1.Comparison of Two Internet Based Telepathology Systems: CORBA and ActiveX System.
Byeong il LEE ; Heung Kook CHOI ; Byong Hwan SON ; Sang Hee NAM ; Nam Hoon CHO
Journal of Korean Society of Medical Informatics 2003;9(3):285-295
Telepathology systems will be common systems in hospitals. The two systems were designed and implemented in web environments for test. One was implemented with the Common Object Request Broker Architecture (CORBA) technique. The other system was implemented in the form of ActiveX. The histopathological materials were stained by Hematoxylin and Eosin. By the Donpisha CCD camera attached to an Olympus BX-51 optical microscope 180 color images come to be acquired. For evaluation of the systems, transmission times and telediagnosis concordance rates were measured. Image processing ability was tested using two telepathology systems. For the local area test, system I using CORBA had measured image transmission times of 0.1 s, 0.2 s, and 0.4 s at the file sizes of 100 K byte, 900 K byte and 3.6 M byte respectively. Transmission times for system II using Component Object Model (COM) were slightly slower, ranging from 0.02 s to 0.05 s. In the long distance area test, system II transmission times were 0.5 s, 0.8 s, and 2.0 s. The overall concordance rate of telediagnosis for the 180 images was 78.3%. In this study, we compared our systems about image transmission, and processing for the further development of system configurations.
Eosine Yellowish-(YS)
;
Hematoxylin
;
Internet*
;
Telepathology*
2.Improvement program on pretreatment of acid decalcified tissue in hematoxylin-eosin staining.
Li-Hong YAO ; Mei ZHANG ; Mei-Chang HUANG ; Zi-Xin WAN ; Wei-Long ZHANG ; Xiao YANG ; Ming-Zhong YANG ; Yu CHEN ; Ya-Ling TANG
West China Journal of Stomatology 2020;38(3):297-300
OBJECTIVE:
To explore the treatment conditions of acid decalcified specimens and improve the poor quality of sections and unclear structure of hematoxylin-eosin (HE) staining caused by the change in pH in tooth and hard tissue after acid decalcification.
METHODS:
A total of 20 cases of oral pathological specimens that contain hard tissues were decalcified and treated with routine treatment, concentrated ammonia water immersion treatment, and saturated lithium carbonate solution immersion treatment. The quality and HE staining effects of hard tissue sections treated with different methods were compared.
RESULTS:
Compared with routine treatment, lithium carbonate saturated solution treatment showed complete sections. Hematoxylin is strongly stained, the nucleus is clear, and the cytoplasm is bright.
CONCLUSIONS
Soaking acid decalcified specimens in lithium carbonate saturated solution before embedding in dehydration can neutralize the acidic environment of the tissue. The quality of sections and HE staining effect are improved and are suitable for the pretreatment of acid decalcified tissue samples of oral pathology.
Eosine Yellowish-(YS)
;
Hematoxylin
;
Staining and Labeling
;
Tooth
3.Mucoepidermoid Carcinoma of the Palpebral Conjunctiva.
Journal of the Korean Ophthalmological Society 2006;47(10):1669-1672
PURPOSE: We report our experience with a case of a nodular lesion of the lower palpebral conjunctiva with histologic features consistent with mucoepidermoid carcinoma. METHODS: A 72 year-old man presented with a left lower palpebral conjunctiva lesion. 3 x 3 mm sized flat pedunculated yellowish red mass was observed. Incisional biopsy proved the mass a mucoepidermoid carcinoma and wide local resection was conducted. RESULTS: Hematoxylin and eosin-stained sections showed lobules of tumor cells, consisting of predominantly epidermoid cells and scattered islands of mucin-secreting cells. Mucicarmine-stained sections demonstrated strong positivity, consistent with the diagnosis of mucoepidermoid carcinoma. CONCLUSIONS: In any case presenting palpebral conjunctival lesion, mucoepidermoid carcinoma should be differentiated, and it is necessary to confirm the diagnosis through biopsy.
Aged
;
Biopsy
;
Carcinoma, Mucoepidermoid*
;
Conjunctiva*
;
Diagnosis
;
Hematoxylin
;
Humans
;
Islands
4.A Study on the Clinical and Histopathologic Classification of Leprosy.
Korean Journal of Dermatology 1980;18(6):523-528
For comparison of clinical classification of leprosy to histopathologic classification, a detailed histopathologic study, using hematoxylin and eosin stain and Ziehl-Neelsen stain, was done on 72 fresh uncomplicated cases of leprosy. The clinical classification was done using the criteria of Ridley and Jopling (1966), and the microscopic features were classified according to Ridley's(1974) definition. Clinical classification revealed that 8 of total 72 patients had tuberculoid(TT), 9 had borderline tuberculoid (BT), 5 had borderline(BB), 10 had borderline lepromatous.(BL), and 31 had lepromatous leprosy(LL). Nine patients were claasified as indeterminate(I) group. Histopathologic classification showed that 3 cases presented tuberculoid(TT), 10 presented borderline tuberculoid(BT), 4 presented borderline(BB), 9 presented borderline lepromatous(BL), 20 presented subpolar leprornatous(LLs), and 10 presented polar lepromatous(LLp) histopathologic characteristics, Sixteen cases were classified as indeterminate(I) leprosy by histopathologic findings. On comparison of clinical classification to histopathologic classification, the two were in consonance with each other in 50 cases(69.4%) and the disparity between them was noticed in 22 cases(30.6%). Among the 22 cases which showed disparity, there was a shift of one step either towards the tuberculoid or lepromatous end of the spectrum in 15 cases, and ihe remaining 7 cases were classified as indeterminate group beaause of nonspecific histopathologic changes.
Classification*
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Eosine Yellowish-(YS)
;
Hematoxylin
;
Humans
;
Leprosy*
;
Leprosy, Paucibacillary
5.Is optimal cutting temperature compound essential embedding solution treatment to cryo-sectioning of brain tissue?.
Hye Kyung BAEK ; Ji Ae SONG ; Sun Shin YI
Korean Journal of Veterinary Research 2016;56(2):85-89
We tested a set of conditions for obtaining optimal tissue quality in preparation for histology in samples of mouse brain. C57BL/6J mice were sacrificed and perfused with 4% paraformaldehyde, after which the brains were removed and dehydrated in 30% sucrose solution. The brains were then divided into four groups according to freezing temperature and usage of optimal cutting temperature (OCT) compound. Next, we stained the sectioned brain tissues with Harris hematoxylin and eosin Y and immunohistochemistry was performed for doublecortin. The best quality tissue was obtained at -25℃ and by not embedding with the OCT compound. When frozen at -25℃, the embedded tissue was significantly damaged by crystals, while at -80℃ there were no meaningful differences between qualities of embedded- and non-embedded tissues. Overall, we identified a set of conditions to obtain quality frozen brain sections. Our developed protocol will help resolve matters associated with damage caused to sectioned brain tissue by crystal formation during freezing.
Animals
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Brain*
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Eosine Yellowish-(YS)
;
Freezing
;
Hematoxylin
;
Immunohistochemistry
;
Mice
;
Sucrose
6.Evaluation of black glutinous rice (Oryza sativa L) extract as a novel nuclear stain for human sperm head assessment by microscopic examination
Sirinart CHOMEAN ; Tanawan SUKANTO ; Arreya PIEMSUP ; Jirattikan CHAIYA ; Kolunya SAENGUTHAI ; Chollanot KASET
Clinical and Experimental Reproductive Medicine 2019;46(2):60-66
OBJECTIVE: To compare black rice (Oryza sativa L) extract with three different staining methods for human sperm head assessment. METHODS: Semen samples were collected from 34 volunteers. Four smears of each ejaculate were prepared for staining using the rapid Papanicolaou (PAP) stain, SpermBlue, DipQuick, and black rice extract. The percentage of defective sperm heads (mean±standard deviation) was compared. RESULTS: Black glutinous rice extract, a natural dye, was used instead of hematoxylin to stain the nuclei of the sperm heads. The percentage of defective sperm heads showed a significant difference between black rice extract and DipQuick (p=0.000). In contrast, black rice extract and rapid PAP showed no statistically significant difference (p=0.974). A strong correlation (r =0.761) was found between the findings obtained using rapid PAP and black rice extract. In contrast, a weak correlation (r =0.248) was obtained between DipQuick and black rice extract for the percentage of defective sperm heads. CONCLUSION: The results showed good agreement and a strong correlation between the rapid PAP and black rice extract stains. The advantages of black rice extract as a novel substitute for hematoxylin for nuclear staining include ease of preparation, local availability, and favorable nuclear staining properties. Further studies could also focus on comparing staining techniques in clinical samples.
Coloring Agents
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Hematoxylin
;
Humans
;
Semen
;
Sperm Head
;
Spermatozoa
;
Volunteers
8.Morphology and Adhesion Complex of Cultured Epithelium, on Amniotic Membrane in Vitro and in Vivo.
Yong Gen XU ; Si Hwan CHOI ; Seon Mi KO ; Yang Won LEE ; Moo Byung CHA
Journal of the Korean Ophthalmological Society 2006;47(1):160-170
PURPOSE: The morphologic characteristics and adhesion complex formation of cultured limbal epithelium of rabbit on amniotic membrane, in vitro and in vivo. METHODS: Rabbit limbal explants were cultured in vitro on amniotic membrane for 4 weeks. In the in vivo culture, the rabbit corneal epithelium was removed. Next, a tunnel was created at the limbus and, the edge of amniotic membrane was secured in the tunnel and cultured for 4 weeks. The proliferation of epithelium on the amniotic membrane was observed for 4 weeks at 1 week intervals. RESULTS: AE-5 immunohistochemical staining was positive and PAS staining was negative for cultured rabbit limbal epithelium, in vitro and in vivo. Hematoxylin and Eosin staining showed the morphologic characteristics of normal rabbit corneal epithelium in both groups. Transmission electron microscopy performed at an interval of 1 week showed adhesion complex by 3 weeks of in vitro culture, and no significant change was seen until week 4. The formation of the adhesion complex was shown starting at week 1 of in vivo culture and increased until week 4. CONCLUSIONS: The morphology of corneal limbal epithelium of rabbits cultured on amniotic membrane in vitro and in vivo, did not differ significantly compared with normal rabbit epithelium. In vivo culture resulted in more a normal-looking adhesion complex compared with the in vitro culture.
Amnion*
;
Eosine Yellowish-(YS)
;
Epithelium*
;
Epithelium, Corneal
;
Hematoxylin
;
Microscopy, Electron, Transmission
;
Rabbits
9.Morphologic Changes in Cultured Normal Human Nasal Epithelial Cells Following Treatment with Histamine.
Kyung Su KIM ; Joo Heon YOON ; Jeung Gweon LEE ; Hyoung Jin MOON ; Hee Sun CHUN ; Shin Young YOO
Journal of Rhinology 1999;6(1):12-18
The aims of this study are to observe morphologic changes in normal human nasal epithelial (NHNE) cells caused by varying concentrations of histamine, to evaluate the changes in relation to the degree of epithelial differentiation, and to examine whether these changes are caused by the proper action of histamine or are general inflammatory processes represented by lipopolysaccharide (LPS). Cultured NHNE cells were treated with histamine diphosphate and LPS 0111 : B4 at different concentrations : 20 ng/ml, 200 ng/ml, 2 microgram/ml and 20 microgram/ml. The timing of the treatment was conducted in one of two ways : a duration of 48 hours on floating day 12 or a duration of seven days on floating day seven. On floating day 14, all specimens were collected, and hematoxylin & eosin staining and scanning electron microscopy (SEM) were conducted. The areas of ciliated and secretory cell were calculated with the Optimas program. In SEM of specimens that were given 48-hour treatments of histamine and LPS at 20 microgram/ml dosages, coverage by secretory cells had increased and damaged cilia were noted. In SEM of specimens given the seven-day treatment, enlargement of the secretory cell area and damaged cilia were observed after a treatment of 20 microgram/ml LPS, but in specimens treated with histamine, findings were normal. Morphologic changes caused by histamine treatment may be a nonspecific finding observed in inflammation, and the changes can vary according to the differentiation of the epithelium.
Cilia
;
Eosine Yellowish-(YS)
;
Epithelial Cells*
;
Epithelium
;
Hematoxylin
;
Histamine*
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Humans*
;
Inflammation
;
Microscopy, Electron, Scanning
10.A Case of Nodular Amyloidosis.
Seok Ki JUNG ; Seung Ho CHANG ; Tae Young YOON
Annals of Dermatology 1999;11(1):47-50
A 38-year-old male patient visited our clinic complaining of three skin lesions on the scalp. There were yellowish to brownish, waxy, non-tender, walnut-sized nodules. Hematoxylin and eosin staining revealed amorphous pinkish material deposits in the dermis. The Congo red stain and Dylon stain under polarizing microscopy showed yellow-green birefringence and the immunoglobulin-lambda light chain stain showed a positive reaction. An electron microscopic examination revealed filaments with uniform diameter(6 to 10nm) that were straight and neither branched nor anastomosed. Based on the clinical, histopathological, immunohistochemical and electron microscopical findings, the skin lesions were diagnosed as nodular amyloidosis.
Adult
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Amyloidosis*
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Birefringence
;
Congo Red
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Dermis
;
Eosine Yellowish-(YS)
;
Hematoxylin
;
Humans
;
Male
;
Microscopy
;
Scalp
;
Skin