OBJECTIVETo investigate the effect of HMME-PDT (Hematoporphyrin Monomethyl Ether-Photodynamic therapy) on Hyperplastic scar in the rabbit ear.

METHODSThe acute model of dermal Hyperplastic scar in the rabbit ear was established. 24 scars were randomly divided into 2 groups: the experimental group (n = 12) received HMME-PDT treatment, and the controlled group (n = 12) received no special treatment. Specimens were harvested from scars on postoperative 28 day. Scar hyper plasty and collagen fibers were observed by haematoxylin-eosin staining and Van-Gieson staining respectively. The microvessel density was calculated under microscope.

RESULTSCompared with the controlled group, HMME-PDT treatment in the experimental group reduced scar formation, decreased the microvessel density and prevented excess collagen deposition at the wound site.

CONCLUSIONSHMME-PDT may play a role in inhibiting hyperplastic scar in rabbit ear.

Animals ; Cicatrix, Hypertrophic ; pathology ; therapy ; Ear ; pathology ; Female ; Hematoporphyrins ; pharmacology ; Male ; Photochemotherapy ; Rabbits

The mutagenic effect of HpD on cell SCE and the reactions of cell SCE to different sources of light combined with HpD were studied using V79 cells. There were 6 doses of HpD: 1 microgram/ml, 3 micrograms/ml, 5 micrograms/ml, 10 micrograms/ml, 50 micrograms/ml and 100 micrograms/ml. The dose of 5 micrograms/ml is equal to the maximum dose of HpD used in the clinic (HpD per milliliter of patient's blood). Our experiments demonstrated that when the cells were cultured in the dark and HpD was added to the medium no more than 5 micrograms/ml, the SCE frequencies were not increased. The cells were irradiated with different sources of light without HpD, both the fluorescence and ultraviolet light could promote SCE but the light of daylight lamp and red light did not increase it. But when HpD was added into culture medium at the dose of less than 5 micrograms/ml, every light could increase the cell SCE intensively except the daylight lamp light. The red light was more notable than the others by relation analysis.
Cells, Cultured ; Fluorescence ; Hematoporphyrin Photoradiation ; Hematoporphyrins ; pharmacology ; Humans ; Light ; Photochemotherapy ; Sister Chromatid Exchange ; drug effects ; Ultraviolet Rays

OBJECTIVETo investigate the killing effect of photodynamic therapy (PDT) mediated by hematoporphyrin derivative (HpD) on human colon carcinoma LoVo and CoLo205 cells in vitro.

METHODSLoVo and CoLo205 cells cultured in vitro were incubated in the presence of 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, and 4.0 microg/ml HpD for 4 h and exposed to different light doses delivered using a semiconductor laser at 630 nm with the energy density of 2, 5, 10, and 20 J/cm(2). After further culture for 24 h, the survival rate of LoVo and CoLo205 cells were analyzed by MTT assay, and the cellular fluorescence intensities of HpD were measured with a luminescence spectrometer.

RESULTSHpD-PDT resulted in effective cell killing to a comparable magnitude in LoVo and CoLo205 cells cultured in vitro (P>0.05). The killing effects were positively correlated with the concentration of HpD and the dosage of laser irradiation. Exposure to 20 J/cm(2) resulted in an IC(50) of LoVo and CoLo205 cells of 0.4 and 0.6 microg/ml respectively, which were not significantly different (P>0.05). The cellular HpD fluorescence intensities were also similar between the two cells.

CONCLUSIONHpD-PDT may effectively kill LoVo and CoLo205 cells cultured in vitro.

Animals ; Cell Line, Tumor ; Cell Survival ; drug effects ; radiation effects ; Colonic Neoplasms ; drug therapy ; pathology ; Dose-Response Relationship, Radiation ; Hematoporphyrins ; chemistry ; pharmacology ; Humans ; Lasers ; Photochemotherapy ; methods ; Photosensitizing Agents ; chemistry ; pharmacology ; Spectrometry, Fluorescence

OBJECTIVETo prepare photoimmunoconjugate of hematoporphyrin (HP) and herceptin, and study its killing and apoptosis-inducing effect on tumor cells BT-474.

METHODSHP-herceptin photoimmunoconjugate was synthesized with EDCI as the condensator. After exposure of the cells to 630 nm laser, the killing effect of the conjugate and cell apoptosis were evaluated by MTT assay and flow cytometry.

RESULTSCompared with free HP at equivalent dose, the immune reactivity, killing effect and the apoptosis-inducing effect of HP-herceptin immunoconjugate on BT-474 cells was enhanced (P<0.05).

CONCLUSIONThe killing effect of HP-herceptin immunoconjugate is stronger than free HP on BT-474 cells.

Antibodies, Monoclonal ; chemistry ; pharmacology ; Antibodies, Monoclonal, Humanized ; Antineoplastic Agents ; chemistry ; pharmacology ; Apoptosis ; drug effects ; Cell Line, Tumor ; Cell Survival ; drug effects ; Drug Compounding ; methods ; Flow Cytometry ; Hematoporphyrin Photoradiation ; methods ; Hematoporphyrins ; chemistry ; pharmacology ; Humans ; Immunoconjugates ; chemistry ; pharmacology ; Immunotherapy ; methods ; Photosensitizing Agents ; chemistry ; pharmacology ; Trastuzumab

Animal ; Antineoplastic Agents, Phytogenic/isolation & purification/*pharmacology ; Chlorophyll/*analogs & derivatives/isolation & purification/pharmacology/radiation effects ; Comparative Study ; Drug Screening Assays, Antitumor ; Feces/*chemistry ; Hematoporphyrin Derivative ; Hematoporphyrins/pharmacology ; Human ; Leukocytes, Mononuclear/drug effects ; Mice ; Oxygen/metabolism ; Photochemistry ; Photochemotherapy ; Radiation-Sensitizing Agents/isolation & purification/*pharmacology ; Silkworms/*metabolism ; Singlet Oxygen ; Spectrophotometry ; Structure-Activity Relationship ; Support, Non-U.S. Gov't ; Tumor Cells, Cultured/*drug effects/radiation effects