Background Previous study on critical period plasticity in local cortical circuits primarily was only to test the role of some proteins in visual cortex on visual development based on the existed neural signals expression system,but whole containing proteins analysis in cortical circuits is lack.To perform a whole containing proteins analysis has an important significance for critical period plasticity study.Objective This study was to investigate the influence of dark rearing on visual sense and proteomics in visual cortex for growing rat.Methods Two SD female rats were fed in two cages together with 12 newborn rats on the same day respectively,and half number of newborn rats were exchanged each other from first day after delivering and marked by eartipping.The newborn rats in a cage were bred in the dark environment for 40 days,and newborn rats in other cage were bred in the nature environment as controls.The blink response of rats to nearby object was examined and compared between the two groups of rats.Then three rats from two cages were sacrificed respectively and bilateral primary visual cortex tissue was isolated.Proteomics in rat primary visual cortex was detected by two-dimensional electrophoresis and mass spectrometry and the result was checked from database.Then the survival rats in the dark environment returned to the nature environment for 10 days,and the blink response of rats to nearby object were compared with that of agematched rats in the natural environment.The use and care of experimental rats followed the instruction of Ethic Committee of Nankai University.Results The blink response of rats was (0.33 ± 0.35) times in the dark environment for 40-day group,and that in the natural environment for 40-day group was (6.42±0.68) times,with a significant difference between them (t =24.38,P＜0.01).After returned to natural environment for 10 days,the blink response times of rats were less than those of the natural environment group ([5.00±1.22] times vs.[6.11±0.59]times),but this change was not statistically significant (t =2.09,P＞0.05).Two-dimensional electrophoresis and mass spectrometry assay revealed that 36 different proteins in visual cortex were found in the dark-feed rats compared with the natural environment rats,including 26 loss proteins and 10 extra proteins.Among the different proteins,Eps 15 homology domain-containing protein-3 (EHD3),tubulin alpha-1A chain and 2 ',3 '-cyclic-nucleotide 3 ' phosphordiesterase were the known proteins.Conclusions Dark rearing cause reversible visual loss in critical period plasticity newborn rat,and the change of proteomics in visual cortex is probably an affecting factor.

Objectives: To investigate the relationships between the stability of carotid plaque and serum Lp-PLA2, A-FABP levels in hypertensive postmenopausal women. Methods: 195 postmenopausal women with hypertension were selected and divided into non-plaque group, stable plaque group and unstable plaque group according to the results of carotid intima-media thickness (IMT) and plaque types derived from color doppler ultrasonography. In addition, 40 healthy postmenopausal women were recruited as normal control group. The serum Lp-PLA2 and A-FABP levels of all subjects were measured. Lp-PLA2 and A-FABP levels were compared among four groups by One-Way ANOVA. Spearman correlation analysis and multiple logistic regression analysis were also performed. Results: Plaque group included 123 subjects (unstable plaque group: 29 cases; stable plaque group: 94 cases), and non-plaque group included 72 subjects. The average serum A-FABP level was significantly higher in unstable plaque group [(172.60±33.70) ng/L] than in non-plaque group[(133.04±29.49) ng/L], P<0.05. Serum Lp-PLA2 level was similar between the four groups, P>0.05. Serum A-FABP level was positively correlated with the carotid plaque (r=0.3446, P=0.0049);serum Lp-PLA2 level was not correlated with the carotid plaque (r=0.2058, P=0.0996). Multiple logistic regression analysis showed that high A-FABP level was associated with stable plaque in hypertensive postmenopausal women (P=0.040, OR=1.017, 95%CI: 1.001~1.033), which was also associated with unstable plaque in this population (P=0.003, OR=1.031, 95%CI: 1.010~1.052). Conclusions: The level of A-FABP is a determinant responsible for the occurrence and stability of carotid plaque among hypertensive postmenopausal women. There was no correlation between Lp-PLA2 level and the stability of carotid plaque in this patient cohort.

There are definite effects of sunitinib molecular target therapy on renal carcinoma , but not all the patients diagnosed with renal carcino-ma could benefit from it.Single nucleotide polymorphism ( SNP ) could be related to point mutations of patients who couldn ’ t bear the toxicity that induced by sunitinib.Besides, the sunitinib induced toxicity could be influenced by the exposure of sunitinib in patient plasma .Therefore the aim of this article was to conduct a systematic review to the relation-ship among adverse reaction , SNPs and plasma concentration in renal carcinoma patients treated with sunitinib .

UNLABELLED: OBJECTIVE To explore the advantage and feasibility of fluorescent antibody method for detection of blood type in biological material. METHODS: According to theory of specific binding of antigen and antibody, at first the anti-A monoclonal antibody (MA) and anti-B MA were labeled with the fluorescent, then fluorescent-labeled antibodies (FLA) were bound with corresponding biological material (such as bloodstain) in the optimum condition, finally the ABO blood type of bloodstain was determined under microscope fluorescent. RESULTS: The fluorescent antibody method is highly sensitive, accurate and simple. CONCLUSION The fluorescent antibody method is an accurate and reliable method for detection of ABO blood type in biological material.
ABO Blood-Group System/immunology* ; Antibodies, Monoclonal/blood* ; Antigen-Antibody Reactions ; Blood Group Antigens/blood* ; Blood Stains ; Fluorescent Antibody Technique/methods* ; Forensic Medicine/methods* ; Humans ; Sensitivity and Specificity

Objective To investigate the pharmacokinetics of olopata-dine hydrochloride tablets in healthy volunteers. Methods Twelve healthy volunteers were divided into receiving orally a single dose of 5, 10,20 mg olopatadine hydrochloride tablets respectively. The plasma and urinary samples were determined by HPLC-MS/MS. Results The main pharmaeokinetic parameters of three different dosages (5,10,20 mg) were as follows: C_(max) were (76.73±26.61) , (133.61±61.63) and (270.44±115.19) μg·L~(-1); t_(max) were (0.75±0.19), (0.96± 0. 29) and (0. 83±0. 32) h; t_(1/2) were (5.20±2. 47), (6. 82±3.04) and (6.87±3.06) h; AUC_(0-t) were (246.70±66.07), (439. 19± 185.03) and (904.69±300.21) μg·h·L~(-1) and AUC_(0-∞) were (248.36±65.81), (442. 02 ± 186. 46) and (907.49±299. 92) μg·h·L~(-1). Mean of excretory rate of three different dosages (5, 10, 20 mg) was 54. 6%. Conclusion The value of C_(max) and AUC will increase following the increase of the doses. The pharmacokinetic characteristic of olopatadine hydrochloride in Chinese healthy vohmteers were fitted with linear kinetic model. It is showed that the sex-related differences did not exist in the main pharmacokinetic parameters between males and females by analysis of variance.

Objective To evaluate the bioequivalent between the two kinds of glipizide tablets.Methods With two-cycle crossover trial was design, 24 healthy male subjects were given a single oral dose 5 mg of glipizide test preparation and reference preparation.Serum samples were collected at different time points before administration and after adminis-tration.The plasma concentrations were measured by HPLC -MS/MS method.The pharmacokinetic parameters and relative bioavailability were calculated by WinNonlin 6.1 software.Results The main pharmacoki-netic parameters of the test and reference glipizide tablets were as fol-lows: tmax were ( 3.40 ±1.54 ) and ( 3.71 ±1.30 ) h, Cmax were (471.88 ±108.10 ) and ( 480.58 ±132.63 ) μg · L-1 , t1/2 were (5.15 ±1.31) and(5.32 ±1.27)h, AUC0-t were (2805.71 ±592.61 ) and (2873.40 ±697.57 )μg· h-1 · L, respectively.F0-t and F0-∞ of test preparation were ( 105.77 ±27.84 )% and ( 105.28 ±27.63 )% re-spectively.Conclusion The glipizide tablets test and reference are bio-equivalent.

Objective To establish a high performance liquid chroma-tography-tandam mass spectrometry ( HPLC-MS/MS) method for de-termining the concentration of sunitinib and N -desethyl sunitinib (SU12662) in human plasma.Methods Sunitinib, SU12662 and in-ternal standard sunitinib -D10 were extracted from plasma with one -step protein precipitation and the stereoselective analysis of sunitinib and SU12662 was achieved on an Agilent ZORBAX Extend C18 (2.1 mm ×75 mm ×3.5 μm) with gradient elution by a mobile phase consisting of wa-ter containing 0.1%formic acid and 95%acetonitrile.Electrospray ioni-zation source was applied, and multiple reaction monitoring mode was operated in the positive mode with the monitor ions at m/z 399.3→326.1 for sunitinib, m/z 371.3→283.1 for SU12662 and 409.3→326.2 for sunitinib-D10.Results The calibration curve for plasma sunitinib was linear in the range of 0.5-200 ng? mL-1 ( r=0.998 7) , the lower limi-tation of quantification was 0.5 ng? mL-1 , while the accuracy was ranged from 0.57% to 2.87%, the average recovery rate was ranged from 92.75%to 98.62%,and intra-and inter-day RSD were ranged from 0.91%to 1.92%and 5.40%to 7.87%, respectively.The calibration curve for plasma SU12662 was linear in the range of 0.25 -100 ng? mL-1 ( r =0.999 9 ) , while the accuracy was ranged from -2.08% to 2.80%, the average recovery rate was ranged from 96.23%to 101.12%, and intra-and inter-day RSD were ranged from 0.46%to 2.46%and 5.84%to 9.75%, respectively, the lower limitation of quantification was 0.25 ng? mL-1 .Conclusion The HPLC-MS/MS method was accurate, sensitive, specific, and could be used in the determination of sunitinib in plasma and the clinical study of its pharmacokinetics.

Objective To develop a simple and highly sensitive liquid chromatography-tandem quadrupole mass spectrometry method for deter-mination of estradiol in human plasma.Method Estradiol was extrac-ted using liquid-liquid extraction method from human plasma, with est-rodial-d3 as an internal standard.After extracted, samples were deri-vatizated by dansyl chloride in alkaline conditions.The Atlantis T3 (2.1 mm ×100 mm, 5.0 μm) column was used for separation.The gradient mobile phase was 0.1% formic acid and acetonitrile with a flow rate of 0.5 mL? min-1 , the temperature was set at 30 ℃, and the injection volume was 10 μL.The detection was performed with multiple reactions monitoring using positive electrospray ionization with API5500 as the detec-tor.Results The linear ranges of estradiol was 1 -100 pg? mL-1 , lower limit of quantification was 1 pg? mL-1 , the regression equation was y=5.47 ×10 -2 x+4.67 ×10 -2 .The inter-and intra-day RSD were less than 15%and the absolute recovers were above 97%.Estrodiol was proved to be stable under study station.Conclusion This method is specific, sensitive, accurate, and suitable for the pharmacokinetic study of estradiol in human plasma.

Objective Subjects failed to filter the reasons were summarized through the analysis of phase Ⅰ clinical trial process.Methods The reasons for the screening failure were summarized and the possible influencing factors were discussed by analyzing the screening process in a clinical trial of cardiovascular drugs that involved 106 cases of healthy adults.Results Compliance,physical examination,laboratory tests and special examinations may be related to the screening failure,among which laboratory test failure was the leading cause (39.76％) followed by Holter electrocardiogram (28.89％),while echocardiography and compliance factors respectively accounted for 10.0％ and 10.38％.Conclusion The screening success rate can be improved by developing a suitable range of laboratory normalization and depth of knowledge.

Objective To establish a HPLC-MS/MS method for simul-taneous determination of diazapam and its three metabolites in human liv-er microsomal.Methods Kromasil C18 column was used at 40 ℃, and the mobile phase was a mixed system consisting of acetonitrile -2 mmol? L-1 mammonium acetate ( 70 ∶30 ) and a flow rate of 0.3 mL? min -1 .The detection of the samples was made using positive ion scan and multiple reaction monitoring mode.The specificity, linearity and limit of quantification, precision and recovery, matrix effect and sta-bility were assessed.Results The sample showed good linearity from 20-1000 ng? mL-1 ( r>0.99 ) and the lower limit of quantitation was 20 ng? mL-1 .The liner calibration curve of nordiazepam, temazepam and oxazepam obtained concentration range of 2 -100 ng? mL-1 (r>0.99) and the low limit of quantitation was 2 ng? mL-1 .The average recovery rates of diazepam, nordiazepam, temazepam and oxaze-pam were 55.47% to 68.76%, 64.84% to 79.11%, 68.32% to 78.27%and 72.66%to 83.82%.The intra-day and inter-day relative standard deviations were both less than 15%.Conclusion The method is simple, rapid and accurate, suitable for measuring the concentration of diazapam and its three metabolites in human liver microsomal.