BACKGROUND: We evaluated the performance and analysis time of HLC-723 G7 (Tosoh corp. Tokyo, Japan) hemoglobin (Hb) A1c autoanalyzer. It utilizes cation exchange high performance liquid chromatography (HPLC) method and has a reduced analysis time compared with that of an earlier model HLC-723GHb V A1c 2.2(TM) (HLC-723GHb V, Tosoh corp. Tokyo, Japan). METHODS: We evaluated linearity, precision and comparison with HLC-723GHb V following NCCLS guidelines and counted the number of tests per hour to estimate analysis time. RESULTS: Linearity through the range from 5.8% to 13.9% was good (r2=0.9930, relative nonlinearity <2.5%). The within-run coefficients of variation (CVs) for groups of low, middle, and high level were 1.09%, 0.76%, and 0.68% and total CVs for each group were 1.60%, 0.91%, and 1.00%, respectively. Correlation equation between HLC-723 G7 and HLC-723GHb V was HLC-723 G7=1.0308 (HLC-723GHb V)-0.2896 %Hb A1c (r=0.9992, P<0.0001). Analysis time of HLC-723 G7 was 1.2 minutes per test compared with 2.1 minutes of HLC-723GHb V. CONCLUSIONS: HLC-723 G7 showed the acceptable performance and shortening analysis time therefore, it was suitable for reducing turn around time of Hb A1c assay.
Chromatography, Liquid ; Hemoglobin A, Glycosylated

No abstract available.
Blood Glucose* ; Hemoglobin A, Glycosylated*

No abstract available.
Hemoglobin A, Glycosylated* ; Humans

No abstract available.
Hemoglobin A, Glycosylated* ; Humans

The relationship between the relative amounts of HbH and the proportions of RBC with inclusion bodies (IBs) as shown that almost straight, getting a correlation coefficient of 0.86, the regression line cuts the x- axis of RBC with lbs at 19%, indicating that demonstration of RBC with IBs is more sensitive in detecting HbH than electrophoresis. Strickingly the mean level of both HbH and percentage of RBC with lbs were significantly higher in a- than 1/HbCS than in a- than 1/ a -than2. These results led us to conclude that examination of the intraerythrocytic inclusion bodies is able to be used as a “probe” technique in unequiped laboratory for detecting HbH disease.
Erythrocytes ; Hemoglobin H

Optimal glycaemic control is crucial in patients with diabetes mellitus (DM) to avoid episodes of hypoglycaemia and both micro- and macrovascular complications. Diabetic control relies mainly on the evaluation of haemoglobin A1c (HbA1c), which is unfortunately unreliable in patients with haemoglobinopathies. This case report describes a patient with type 2 DM and haemoglobin J (HbJ) trait, which resulted in erratic HbA1c values throughout her follow-up. Other approaches, such as self-blood glucose monitoring, are needed to evaluate glycaemic control instead of relying on HbA1c alone to guide the management DM in these patients.
Glycated Hemoglobin ; Hemoglobinopathies

No abstract available.
Adult ; Fasting ; Glucose ; Hemoglobin A, Glycosylated ; Hemoglobin, Sickle ; Humans ; Plasma

No abstract available.
Adult ; Fasting ; Glucose ; Hemoglobin A, Glycosylated ; Hemoglobin, Sickle ; Humans ; Plasma

No abstract available.
Cystatin C* ; Hemoglobin A, Glycosylated* ; Humans

No abstract available.
Hemoglobin A* ; Humans ; Mothers* ; Postpartum Period*