No abstract available.
Hematopoietic Stem Cell Mobilization* ; Hematopoietic Stem Cells*

No abstract available.
Hematopoietic Stem Cell Mobilization ; Heterocyclic Compounds ; Korea ; Stem Cells

Hematopoietic Stem Cell Transplantation ; Granulocyte Colony-Stimulating Factor/therapeutic use* ; Immunoglobulins ; Granulocytes ; Hematopoietic Stem Cell Mobilization

Granulocyte Colony-Stimulating Factor ; Hematopoietic Stem Cell Mobilization ; Hematopoietic Stem Cell Transplantation ; Hematopoietic Stem Cells ; Transplantation, Autologous

Animals ; Embryonic Stem Cells ; transplantation ; Hematopoietic Stem Cell Mobilization ; Humans ; Myocardium ; cytology ; Stem Cell Transplantation

BACKGROUND: Peripheral hematopoietic stem cell mobilization is increasing due to its advantages. For successful engraftment, obtaining sufficient stem cells is prerequisite. The number of CD34+ cells of collected blood are widely used to predict the engraftment potential. To determine the optimal point for collection of peripheral blood stem cell (PBSC), enumeration of the number of CD34+ cells in peripheral blood (PB) is known to be helpful. The purpose of this study is to analyze cutoff value of CD34+ cells in PB. METHODS: We analyzed 407 cases of autologous PBSC collection and 107 cases of allogenic PBSC collection during 2004~2009 in Pusan National University Hospital. Complete blood count, HPC fraction and number, CD34+ cells in PB and product of PBSC collection were analyzed. RESULTS: The each number of mononuclear cells and HPC in PB showed a strong correlation with CD34+ cells in PB. A strong correlation between the number of circulation CD34+ cells in PB on the day of collection and the number of collected CD34+ cells was found. The ROC curve revealed that the cutoff point having the optimal sensitivity and specificity at 8.5/uL for target CD34+ cells > or =1.0x10(6)/kg, 10.5/uL for target CD34+ cells > or =1.5x10(6)/kg and 13.5/uL for target CD34+ cells > or =2.0x10(6)/kg in this study. CONCLUSION: To obtain a sufficient yield of CD34+ cells during PBSC collection, determination of cut off point for each target CD34+ cells//kg is helpful to decide the collection.
Blood Cell Count ; Hematopoietic Stem Cell Mobilization ; ROC Curve ; Sensitivity and Specificity ; Stem Cells

Hematopoietic Stem Cell Mobilization ; methods ; Humans ; Peripheral Blood Stem Cell Transplantation ; methods

OBJECTIVE: To analyze the factors influencing the mobilization of autologous peripheral blood stem cells (auto-PBSCs) in patients with lymphoma and multiple myeloma, and provide reference for optimizing the autologous stem cell mobilization regimen. METHODS: Clinical data of 33 multiple myeloma and lymphoma patients received auto-PBSCs mobilization in our center from January 2015 to December 2018 were collected, the correlation of mobilization failure rate with gender, age, courses of chemotherapy before mobilization, does of recombinant human granulocyte colony stimulating factor (rhG-CSF), type of disease, and chemotherapy regimen were retrospectively analyzed. RESULTS: Type of disease and course of pre-mobilization chemotherapy could affect the mobilization failure rate (P<0.05). The mobilization failure rate of lymphoma patients was 42.1%, which was significantly higher than 7.1% of multiple myeloma patients (P=0.026). The mobilization failure rate was higher in the group with chemotherapy courses≥5 before mobilization (P=0.016). Age, gender, dose of rhG-CSF, and chemotherapy regimen had no significant correlation with mobilization failure rate (P>0.05). CONCLUSION Multi-course chemotherapy before collection and lymphoma patients are poor factors negatively impacting on auto-PBSCs mobilization.
Hematopoietic Stem Cell Mobilization ; Humans ; Lymphoma/therapy* ; Multiple Myeloma/therapy* ; Peripheral Blood Stem Cells ; Retrospective Studies

In the absence of significant improvement of disease-free survival (DFS) and overall survival (OS) by conventional chemotherapy, high dose chemotherapy in combination with hematopoietic stem cell transplantation has been increasingly used in the past decade for multiple myeloma (MM). Autologous stem cell transplantation (ASCT) was one of the most widely used methods in the treatment of MM. The patients who received ASCT may achieve a very good remission rate. ASCT may improve DFS but its application in clinic was limited by its high relapse rate. Tandem transplantation was feasible by its significant improvement of complete remission, but needed further evaluation. Allogeneic stem cell transplantation (Allo-SCT) has graft-versus-myeloma (GVM) effect, and provides molecular remission in about one third patients. It can only be offered to a small proportion of patients because of its high transplant-related mortality (TRM). Non-myeloablative transplantation is an attractive alternative tested currently in frontline treatment because of its GVM effect and low TRM.
Hematopoietic Stem Cell Mobilization ; Hematopoietic Stem Cell Transplantation ; methods ; Humans ; Multiple Myeloma ; therapy ; Prognosis ; Transplantation, Autologous ; Transplantation, Homologous

The aim of this study was to explore a safe method collecting peripheral blood stem/progenitor cell (PBSPC) from the infants of body weight less than 20 kg by using the COBE Spectra Blood Cell Separator through Auto-PBSC procedure. After washing tube by normal saline, one unit of irradiated RBC was infused into the apheresis set. When the collection terminated, only the concentrated RBC in the apheresis set was returned to the infant. The peripheral mononuclear cells (PBMNCs) and CD34+ cells were counted, the cell viability was determined. The results showed that 13 PBSPC collections were carried out successfully from 7 infants of body weight<20 kg. The average count of MNCs was 4.44x10(8)/kg [(3.46-6.45)x10(8)/kg], the CD34+ count was 2.20x10(6)/kg [(1.34-3.79)x10(6)/kg] and the cell viability was 98.45% (97%-100%) respectively. The vital signs of all the infants went smoothly during collection of PBSPCs. In conclusion, with the aid of COBE Spectra blood cell separator and other measures, the collection of PBSPCs from infants of body weight<20 kg is safe and effective, the PBMNCs containing enough PBSPC can be harvested for transplantation.
Cell Separation ; methods ; Child, Preschool ; Female ; Hematopoietic Stem Cell Mobilization ; methods ; Hematopoietic Stem Cells ; Humans ; Infant ; Male