1.Advancements in the study of the classification and immune function of shrimp hemocytes.
Chinese Journal of Biotechnology 2021;37(1):53-66
Hemocytes play an important role in the immune defense system of animals, especially for invertebrates that have no adaptive immune system. In those animals, hemocytes not only participate in the cellular immunity including phagocytosis, encapsulation, and nodules formation, but also the humoral immunity via storage and release of immune factors. Identification of the components of hemocytes is the basis for understanding the immune mechanism and the function of hemocytes. Despite various researches have been done on distinguishing the composition and function of shrimp hemocytes, no standard is used uniformly until now. So, we analyze and summarize the results on shrimp hemocytes research and offer a three subgroups category in this review. We also introduce the morphological characters and immune function of three subgroups in detail. We hope this work will be beneficial for understanding the function and molecular mechanism of hemocytes in invertebrate, bringing ideas for new separation technology development.
Animals
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Hemocytes
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Phagocytosis
2.Some opinions of level of hemocyte in peripheral blood of women in Th¸i B×nh countryside
Journal of Practical Medicine 2002;435(11):30-31
A study on hemocyte in peripheral blood of women ages of 18 and above in some suburb communes in Th¸i B×nh province has shown that level of average Hb is about 120 - 130 g/l, highest level of it is in ages of 18 -30 (141, 44 g/l) and lowest in ages of 70 and above (123,66g/l). The rate of subjects with Hb level less than average normal threshold (120% g/l) is 24,76%. This rate is 20,16% in ages of 41 -50 and highest rate is 48,48% in ages of 70 and above mild anemia (most common); moderate anemia: uncommon; and severe anemia: 0.
Hemocytes
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Hemoglobins
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Anemia Peripheral Blood Stem Cell Transplantation
3.Non-specific Defensive Factors of the Pacific Oyster Crassostrea gigas against Infection with Marteilioides chungmuensis: A Flow-Cytometric Study.
Hee Jung CHOI ; Jee Youn HWANG ; Dong Lim CHOI ; Min Do HUH ; Young Baek HUR ; Nam Sil LEE ; Jung Soo SEO ; Mun Gyeong KWON ; Hye Sung CHOI ; Myoung Ae PARK
The Korean Journal of Parasitology 2011;49(3):229-234
In order to assess changes in the activity of immunecompetency present in Crassostrea gigas infected with Marteilioides chungmuensis (Protozoa), the total hemocyte counts (THC), hemocyte populations, hemocyte viability, and phagocytosis rate were measured in oysters using flow cytometry. THC were increased significantly in oysters infected with M. chungmuensis relative to the healthy appearing oysters (HAO) (P<0.05). Among the total hemocyte composition, granulocyte levels were significantly increased in infected oysters as compared with HAO (P<0.05). In addition, the hyalinocyte was reduced significantly (P<0.05). The hemocyte viability did not differ between infected oysters and HAO. However, the phagocytosis rate was significantly higher in infected oysters relative to HAO (P<0.05). The measurement of alterations in the activity of immunecompetency in oysters, which was conducted via flow cytometry in this study, might be a useful biomarker of the defense system for evaluating the effects of ovarian parasites of C. gigas.
Animals
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Cell Count
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Cell Survival
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Cercozoa/*immunology/*pathogenicity
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Crassostrea/*immunology
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Flow Cytometry
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Granulocytes/immunology
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Hemocytes/immunology
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Phagocytosis
4.Pancytopenia Associated with Hypopituitarism in an Acromegaly Patient: A Case Report and a Review of the Literature.
Jung Hee KOH ; Yong Jae LEE ; Ji Hyun KANG ; Bo Kwang CHOI ; Yun Kyung JEON ; Sang Soo KIM ; Bo Hyun KIM ; In Joo KIM
Endocrinology and Metabolism 2012;27(4):308-313
We present the case of a patient with acromegaly who had pancytopenia with hypopituitarism secondary to the excision of a pituitary macroadenoma and radiation therapy. A 28-year-old man presented with pancytopenia and serum electrolyte abnormalities. He was diagnosed with acromegaly and underwent surgery and gamma-knife radiotherapy for a pituitary macroadenoma at the age of 22 years. A recent brain magnetic resonance imaging showed an empty sella, and the basal hormonal profile demonstrated deficiencies of pituitary hormones except thyrotropin. As presenting pancytopenia, his bone marrow biopsy showed hypocellular marrow. The total number of hemocytes increased after hydrocortisone replacement. Hypopituitarism was a possible cause of pancytopenia, and glucocorticoids had crucial effects on converting pancytopenia to normal in this case.
Acromegaly
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Adult
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Biopsy
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Bone Marrow
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Brain
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Glucocorticoids
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Hemocytes
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Humans
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Hydrocortisone
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Hypopituitarism
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Magnetic Resonance Imaging
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Pancytopenia
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Pituitary Hormones
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Thyrotropin
5.Identification and characterization of a novel hemocyte specific gene Bm04862 in silkworm, Bombyx mori.
Shuang YU ; Jingjing SU ; Man XU ; Kui ZHANG ; Hongjuan CUI
Chinese Journal of Biotechnology 2016;32(2):241-249
Bm04862 is a novel hemocyte-specific gene, which has been identified and cloned through the microarray data in silkworm, Bombyx mori. Initially, we successfully obtained the full-length cDNA sequence of Bm04862 via the rapid amplification of cDNA ends (RACE). The sequence consists of an open reading frame (ORF) with 819 bp, which could encode 273 amino acid residues. The bioinformatics analysis predicted that Bm04862 was a transmembrane protein. Furthermore, qRT-PCR analysis revealed that the expression of Bm04862 was specifically detected in hemocyte and reached a peak at L4M and PP2 stage. Bm04862 was overexpressed in Sf9 insect cells, and the cellular localization indicated that Bm04862 was specifically located in cytoplasm and nuclei membrane. Interestingly, the expression of Bm04862 increas'd dramatically after challenged with Escherichia coli for 24 hours, which predicted its potential role in the innate immune system. Overall, this study could provide the fundamental knowledge for further research.
Animals
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Bombyx
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genetics
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Cloning, Molecular
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Computational Biology
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DNA, Complementary
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Hemocytes
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metabolism
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Insect Proteins
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genetics
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metabolism
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Membrane Proteins
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genetics
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metabolism
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