In July 2002, a destructive stem rot of tawny daylily(Hemerocallis fulva) was occurred sporadically in exhibition farm of Gyeongsangnam-do Agricultural Research and Extension Services located in Hamyang-gun, Korea. The fungus also caused collar and crown rot, and systemic wilt or blight of whole plant. White mycelium spread over stems and petioles of infected plants and sclerotia were formed on the old lesions and near the soil surface. The optimum temperature for mycelial growth and scierotial formations was 30degrees C on PDA. The mycelial width ranged 4.2~10.4microm and the color was white, usually many narrow mycelial strand grew in the aerial mycelium and formed clamp connection. The shape of sclerotia was spherical and 1.0~3.2 mm in diameter. The fungus was isolated repeatedly from the infected tissues and confirmed its pathogenicity to Hemerocallis fulva and identified as Sclerotium rolfsii. This is the first report on the stem rot of H. fulva caused by S. rolfsii in Korea.
Crowns ; Fungi ; Gyeongsangnam-do ; Hemerocallis* ; Korea* ; Mycelium ; Plants ; Soil ; Virulence

OBJECTIVETo study the chemical constituents of the root of Hemerocallis fulva.

METHODCompounds were isolated by repeated silica gel, Sephadex LH-20, MCI gel, etc. column chromatography and their structures were determined by spectral analyses and physicochemical properties.

RESULTFrom the n-butanol fraction of the EtOH extract of the roots of H. fulva, 9 glycosides were isolated and identified as sweroside (1), laganin (2), picraquassioside C (3), puerarin (4), 3'-methoxypuerarin (5), 7-hydro xylnaphthalide-O-beta-D-glucopyranoside (6), orcinol-3-O-beta-glucopyranoside (7), HN saponin F (8), hederagenin-3-0-beta D-glucopyranosyl-(1-3)-alpha-L-arabinopyranoside-28-O-beta-D-glucopyranosyl ester (9).

CONCLUSIONCompounds 1-9 were isolated from the genus Hemerocallis for the first time.

Glucosides ; chemistry ; isolation & purification ; Hemerocallis ; chemistry ; Iridoid Glucosides ; Iridoids ; chemistry ; isolation & purification ; Isoflavones ; chemistry ; isolation & purification ; Magnetic Resonance Spectroscopy ; Plant Roots ; chemistry ; Saponins ; chemistry ; isolation & purification

This study is designed to explore the possible effects of Hemerocallis citrina baroni flavonids (HCBF) on liver fibrosis induced by CCl4 in rats. The liver fibrosis model was induced by CCl4, and HCBF were administered by gastric perfusion at 25 and 50 mg x kg(-1) qd for 50 days, while the contents of alanine transaminase (ALT), aspartate aminotransferase (AST), gamma glutamyl transpeptidase (GGT), alkaline phosphatase (ALP), superoxide dismutase (SOD), maleic dialdehyde (MDA) and transforming growth factor-β1 (TGF-β1) were measured and the contents of PINP were measured in liver tissue, and the expression of TGF-β1 were observed by immunohistochemisty and Western blot. The pathological changes of liver tissue were examined by HE. The results showed that HCBF (25, 50 mg x kg(-1)) improved the liver function significantly through reducing the level of ALT, AST, GGT and ALP (P < 0.05 or P < 0.01), and increasing the content of SOD (P < 0.01), while reducing the content of MDA (P < 0.05 or P < 0.01), the expression of TGF-β1 (P < 0.05) and the content of PINP (P < 0.05). The results suggest that HCBF (25, 50 mg x kg(-1)) may inhibit the liver injury induced by CCl4 by decreasing the oxidative stress.
Alanine Transaminase ; metabolism ; Alkaline Phosphatase ; metabolism ; Animals ; Aspartate Aminotransferases ; metabolism ; Carbon Tetrachloride ; Drugs, Chinese Herbal ; pharmacology ; Flavonoids ; pharmacology ; Hemerocallis ; chemistry ; Liver Cirrhosis ; chemically induced ; drug therapy ; Malondialdehyde ; metabolism ; Oxidative Stress ; drug effects ; Plant Extracts ; pharmacology ; Rats ; Superoxide Dismutase ; metabolism ; Transforming Growth Factor beta1 ; metabolism ; gamma-Glutamyltransferase ; metabolism