1.Main factors affecting the effect of photodynamic therapy against human esophageal cancer cells in vitro.
Xiao-hua CHEN ; Rong-cheng LUO ; Li-bo LI ; Xue-mei DING ; Cheng-wei LÜ ; Xiao-ping ZHOU ; Xiao YAN
Journal of Southern Medical University 2007;27(12):1817-1820
OBJECTIVETo evaluate the tumor cell-killing effect of photodynamic therapy against human esophageal cancer cells in vitro and identify the main factors affecting the effect.
METHODSHuman esophageal cancer Eca-109 cells were incubated for 24 h in vitro with hematoporphyrin derivative (HpD) and Photofrin at different concentrations prior to exposure to a light energy density of 15 J/cm(2) delivered from a DIOMED 630 PDT system. The cell killing effect was also evaluated for different HpD concentrations combined with 3 light energy densities (10, 30, and 50 J/cm(2)), respectively. The cell survival rate was measured using MTT assay, and fluorescence spectrometry was used to detect the intracellular photosensitizer fluorescence of the tumor cells after incubation with HpD for 4 h.
RESULTSThe cell survival rate after incubation with the two photosensitizers at different concentrations were significantly different, and under the 3 different light energy densities, incubation of the cells with different HpD concentrations also resulted in significantly different cell survival rates (P<0.05). At the 4 low photosensitizer concentrations and with different light energy densities, the cell survival rates were similar (P>0.05), but the 4 higher photosensitizer concentrations resulted in significant difference in the cells survival (P<0.05). Correlation analysis showed that the intracellular photosensitizer concentration was positively correlated to the photosensitizer concentrations in cell incubation (r=0.997).
CONCLUSIONWhen the light source remains constant, the light energy density, the kinds of photosensitizers and their concentrations are the main factors affecting the Eca-109 cell-killing effect of PDT.
Cell Line, Tumor ; Cell Survival ; Dihematoporphyrin Ether ; pharmacology ; Esophageal Neoplasms ; drug therapy ; Hematoporphyrin Derivative ; pharmacology ; Hematoporphyrin Photoradiation ; Humans ; Light ; Photosensitizing Agents ; pharmacology
2.Clinical Experiences of Photodynamic Therapy in the Extramammary Paget's Disease.
Hang Wook CHANG ; Seung Chul BAEK ; Dae Gyoo BYUN ; Dong HOUH
Korean Journal of Dermatology 2001;39(4):446-453
BACKGROUND: Photodynamic therapy(PDT) is a kind of non-invasive photochemotherapy, which is designed to kill the target cells through photodynamic reaction. It has been reported that PDT is very effective in superficial lesions, including extramammary Paget's disease. Yet, there has been no clinical trial for the treatment of extramammary Paget's disease using PDT in Korea. OBJECTIVE: The purpose of this study was to evaluate the effectiveness of PDT in treating extramammary Paget's disease METHOD: Seven patients with anogenital extramammary Paget's disease were treated repeatedly with local PDT using topical ALA 20% and intralesional injection of hematoporphyrin derivative (HpD), and systemic PDT using HpD. Fluences of 125-200J/cm2 and intensities of 50-100mW/cm2 were used. RESULT: The clinical outcomes were disappointing. All the patients experienced only partial responses despite repeated treatments. But side effects were minimal. CONCLUSION: PDT is not effective enough for the treatment of extramammary Paget's disease to be used as a primary therapy. It would be better to use as an adjuvant to other treatment methods. Photodynamic sensitization, also known as photodynamic diagnosis, might help us define a more reliable border of the extent involved and the surgical margin to be excised.
Diagnosis
;
Hematoporphyrin Derivative
;
Humans
;
Injections, Intralesional
;
Korea
;
Paget Disease, Extramammary*
;
Photochemotherapy*
3.Clinical Experiences of Photodynamic Therapy in the Extramammary Paget's Disease.
Hang Wook CHANG ; Seung Chul BAEK ; Dae Gyoo BYUN ; Dong HOUH
Korean Journal of Dermatology 2001;39(4):446-453
BACKGROUND: Photodynamic therapy(PDT) is a kind of non-invasive photochemotherapy, which is designed to kill the target cells through photodynamic reaction. It has been reported that PDT is very effective in superficial lesions, including extramammary Paget's disease. Yet, there has been no clinical trial for the treatment of extramammary Paget's disease using PDT in Korea. OBJECTIVE: The purpose of this study was to evaluate the effectiveness of PDT in treating extramammary Paget's disease METHOD: Seven patients with anogenital extramammary Paget's disease were treated repeatedly with local PDT using topical ALA 20% and intralesional injection of hematoporphyrin derivative (HpD), and systemic PDT using HpD. Fluences of 125-200J/cm2 and intensities of 50-100mW/cm2 were used. RESULT: The clinical outcomes were disappointing. All the patients experienced only partial responses despite repeated treatments. But side effects were minimal. CONCLUSION: PDT is not effective enough for the treatment of extramammary Paget's disease to be used as a primary therapy. It would be better to use as an adjuvant to other treatment methods. Photodynamic sensitization, also known as photodynamic diagnosis, might help us define a more reliable border of the extent involved and the surgical margin to be excised.
Diagnosis
;
Hematoporphyrin Derivative
;
Humans
;
Injections, Intralesional
;
Korea
;
Paget Disease, Extramammary*
;
Photochemotherapy*
4.Photodynamic therapy and its application in gynecologic oncology.
Acta Academiae Medicinae Sinicae 2003;25(4):484-486
While photodynamic therapy is applied on neoplasm, photosensitisers tend to accumulate in neoplastic tissues. With appropriate wavelength light, it causes photochemical reaction and destructs neoplastic tissues. Its better selection for tumor tissue with effective photochemical reaction, and lower side effect make it widespread application in gynecologic oncology. At present, photodynamic therapy has been used in diagnosing and treating lower genital tract carcinoma in situ, and advanced malignant tumor such as vulval and ovarian carcinoma.
Female
;
Genital Neoplasms, Female
;
drug therapy
;
Hematoporphyrin Derivative
;
therapeutic use
;
Hematoporphyrin Photoradiation
;
Humans
;
Ovarian Neoplasms
;
drug therapy
;
Photochemotherapy
;
Photosensitizing Agents
;
therapeutic use
;
Uterine Cervical Neoplasms
;
drug therapy
5.Research progress of the anti-tumor effect of sonodynamic and photodynamic therapy.
Xiaomin SU ; Long LI ; Pan WANG
Journal of Biomedical Engineering 2012;29(3):583-587
Cancer, as a serious threat to human health, is one of the major killers. The treatment of cancer has attracted more and more attention. Currently, the means of treating cancer is also increasing, but there is no emergence of a fully satisfactory treatment. A combination of sonodynamic therapy (SDT) and photodynamic therapy (PDT), named sono-photodynamic therapy (S-PDT), is a new composite cancer therapy. Because the therapy can significantly improve the tumor curing effect, it has good application prospects in cancer prevention and treatment. The present article reviewed the progress of the anti-tumor mechanisms and influencing factors of S-PDT.
Animals
;
Antineoplastic Agents
;
administration & dosage
;
Combined Modality Therapy
;
Hematoporphyrin Derivative
;
administration & dosage
;
Hematoporphyrin Photoradiation
;
Humans
;
Neoplasms
;
drug therapy
;
therapy
;
Photochemotherapy
;
methods
;
Photosensitizing Agents
;
administration & dosage
;
Ultrasonic Therapy
;
methods
6.Experimental interstitial photodynamic therapy of the Morris 7777 hepatoma in the rat.
Young Koog CHEON ; Young Seok KIM ; Young Deok CHO ; Yun Soo KIM ; Joon Seong LEE ; Moon Sung LEE ; Chan Sup SHIM ; Boo Sung KIM ; So Young JIN
Korean Journal of Medicine 2004;66(4):397-403
BACKGROUND: Photodynamic therapy (PDT) of hepatic tumors has been restricted due to the preferential retention of photosensitizers in normal liver tissue. Therefore, superficial tumor illumination causes subsequential liver necrosis. Moreover, the limited light penetration during superficial illumination makes it impossible to treat deep-seated or larger solid tumors. These limitations were overcome by interstitial therapy. Aim: The aim of study is to investigate the macroscopic and microscopic effect of a single session of interstitial photodynamic therapy on liver tumor. METHODS: The Morris 7777 hepatoma cells were injected subcutaneously into the flanks of Buffalo female rats. Tumor growth was monitored with measuring the tumor size. Animals were pretreated with hematoporphyrin derivative 48 hours prior to the intratumoral delivery of laser radiation, when the tumor had reached a volume greater than 1.0 cm3. One or two weeks after interstitial PDT, the extent of tumor necrosis was evaluated microscopically. RESULTS: Volume of lesions averaged 10.2 mm3. Histologic stains demonstrated microvascular thrombosis and coagulative necrosis within the lesions. There appeared to be 100% cellular destruction within the lesion by cytochemical staining. CONCLUSION: The results of this study show the ability of interstitial PDT to cause destruction of liver tumor.
Animals
;
Buffaloes
;
Carcinoma, Hepatocellular*
;
Coloring Agents
;
Female
;
Hematoporphyrin Derivative
;
Humans
;
Lighting
;
Liver
;
Necrosis
;
Photochemotherapy*
;
Photosensitizing Agents
;
Rats*
;
Thrombosis
7.Photodynamic Therapy for Superficial Gastrointestinal Tumors.
Young Koog CHEON ; Dae Hee HAN ; In Seop JUNG ; Chang Beom RYU ; Joo Young CHO ; Yun Soo KIM ; Joon Seong LEE ; Moon Sung LEE ; Chan Sup SHIM ; Boo Sung KIM
Korean Journal of Gastrointestinal Endoscopy 2006;32(2):81-86
BACKGROUND/AIMS: Although photodynamic therapy (PDT) has been used for the endoscopic treatment of digestive cancer, its curative efficacy remains uncertain. This study evaluated the curative role of PDT in superficial gastrointestinal cancer. METHODS: Fifteen lesions in 14 patients with a histologically proven carcinoma (early esophageal cancer 6, early gastric cancer 8, ampulla of Vater cancer 1) were injected with an intravenous hematoporphyrin derivative (2 mg/kg), and PDT was performed 48 hours later. The response to treatment was assessed by gastroscopy with biopsies. RESULTS: The median follow-up time was 273 days (42~1,030 days). According to the TNM stage of endoscopic ultrasonography, there were 14 T1 cases and 1 T2 case. Complete remission was observed in 13 cases after the initial and consecutive PDT. There were 2 cases of failure. The recurrence rate was 15.4% (2/13), and the median time from the initial PDT to recurrence was 349 days. CONCLUSIONS: PDT using a hematoporphyrin derivative as a photosensitizer is a safe and efficient method for treating early cancer. However, a long-term follow up period using a large population sample will be needed for confirmation.
Ampulla of Vater
;
Biopsy
;
Endosonography
;
Esophageal Neoplasms
;
Follow-Up Studies
;
Gastrointestinal Neoplasms
;
Gastroscopy
;
Hematoporphyrin Derivative
;
Humans
;
Photochemotherapy*
;
Recurrence
;
Stomach Neoplasms
8.A Case of Bowen's Disease Partially Responded to Photodynamic Therapy.
Si Heon LEE ; Byung Cheol JUNG ; Min Jung WOO ; Dong Seok KIM ; Sang Won KIM
Annals of Dermatology 2002;14(1):38-41
Photodynamic therapy(PDT) is a treatment modality by highly reactive oxygen intermediates generated through the interaction of light with a photosensiziter. It has been shown to be an effective treatment for various cutaneous and noncutaneous malignancies. It is efficient for the curative and palliative treatment of epithelial skin tumor in situ or early invasive lesions. In effect, it is a useful alternative treatment for the lesions located on anatomically difficult areas or the large-sized lesions. We treated a case of Bowen's disease arising on the plantar area and 3rd and 4th toewebs of left forefoot in a 61-year-old man with PDT using the hematoporphyrin derivative, porfirmer sodium(Photofrin, Russia) as a photosensitizer and gold vapor laser as a visible light source. The outcome showed partial clinical improvement after about 2 months' follow-up.
Bowen's Disease*
;
Follow-Up Studies
;
Hematoporphyrin Derivative
;
Humans
;
Lasers, Gas
;
Light
;
Middle Aged
;
Oxygen
;
Palliative Care
;
Photochemotherapy*
;
Skin
9.Hematoporphyrin derivative-mediated photodynamic therapy for human nasopharyngeal carcinoma: a comparative study with CNE2 and C666-1 cell lines in vitro.
Xiao-Min YANG ; Rong-Cheng LUO ; Hong-Jing MA ; Li-Bo LI ; Xue-Mei DING ; Xiao YAN ; Cheng-Wei LÜ ; Xiao-Ping ZHOU
Journal of Southern Medical University 2007;27(2):165-167
OBJECTIVETo investigate biological effect of hematoporphyrin derivative (HpD) photodynamic therapy (PDT) on in vitro cultured nasopharyngeal carcinoma (NPC) cell lines CNE2 and C666-1.
METHODSCNE2 and C666-1 cells cultured in vitro were incubated in a medium containing HpD at different concentrations (0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, and 4.0 microg/ml) for 4 h followed by exposure to different light doses (2, 5, 10, and 20 J/cm2) using a diode laser at 630 nm with power density of 20 mW/cm2. After 24 h of incubation with HpD-PDT, the survival rate of CNE2 and C666-1 cells were analyzed by MTT assay.
RESULTSHpD-PDT produced effective killing of CNE2 and C666-1 cells cultured in vitro, and the killing effects were positively correlated with HpD concentration and the irradiation dose. Exposure of CNE2 and C666-1 cells to irradiation dose of 20 J/cm2 resulted in the IC50 of 0.7 and 1.2 microg/ml, respectively (P<0.01). With the same HpD concentration and irradiation dose, the survival rate of C666-1 cells, however, was significantly higher than that of CNE2 cells (P<0.05).
CONCLUSIONHpD-PDT may result in effective killing of CNE2 and C666-1 cells cultured in vitro, although C666-1 cells are less sensitive to HpD-PDT than CNE2 cells.
Antineoplastic Agents ; pharmacology ; Cell Line, Tumor ; Cell Survival ; drug effects ; radiation effects ; Dose-Response Relationship, Drug ; Dose-Response Relationship, Radiation ; Hematoporphyrin Derivative ; pharmacology ; Hematoporphyrin Photoradiation ; methods ; Humans ; Nasopharyngeal Neoplasms ; pathology ; Photochemotherapy ; methods ; Photosensitizing Agents ; pharmacology
10.Mechanism of photodynamic therapy against human esophageal carcinoma xenografts in nude mice.
Xiao-Hua CHEN ; Rong-Cheng LUO ; Li-Bo LI ; Xue-Mei DING ; Cheng-Wei LV ; Xiao-Ping ZHOU ; Xiao YAN
Journal of Southern Medical University 2009;29(11):2222-2224
OBJECTIVETo investigate the mechanism of photodynamic therapy (PDT) in nude mice bearing human esophageal cancer cell line Eca-109 xenografts.
METHODSA nude mouse model bearing human esophageal carcinoma was established by subcutaneous transplantation of Eca-109 cells. The mice were then randomized into 4 groups, namely hematoporphyrin derivative (HpD)-PDT group (given HpD and laser irradiation), exclusive laser irradiation group, exclusive HpD group and blank control group. In HpD-PDT group, the mice were exposed to irradiation at the light energy density of 120 Jsol;cm(2) delivered via a DIOMED 630 PDT system 24 h after intraperitoneal HpD injection, and the mice in exclusive laser irradiation group received only laser irradiation. Three days later, all the nude mice were sacrificed for determination of malondialdehyde (MDA) production, immunohistochemistry for caspase-3 protein and HE staining of the tumor tissue.
RESULTSThe MDA level was significantly higher in HpD-PDT group than in the other 3 groups (P<0.01), and comparable between the latter 3 groups. Expression of caspase-3 protein was similar between HpD-PDT group and the blank control group (P>0.05). Under light microscope, HE staining visualized massive tissue necrosis in HpD-PDT group with homogeneous red staining.
CONCLUSIONIn human esophageal carcinoma xenografts in nude mice, HpD-PDT generates singlet oxygen to result in direct tumor cell damage and cause MDA production. Caspase-3 may not be activated in the apoptotic pathway, suggesting that this pathway may not be caspase-3-dependent.
Animals ; Cell Line, Tumor ; Esophageal Neoplasms ; drug therapy ; pathology ; Female ; Hematoporphyrin Derivative ; therapeutic use ; Humans ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Neoplasm Transplantation ; Photochemotherapy ; Random Allocation