1.Binding of EGF1 domain peptide in coagulation factor VII with tissue factor and its implications for the triggering of coagulation.
Heng, MEI ; Yu, HU ; Huafang, WANG ; Wei, SHI ; Jun, DENG ; Tao, GUO
Journal of Huazhong University of Science and Technology (Medical Sciences) 2010;30(1):42-7
The binding function of EGF1 domain peptide with tissue factor (TF) and its ability of triggering coagulation were explored. The TF expression model in vitro was established by lipopolysaccharide induction. The affinity of EGFP-EGF1 and TF expressing cells was analyzed by fluorescence microscopy and flow cytometry (FCM). The affinity of EGFP-EGF1 and rat soluble TF was quantitated by surface plasmon resonance (SPR). The ability of EGFP-EGF1 in triggering coagulation was tested by prothrombin time assay. The FCM results showed recombinant factor VII (rFVII) could definitely depress the integration of EGFP-EGF1 with recombinant TF (rTF) (68.65%+/-3.86% vs 57.98%+/-4.71%, P<0.01). The SPR results indicated the association constant ka of EGFP-EGF1 proteins was higher than rFVII (8.29+/-1.39 vs 3.75+/-0.32, P<0.01). However, the EGFP-EGF1 protein lost the activity of triggering coagulation as compared with blood plasma of normal SD rats (56.8+/-3.2 s vs 17.8+/-3.4 s, P<0.01). It was concluded that the rat EGF1 peptide could specifically bind to TF without the ability of triggering coagulation. EGF1 peptide may be a good target head for delivering drugs to TF in anticoagulation therapy.
2.An analysis about early and long-term curative effect of 56 cases of completion pneumonectomy
Jicheng TANTAI ; Xufeng PAN ; Shijie FU ; Jianxin SHI ; Jun YANG ; Heng ZHAO
Chinese Journal of Thoracic and Cardiovascular Surgery 2017;33(2):91-94
Objective This study was to analyze the early and long-term effect of completion pneumonectomy.Methods Retrospective analysis was made on the patients who underwent completion pneummonectomy in Shanghai Chest Hospital.Results There were totally 56 cases patients underwent completion pneumonectomy during January 2003 to July 2013.Among them,45 patients received CCP,and other 11 patients received RCP.CCP refers to the complete removal of lung tissue remaining after an initial ipsilateral partial pulmonary resection.RCP refers to the complete removal of residual lung due to the severe complications after pneumonectomy.The mortality and morbidity rate of CCP were 4.4% and 33.3% respectively.In the case of CCP,the incidence of benign lesions is significantly higher than the incidence of malignant tumor(80.0% vs 27.5%,P =0.04).The mortality and morbidity rate of RCP were 27.3% and 90.9% respectively.In the case of RCP,higher postoperative mortality often occurs in aged patients (P =0.046) and patients with preoperatie mechanical ventilation (P =0.03).Overall five-year survival rate for patients with benign lesions was 80%,and for malignant lung cancer patients,the number was 30%.Survival time differs according to the TNM staging(a median of 60.0 months,35.0 months,10.0 months,stage Ⅰ,stage Ⅱ,stage Ⅲ,P <0.01),and survival rate was higher when the time interval(between the initial pulmonary resection and the completion pneumonectomy) > 2 years(a median of 60.0 months,18.0 months,P < 0.01).Conclusion Completion pneumonectomy is a high-risk surgery,especially RCP.Advanced age and preoperative mechanical ventilation are associated with higher postoperative mortality rate for RCP.As for CCP,higher postoperative risk exists in patients with benign lesions,but the survival rate is also higher.In patients with malignant lung tumor,survival rate is higher when the time interval (between the initial pulmonary resection and the completion pneumonectomy) >2 year.
3.Acute kidney injury related to crush syndrome during Wenchuan earthquake
Bo SHI ; Tianfu YANG ; Jun WANG ; Shitian TANG ; Bin KANG ; Heng YANG ; Lin ZHANG ; Xiaohui GAN
Chinese Journal of Emergency Medicine 2009;18(6):640-644
Objective To identify risk factors associated with the severity of acute kidney injury (AKI) in-duced by crush syndrome and whether the patient required hemodialysis (HD). Method A retrospective study was designed. Within 19 days after the Chinese Wenchuan earthquake (May 12, 2008), 63 victims (33 men, 30women) of 2139 cases were hospitalized at Mianyang Central Hospital (Siehuau, China) because of crush syn-drome caused by crush injuries. The patients with renal dysfimcfion before the earthquake were excluded. Totally 63 patients with AKI associated with crush syndrome were included in this study and were divided into two groups: group 1, 25 patients, requiring HD (when urine volume <250 mL/d;serum potassium> 6 mmol/L) ; and group 2, 38 patients, without HD. The following data were collected retrospectively for all patients: (1) epidemiological parameters: age, gender, race, time under the rubble, liquid treatmem before being rescued; (2) clinical param-eters: blood pressure, body area crushed, amputation, fasciotomy, blood transfusion, quantity of fluid infusion, urine output in the first 24 hours; (3) initial laboratory data: complete blood count, urine analysis, serum chem-istry, arterial blood gas analysis. Comparisons between the two groups were made using SPSS 10.0. The quantita-tive data and categorical data were analyzed using t tests and χ2 tests, respectively. P -values < 0.05 were consid-ered to indicate significant differences. The significant variables were entered into logistic regression models to de-termine the risk factors for the severity of AK1 in patients with crush syndrome and whether the patient required HD. Results Four significant risk factors with P -values < 0.05 were identified: fasciotomy, cystatin C (Cys C)level, myoglobin (MB) level and lactic acidosis. The odds ratios (95% confidence intervals) were 8.641 (3.027~76.479), 6.956 (3.027~76.479), 5.379 (3.027~76.479) and 4.833 (2.569~32.764), respectively. Conclusions In addition to urine output and potassium levels, we found that four risk factors, namely faseiotomy, Cys C and MB levels, and lactic acidosis, were significanfly associated with the severity of AKI and whether the patient required hemodialysis.
4.Secretory carcinoma of breast in male: report of a case.
Yan XU ; Qun-Li SHI ; Xiao-Jun ZHOU ; Heng-Hui MA ; Hang-Bo ZHOU
Chinese Journal of Pathology 2009;38(10):707-708
Adenocarcinoma, Mucinous
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metabolism
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pathology
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Adult
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Breast Neoplasms
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metabolism
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pathology
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surgery
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Breast Neoplasms, Male
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metabolism
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pathology
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surgery
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Cadherins
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metabolism
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Carcinoma
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metabolism
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pathology
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surgery
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Carcinoma, Signet Ring Cell
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metabolism
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pathology
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Diagnosis, Differential
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Follow-Up Studies
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Humans
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Keratin-5
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metabolism
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Lymph Node Excision
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Male
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Mastectomy
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methods
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Mucin-1
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metabolism
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S100 Proteins
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metabolism
5.Detection of?-catenin and c-myc expression in gastric adenocarcinoma by tissue microarray and its clinical significance
Wei-Chang CHEN ; Dong-Tao SHI ; Heng-Jun GAO ; Baofeng ZHANG ; Ying HU ;
Chinese Journal of Digestion 2001;0(12):-
Objective To study the expression and clinical significance of?-catenin and c-myc in gastric adenocarcinoma and corresponding para cancer gastric mueosa.Methods Two hundreds and ninety seven samples were collected from 102 cases of gastric adenocarcinoma,including 102 cancerous tissues and 195 para-cancerous tissues.Each sample was made into 282,156 and 156 dots tissue microarrays.Expressions of?-catenin and c-myc proteins were detected by immunohistochemical stain- ing.Results The expressions of?-catenin and c-mye were increased gradually in the process of gastric carcinogenesis.The rates of?-catenin and c myc expression were higher in carcinoma than that in intesti- nal metaplasia( P<0.05,P<0.001).The aberrant expression of?-catenin was closely related to the depth of invasion(P<0.05),and the expression of c-myc was related to histological grade( P<0.05). The aberrant expression of?-catenin was significantly correlated with the expression of c-myc in gastric adenocarcinoma(P<0.05).Conclusions The aberrant expression of?-catenin may activate the expres sion of target gene c-myc,which plays a pivotal role in the carcinogenesis and progression of gastric adenocarcinoma.
6.Primary testicular yolk sac tumor: clinicopathological study of 8 cases.
Fei-Fei LIU ; Jian-Jun WANG ; Qin SHEN ; Bo YU ; Zhen-Feng LU ; Heng-Hui MA ; Qun-Li SHI
National Journal of Andrology 2014;20(5):435-438
OBJECTIVETo investigate the clinicopathological characteristics, diagnosis and treatment of primary testicular yolk sac tumor (YST).
METHODSWe studied 8 cases of primary testicular YST by microscopy and immunohistochemistry.
RESULTSThe 8 cases of primary testicular YST, including 2 consultation cases, were confirmed from 1998 to 2013, accounting for 10.7% (8/75) of all the testicular germ cell tumors diagnosed in our hospital. The patients ranged in age from 7 to 43 years, 23.9 years on average. The main clinical manifestation of the patients was painless unilateral testis swelling. Microscopically, reticular tissues, schiller-duvaI (S-D) bodies, and eosin-stain transparent bodies were seen in the tumors. One of the cases was confirmed to be simple YST, while the other 7 mixed YST. AFP was a characteristic immunophenotype marker of the tumors.
CONCLUSIONPrimary testicular YST is a rare malignancyr with poor prognosis. Its diagnosis depends on preoperative AFP test and postoperative pathology. Comprehensive treatment, including orchiectomy, chemotherapy, and radiotherapy, can prolong the survival of the patients.
Adolescent ; Adult ; Child ; Endodermal Sinus Tumor ; metabolism ; pathology ; therapy ; Humans ; Immunohistochemistry ; Male ; Neoplasms, Germ Cell and Embryonal ; metabolism ; pathology ; therapy ; Orchiectomy ; Rare Diseases ; metabolism ; pathology ; therapy ; Testicular Neoplasms ; metabolism ; pathology ; therapy ; Young Adult
7.Application and value of fluorescence quantitative PCR in detecting Mycobacterium tuberculosis in paraffin embedded specimens.
Chun-ying LUO ; Jian-dong WANG ; Xuan WANG ; Heng-hui MA ; Shan-shan SHI ; Bo YU ; Xiao-jun ZHOU
Chinese Journal of Pathology 2012;41(8):562-563
Adolescent
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Adult
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Aged
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Aged, 80 and over
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Female
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Fluorescence
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Follow-Up Studies
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Humans
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Male
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Middle Aged
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Mycobacterium tuberculosis
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isolation & purification
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Paraffin Embedding
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Polymerase Chain Reaction
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methods
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Retrospective Studies
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Tuberculosis, Pulmonary
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diagnosis
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microbiology
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Young Adult
8.Association of the common genetic variant upstream of INSIG2 gene with obesity related phenotypes in Chinese children and adolescents.
Hai-Jun WANG ; Heng ZHANG ; Shi-Wei ZHANG ; Yong-Ping PAN ; Jun MA
Biomedical and Environmental Sciences 2008;21(6):528-536
OBJECTIVETo study the association between the rs7566605 variant of INSIG2 and obesity-related phenotypes in Chinese children and adolescents.
METHODSThe study sample consisted of two independent cohorts of Chinese children and adolescents. Anthropometric indices, lipids, blood pressure, fasting glucose, insulin and percentage of fat mass were determined. PCR with restriction fragment length polymorphism analysis was performed for genotyping the rs7566605 variant.
RESULTSIn each of the two independent cohorts, no significant association was observed between rs7566605 and obesity under additive, dominant or recessive model. We also did not detect any difference in the genotype frequency between all the obese children and controls. Furthermore, we did not find evidence of an association between body composition indices and metabolic phenotypes in all children. However, the triglyceride level of CC homozygotes was significantly higher than that of GG+GC genotypes in obese children (P=0.022). Additionally, we observed a non-significant trend of severe obesity in a post-hoc test.
CONCLUSIONINSIG2 rs7566605 variant is not associated Chinese childhood obesity in two independent cohorts. Further study is needed to verify the effect of rs7566605 on triglyceride in obese children.
Adipose Tissue ; Adolescent ; Asian Continental Ancestry Group ; ethnology ; genetics ; Body Mass Index ; Child ; China ; Cohort Studies ; Female ; Genetic Variation ; genetics ; Humans ; Intracellular Signaling Peptides and Proteins ; genetics ; Male ; Membrane Proteins ; genetics ; Obesity ; ethnology ; genetics ; Phenotype ; Risk Factors ; Young Adult
9.Expression of nuclear factor kappaB and tumor necrosis factor α in lung tissue of paraquat poisoned rats.
Jun-li HU ; Han-wen SHI ; Ying-ping TIAN ; Xia WANG ; Heng-bo GAO ; Fei TONG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2011;29(1):44-48
OBJECTIVETo investigate the expression of nuclear factor kappa B (NF-kgr;B) and tumor necrosis factor α (TNF-α) in lung tissue of acute paraquat poisoned rats.
METHODS68 male Wistar rats were randomly divided into 2 groups: the control group (n = 8), the intoxication group (n = 60). On the 1st, the 3rd, the 7th, the 14th and the 28th day after intoxication, the expression of NF-κB p65 and TNF-α in lung tissue were detected by LSAB immunohistochemistry (IH) staining. Meanwhile, the level of malondialdehyde (MDA) in plasma, and lung homogenate, the content of malondialdehyde (HPY) in lung homogenate were detected.
RESULTSThe levels of MDA in plasma on the 1st, the 3rd, the 7th day and in lung homogenate on the 1st, the 3rd day of the intoxication group [in plasma: (10.15 ± 3.15), (6.97 ± 1.65) and (5.44 ± 0.66) nmol/ml; in lung homogenate: (10.20 ± 2.43), (10.71 ± 171) nmol/ml] were significantly higher than that of the control group [in plasma: (3.84 ± 1.04) nmol/ml, in lung homogenate: (7.66 ± 0.66) nmol/ml]. The content of HPY in lung homogenate on the 14th and the 28th day after intoxication [(19.98 ± 2.86), (26.06 ± 4.06) µg/0.1 g lung homogenate] were higher than that of the control group [(8.80 ± 1.26) µg/0.1 g lung homogenate] significantly. The expression of NF-κB p65 and TNF-α in lung tissue were both significantly increased on the first day and the 3rd day of the intoxication group compared with the control group and weakened obviously after the 7th day.
CONCLUSIONAcute paraquat poisoning can induce increased expression of both NF-κB p65 and TNF-α in lung tissue; the enhanced activity of NF-κB may take part in the process of pulmonary injury in PQ poisoning.
Animals ; Lung ; metabolism ; pathology ; Male ; Paraquat ; poisoning ; Rats ; Rats, Wistar ; Transcription Factor RelA ; metabolism ; Tumor Necrosis Factor-alpha ; metabolism
10.Emergency operation for the treatment of intertrochanteric fractures in elderly patients.
Bo SHI ; Jun WANG ; Heng YANG ; Ding-Wei ZHANG ; Gang LIU ; Tao WANG ; Nan YE
China Journal of Orthopaedics and Traumatology 2013;26(5):408-411
OBJECTIVETo evaluate the emergency therapeutic effects of intertrochanteric fractures in elderly patients.
METHODSA retrospective study was designed. From March 2006 to March 2012, 254 patients with intertrochanteric fractures treated in Mianyang Central Hospital were reviewed. The patients with old fractures, pathological fractures, multiple trauma and severe medical comorbidities of admission were excluded. So 127 patients with intertrochanteric fractures over 70 years old were included in the study, including 38 males and 89 females, and the patients were divided into 2 groups: 56 patients underwent emergency operation within 24 h after injury (emergency operation group), 71 patients underwent an operation more than 24 h after injury (not emergency operation group). The following data were collected retrospectively for all patients: (1) in-hospitalcomplications, including electrolyte disturbance, hidden blood loss, delirium, deep vein thrombosis, upper gastrointestinal hemorrhage, pulmonary infection, urinary tract infection, cardiovascular and cerebrovascular disease, bedsore. (2) prognosis, ineluding in-hospital mortality, mortality in the 1st year, length of hospital stay, fracture healing times, hip score. Comparisons between the two groups were made using SPSS13.0. The quantitative data and categorical data were analyzed by t test and chi2 test, respectively.
RESULTSThe main medical complications in emergency operation group were hidden blood loss, delirium, pulmonary infection, electrolyte disturbances, urinary tract infections, and in not emergency operation group followed respectively by hidden blood loss, lung infection, electrolyte disturbances, delirium, and pulmonary infection. In-hospital mortality was zero, and 1 in the 1st year in emergency operation group; while in not emergency operation group were 4 and 7 respectively. An average length of hospital stay with (9 +/- 3) d in emergency operation group was lower than (17 +/- 8) d in not emergency operation group. Fracture healing time was (12 +/- 2) weeks in both groups. The significant variables with P < 0.05 between the two groups were electrolyte disorders, delirium, deep venous thrombosis, pulmonary infection, urinary tract infection, bedsore, in-hospital mortality, mortality in the 1st year, and length of hospital stay. The differences of hidden blood loss, upper gastrointestinal hemorrhage, cardiovascular and cerebrovascular disease, fracture healing times, hip score between the two groups had no significant differences (P > 0.05).
CONCLUSIONThe emergency surgery (within 24 h after injury) reduces length of hospital stay, and may also reduce complications and mortality.
Aged ; Aged, 80 and over ; Case-Control Studies ; Female ; Hip Fractures ; mortality ; surgery ; Humans ; Male ; Postoperative Complications ; Retrospective Studies ; Treatment Outcome