. Local cases accounted for 11.47% before the elimination, while no local cases but all imported cases were reported after the elimination. Compared the sex, occupation and age of malaria cases, epidemic season, report area, source of infection and species before and after the elimination, the proportion of men increased from 67.65% to 89.87%; the proportion of 18-60 years old group increased from 75.88% to 96.04%; the proportion of children / students decreased from 17.35% to 7.49%;the epidemic season changed from July to October to no obvious season; the main report area changed from suburb (55.88%) to urban area (81.06%); the source of infection changed from the domestic provinces (77.94%) to foreign countries (99.12%) ;the main species of malaria parasites changed from vivax malaria (88.24%) to ciparum malaria (71.81%) . The Anopheles mosquitoes caught by vector monitoring were Anopheles sinensis. The average density of Anopheles sinensis before and after the elimination was 10.0 and 5.0 /(person·night) . Conclusion After the elimination of malaria in Hangzhou, imported malaria cases from abroad were dominant. and the men and women who returned to and from abroad and 18-60 years old were the key prevention and control groups in the future.The epidemiological characteristics of malaria after the elimination in Hangzhou have changed greatly. The men who are 18-60 years old and return from abroad are the focus of malaria prevention and control.