Telepathology systems will be common systems in hospitals. The two systems were designed and implemented in web environments for test. One was implemented with the Common Object Request Broker Architecture (CORBA) technique. The other system was implemented in the form of ActiveX. The histopathological materials were stained by Hematoxylin and Eosin. By the Donpisha CCD camera attached to an Olympus BX-51 optical microscope 180 color images come to be acquired. For evaluation of the systems, transmission times and telediagnosis concordance rates were measured. Image processing ability was tested using two telepathology systems. For the local area test, system I using CORBA had measured image transmission times of 0.1 s, 0.2 s, and 0.4 s at the file sizes of 100 K byte, 900 K byte and 3.6 M byte respectively. Transmission times for system II using Component Object Model (COM) were slightly slower, ranging from 0.02 s to 0.05 s. In the long distance area test, system II transmission times were 0.5 s, 0.8 s, and 2.0 s. The overall concordance rate of telediagnosis for the 180 images was 78.3%. In this study, we compared our systems about image transmission, and processing for the further development of system configurations.
Eosine Yellowish-(YS) ; Hematoxylin ; Internet* ; Telepathology*

OBJECTIVE: To explore the treatment conditions of acid decalcified specimens and improve the poor quality of sections and unclear structure of hematoxylin-eosin (HE) staining caused by the change in pH in tooth and hard tissue after acid decalcification. METHODS: A total of 20 cases of oral pathological specimens that contain hard tissues were decalcified and treated with routine treatment, concentrated ammonia water immersion treatment, and saturated lithium carbonate solution immersion treatment. The quality and HE staining effects of hard tissue sections treated with different methods were compared. RESULTS: Compared with routine treatment, lithium carbonate saturated solution treatment showed complete sections. Hematoxylin is strongly stained, the nucleus is clear, and the cytoplasm is bright. CONCLUSIONS Soaking acid decalcified specimens in lithium carbonate saturated solution before embedding in dehydration can neutralize the acidic environment of the tissue. The quality of sections and HE staining effect are improved and are suitable for the pretreatment of acid decalcified tissue samples of oral pathology.
Eosine Yellowish-(YS) ; Hematoxylin ; Staining and Labeling ; Tooth

PURPOSE: We report our experience with a case of a nodular lesion of the lower palpebral conjunctiva with histologic features consistent with mucoepidermoid carcinoma. METHODS: A 72 year-old man presented with a left lower palpebral conjunctiva lesion. 3 x 3 mm sized flat pedunculated yellowish red mass was observed. Incisional biopsy proved the mass a mucoepidermoid carcinoma and wide local resection was conducted. RESULTS: Hematoxylin and eosin-stained sections showed lobules of tumor cells, consisting of predominantly epidermoid cells and scattered islands of mucin-secreting cells. Mucicarmine-stained sections demonstrated strong positivity, consistent with the diagnosis of mucoepidermoid carcinoma. CONCLUSIONS: In any case presenting palpebral conjunctival lesion, mucoepidermoid carcinoma should be differentiated, and it is necessary to confirm the diagnosis through biopsy.
Aged ; Biopsy ; Carcinoma, Mucoepidermoid* ; Conjunctiva* ; Diagnosis ; Hematoxylin ; Humans ; Islands

For comparison of clinical classification of leprosy to histopathologic classification, a detailed histopathologic study, using hematoxylin and eosin stain and Ziehl-Neelsen stain, was done on 72 fresh uncomplicated cases of leprosy. The clinical classification was done using the criteria of Ridley and Jopling (1966), and the microscopic features were classified according to Ridley's(1974) definition. Clinical classification revealed that 8 of total 72 patients had tuberculoid(TT), 9 had borderline tuberculoid (BT), 5 had borderline(BB), 10 had borderline lepromatous.(BL), and 31 had lepromatous leprosy(LL). Nine patients were claasified as indeterminate(I) group. Histopathologic classification showed that 3 cases presented tuberculoid(TT), 10 presented borderline tuberculoid(BT), 4 presented borderline(BB), 9 presented borderline lepromatous(BL), 20 presented subpolar leprornatous(LLs), and 10 presented polar lepromatous(LLp) histopathologic characteristics, Sixteen cases were classified as indeterminate(I) leprosy by histopathologic findings. On comparison of clinical classification to histopathologic classification, the two were in consonance with each other in 50 cases(69.4%) and the disparity between them was noticed in 22 cases(30.6%). Among the 22 cases which showed disparity, there was a shift of one step either towards the tuberculoid or lepromatous end of the spectrum in 15 cases, and ihe remaining 7 cases were classified as indeterminate group beaause of nonspecific histopathologic changes.
Classification* ; Eosine Yellowish-(YS) ; Hematoxylin ; Humans ; Leprosy* ; Leprosy, Paucibacillary

Eosine Yellowish-(YS) ; Frozen Sections ; Hematoxylin ; Humans ; Staining and Labeling

We tested a set of conditions for obtaining optimal tissue quality in preparation for histology in samples of mouse brain. C57BL/6J mice were sacrificed and perfused with 4% paraformaldehyde, after which the brains were removed and dehydrated in 30% sucrose solution. The brains were then divided into four groups according to freezing temperature and usage of optimal cutting temperature (OCT) compound. Next, we stained the sectioned brain tissues with Harris hematoxylin and eosin Y and immunohistochemistry was performed for doublecortin. The best quality tissue was obtained at -25℃ and by not embedding with the OCT compound. When frozen at -25℃, the embedded tissue was significantly damaged by crystals, while at -80℃ there were no meaningful differences between qualities of embedded- and non-embedded tissues. Overall, we identified a set of conditions to obtain quality frozen brain sections. Our developed protocol will help resolve matters associated with damage caused to sectioned brain tissue by crystal formation during freezing.
Animals ; Brain* ; Eosine Yellowish-(YS) ; Freezing ; Hematoxylin ; Immunohistochemistry ; Mice ; Sucrose

OBJECTIVE: To compare black rice (Oryza sativa L) extract with three different staining methods for human sperm head assessment. METHODS: Semen samples were collected from 34 volunteers. Four smears of each ejaculate were prepared for staining using the rapid Papanicolaou (PAP) stain, SpermBlue, DipQuick, and black rice extract. The percentage of defective sperm heads (mean±standard deviation) was compared. RESULTS: Black glutinous rice extract, a natural dye, was used instead of hematoxylin to stain the nuclei of the sperm heads. The percentage of defective sperm heads showed a significant difference between black rice extract and DipQuick (p=0.000). In contrast, black rice extract and rapid PAP showed no statistically significant difference (p=0.974). A strong correlation (r =0.761) was found between the findings obtained using rapid PAP and black rice extract. In contrast, a weak correlation (r =0.248) was obtained between DipQuick and black rice extract for the percentage of defective sperm heads. CONCLUSION: The results showed good agreement and a strong correlation between the rapid PAP and black rice extract stains. The advantages of black rice extract as a novel substitute for hematoxylin for nuclear staining include ease of preparation, local availability, and favorable nuclear staining properties. Further studies could also focus on comparing staining techniques in clinical samples.
Coloring Agents ; Hematoxylin ; Humans ; Semen ; Sperm Head ; Spermatozoa ; Volunteers

PURPOSE: The purpose of this study is to determine the efficacy of bioactive hydroxyapatite obtained from the porcine cancellous bone for the treatment of bone defect and nonunion. MATERIALS AND METHODS: Porcine cancellous bones were heat-treated at 1300degrees C for 2 hours. The chemical compositions, calcium to phosphate ratio and microstructures of mineralized porcine bone were examined. For in vivo implantation, bone defects were made on the anteromedial aspects of proximal tibia in 7 beagle dogs and theses artificial bones were inserted. Plain X-ray was taken at every 2 weeks interval for radiologic evaluation. At 12 weeks, specimens were evaluated histologyically with hematoxylin and eosin stain. RESULTS: The composition and morphology of mineralized porcine cancellous bone were similar to those of heat-treated human cancellous bone. Radiographs showed union at the host bone-bone block interfaces. At 12 weeks, all uniform and substantial new bone formation were observed. CONCLUSION: This mineralization technique has several advantages such as no disease transmission, no immune reaction, excellent biocompatibility, and cost-effectiveness. Consequently, mineralized porcine cancellous bone showed an effective osteoconductivity.
Animals ; Calcium ; Dogs ; Durapatite ; Eosine Yellowish-(YS) ; Hematoxylin ; Humans ; Osteogenesis ; Tibia

Since the culture of M. furfur is impossible, the KOH wet mount and various staining techniques have been applied for identification of the M. furfur. However, these methods still have many disputed points. Practically, the KOH wet mount method is in common use but. there are many difficulties in identifying the fungi. The author intended to suggest an easy and simple method for identification of the fungi, using the KOH and various other staining solutions, and comparing this with many known methods. At the same time, by applying the best method of identification which the author was able to develop, distribution of the fungi in the horny layer and the viability of the fungi during treatment were abserved In identifying the fungi, 1% toluidine blue was most excellent, but hematoxylin, eosin, cotton blue, Giemsa stain, and Wright stain were not so satisfactory. 2. After staining with l% toluidine blue to the skin lesion scotch tape was applied to the lesion briefly and then examined under direct microscope. This was most easy and convenient method. 3. Repeated scotch taping from ] to 12 times produced no change in the distribution of fungi in the horny layer, but after 28 applications there was remarkable reduction of the amount of the fungi and no fungi was demonstrated in groups taped more than 46 times. 4. No influence was noted in the distribution of fungi after repeated irradiation of Ultra-violet light once daily for 18 days. 5. Continous daily application of 25% sodium thiosulfate solution for average 3. 9 days, caused the disappearance of tungi and no fungal elements were found following EO days of successive observation. 6. Application of 2.5% selenium sulfide on alternate day for average 6.2 days, caused the disappearance of fungi and no fungal elements were found following 55- 62 days of successive observation.
Azure Stains ; Eosine Yellowish-(YS) ; Fungi ; Hematoxylin ; Malassezia* ; Selenium ; Skin ; Sodium ; Tinea Versicolor* ; Tinea* ; Tolonium Chloride

BACKGROUND/AIMS: The rapid urease test (RUT) is an invasive method to diagnose Helicobacter pylori infection, which relies on the acquisition and examination of gastric antrum and body tissues. We determined and compared the efficacy of RUT when the tissues were examined separately or after being combined. METHODS: Two hundred and fourteen patients were included and underwent esophagogastroduodenoscopy from July 2008 to June 2010. The separate test was defined as evaluating the status of infectivity of H. pylori from the antrum and body separately; whereas the united test was carried out putting both tissues from the antrum and body in the same RUT kit. All RUTs were read by a single observer 1, 3, 6, 12, and up to 24 hours later. We also got two biopsy specimens stained with hematoxylin and eosin and quantified H. pylori density was calculated on a scale of 0 to 3. RESULTS: Overall positivity for H. pylori was 137 (64%) for the separate test and 148 (69.2%) for the united test (p<0.01). The mean time to a positive test was 3.58 hours for the separate test and 1.69 hours for the united test (p<0.01). The correlation between the time to positive RUT and the severity of histology showed r=+0.556 for the antrum (p<0.01) and r=+0.622 for the body (p<0.01). CONCLUSIONS: Combining tissues prior to RUT enhances the detection of H. pylori, as compared with the examination of separate tissues, and shortens the time to develop a positive reaction by approximately 50%. These diagnostic advantages are also accompanied by increased cost-savings.
Biopsy ; Endoscopy, Digestive System ; Eosine Yellowish-(YS) ; Helicobacter ; Helicobacter pylori ; Hematoxylin ; Humans ; Pyloric Antrum ; Urease