2.Research of real-time fluorescent PCR in the rapid differential detection of H5, H9, H7 subtype avian influenza inactivated vaccines.
Jian-Feng HAN ; Yi-Bao NING ; Li SONG ; Cheng-Huai YANG
Chinese Journal of Biotechnology 2007;23(5):953-957
Specific primers and TaqMan MGB probes were designed with Primer Express 2.0 software according to the conserved region of the H5, H9, H7 subtype AIV hemagglutinin gene to make research of real-time fluorescent one-step PCR in the differential detection of H5, H9, H7 subtype avian influenza inactivated vaccines. The result showed that the method was specific and reproducible. No cross-reaction was discovered with other avian disease vaccines. Real-time fluorescent PCR provided a specific, sensitive, rapid and convenient method for the subtype identification of avian influenza inactivated vaccines.
Animals
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Hemagglutinin Glycoproteins, Influenza Virus
;
immunology
;
Humans
;
Influenza A Virus, H5N1 Subtype
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immunology
;
Influenza A Virus, H7N7 Subtype
;
immunology
;
Influenza A Virus, H9N2 Subtype
;
immunology
;
Influenza A virus
;
classification
;
immunology
;
Influenza Vaccines
;
analysis
;
classification
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Reverse Transcriptase Polymerase Chain Reaction
;
methods
;
Vaccines, Inactivated
;
analysis
3.Progress in new vaccine strategies against influenza: a review.
Zhihui LIU ; Tao JIANG ; Ede QIN ; Duoliang RAN ; Chengfeng QIN
Chinese Journal of Biotechnology 2012;28(5):550-556
Influenza, caused by influenza virus, is a serious respiratory illness which poses a global public health threat. Vaccination is the primary strategy for the prevention and control of influenza. Although both inactivated vaccines and the live attenuated vaccines are effective in preventing influenza, the current vaccines have poor efficacy in the elderly and fail to provide protection against heterosubtype viruses. Development of a safer and more effective influenza vaccine that provides broad cross protection, overcoming the intrinsic limitation of the current vaccines, has been a scientific challenge. During the past decades, structural biology, reverse genetic and other virological technologies developed quickly and sped the progress of influenza vaccinology. Some new strategies for developing influenza vaccine have been generated, produced encouraging results, which showed great prospect as next-generation of influenza vaccines.
Disease Outbreaks
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prevention & control
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Hemagglutinin Glycoproteins, Influenza Virus
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immunology
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Humans
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Influenza Vaccines
;
biosynthesis
;
immunology
;
Influenza, Human
;
immunology
;
prevention & control
;
virology
;
Orthomyxoviridae
;
immunology
;
Vaccines, Attenuated
;
immunology
;
Vaccines, Inactivated
;
immunology
4.Hemagglutinin stem reactive antibody response in individuals immunized with a seasonal influenza trivalent vaccine.
Xiaopeng ZHAO ; Kun QIN ; Jinlei GUO ; Donghong WANG ; Zi LI ; Wenfei ZHU ; Liqi LIU ; Dayan WANG ; Yuelong SHU ; Jianfang ZHOU
Protein & Cell 2015;6(6):453-457
Adult
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Antibodies, Viral
;
blood
;
immunology
;
Cross Reactions
;
Hemagglutinin Glycoproteins, Influenza Virus
;
immunology
;
Humans
;
Influenza A Virus, H1N1 Subtype
;
immunology
;
Influenza A Virus, H3N2 Subtype
;
immunology
;
Influenza B virus
;
immunology
;
Influenza Vaccines
;
immunology
;
Orthomyxoviridae
;
immunology
;
Seasons
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Vaccination
5.Identification of Epitopes for Neutralizing Antibodies Against H10N8 Avian Influenza Virus Hemagglutinin.
Jin-Fang HU ; Chun-Yun SUN ; Mu-Ding RAO ; Liang-Zhi XIE
Acta Academiae Medicinae Sinicae 2016;38(4):404-410
Objective To develop neutralizing monoclonal antibodies (MAbs) against H10N8 avian influenza virus hemagglutinin and to identify the binding sites. Methods MAbs against hemagglutinin of H10N8 avian influenza virus were developed by genetic engineering. Neutralizing MAbs were screened by microneutralization assay,and then tested by enzyme-linked immunosorbent assay and Western blot to identity the binding sites.The homology modeling process was performed using Discovery Studio 3.5 software,while the binding epitopes were analyzed by BioEdit software. Results One MAb that could neutralize the H10N8 pseudovirus was obtained and characterized. Analysis about epitopes suggested that the antibody could bind to the HA1 region of hemagglutinin,while the epitopes on antigen were conserved in H10 subtypes.Conclusions One neutralizing antibody was obtained by this research.The MAb may potentially be further developed as a pre-clinical candidate to treat avian influenza H10N8 virus infection.
Antibodies, Monoclonal
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immunology
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Antibodies, Neutralizing
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immunology
;
Antibodies, Viral
;
immunology
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Enzyme-Linked Immunosorbent Assay
;
Epitopes
;
immunology
;
Hemagglutinin Glycoproteins, Influenza Virus
;
immunology
;
Influenza A Virus, H10N8 Subtype
;
Neutralization Tests
6.Advances in the structure and function of pandemic A/H1N1/2009 influenza virus HA protein.
Wen-Qiang ZHANG ; Shao-Xia SONG ; Tong-Zhan WANG
Chinese Journal of Virology 2012;28(4):444-452
Since March 2009, pandemic A/H1N1/2009 influenza virus has been spreading throughout many countries including China. The emerged virus caused great harm to human health and social economy. Hemagglutinin (HA) is the most important viral surface glycoprotein, mainly possessing three kinds of functions: (1) binding to host cell receptor, (2) triggering the fusion between viral envelop and target cell membrane, (3) stimulating the body to generate the neutralizing antibody. Advances in the structure, primary function, evolution and antigenicity of pandemic A/H1N1/2009 influenza virus HA protein are reviewed in this paper.
Animals
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Evolution, Molecular
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Hemagglutinin Glycoproteins, Influenza Virus
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chemistry
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genetics
;
immunology
;
metabolism
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Humans
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Influenza A Virus, H1N1 Subtype
;
genetics
;
immunology
;
pathogenicity
;
physiology
;
Influenza, Human
;
epidemiology
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virology
;
Pandemics
8.Antigenic and genetic study of influenza virus circulated in China in 2006.
Ye ZHANG ; Xiang ZHAO ; Jun-feng GUO ; He-jiang WEI ; Yan-hui CHENG ; Xin-wan LI ; Cui-lin XU ; Yuan-ji GUO ; Yue-long SHU
Chinese Journal of Experimental and Clinical Virology 2007;21(4):304-306
OBJECTIVETo analyse seasonal influenza epidemic situation in 2006, and to analyse the genetic and antigenic characteristics of viral hemagglutinin (HA) gene.
METHODSThe single-way hemagglutination inhibition (HI) tests were used to test the antigenic characteristics of these viruses from influenza surveillance network, and the HA1 genes were sequenced based on the antigenic test results according to different isolation times and sites.
RESULTSThe influenza virus types A and B co-circulated in 2006. influenza A H1N1 subtype and Victoria-like B influenza circulated preponderantly during this epidemic season. The HA1 gene sequence of H1N1 viruses showed that 192, 193, 196, 198 positions (located at antigenic site B) have an amino acid substitute, compared with the last circulating strain A/Hubeihongshan/53/2005(H1N1). Two amino acid changes at 142 and 144 positions compared with A/Yunnan/1145/2005 (H3N2). There was no change in influenza B viruses either Victoria-like B or Yamagata-like B virus, i.e . antigenic characteristics is analogous to B/shenzhen/155/2005 and B/tianjin/144/2005, respectively.
CONCLUSIONThe H1N1 and H3N2 influenza viruses had changing antigenic and genetic characteristics in 2006. Influenza virus types B did not change in 2006.
Amino Acids ; analysis ; China ; Hemagglutination Inhibition Tests ; Hemagglutinin Glycoproteins, Influenza Virus ; chemistry ; genetics ; immunology ; Influenza A Virus, H1N1 Subtype ; immunology ; isolation & purification ; Influenza A Virus, H3N2 Subtype ; immunology ; isolation & purification ; Influenza B virus ; immunology ; isolation & purification ; Time Factors
9.Crystal structure of the swine-origin A (H1N1)-2009 influenza A virus hemagglutinin (HA) reveals similar antigenicity to that of the 1918 pandemic virus.
Wei ZHANG ; Jianxun QI ; Yi SHI ; Qing LI ; Feng GAO ; Yeping SUN ; Xishan LU ; Qiong LU ; Christopher J VAVRICKA ; Di LIU ; Jinghua YAN ; George F GAO
Protein & Cell 2010;1(5):459-467
Influenza virus is the causative agent of the seasonal and occasional pandemic flu. The current H1N1 influenza pandemic, announced by the WHO in June 2009, is highly contagious and responsible for global economic losses and fatalities. Although the H1N1 gene segments have three origins in terms of host species, the virus has been named swine-origin influenza virus (S-OIV) due to a predominant swine origin. 2009 S-OIV has been shown to highly resemble the 1918 pandemic virus in many aspects. Hemagglutinin is responsible for the host range and receptor binding of the virus and is therefore a primary indicator for the potential of infection. Primary sequence analysis of the 2009 S-OIV hemagglutinin (HA) reveals its closest relationship to that of the 1918 pandemic influenza virus, however, analysis at the structural level is necessary to critically assess the functional significance. In this report, we report the crystal structure of soluble hemagglutinin H1 (09H1) at 2.9 Å, illustrating that the 09H1 is very similar to the 1918 pandemic HA (18H1) in overall structure and the structural modules, including the five defined antiboby (Ab)-binding epitopes. Our results provide an explanation as to why sera from the survivors of the 1918 pandemics can neutralize the 2009 S-OIV, and people born around the 1918 are resistant to the current pandemic, yet younger generations are more susceptible to the 2009 pandemic.
Animals
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Cloning, Molecular
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Crystallography, X-Ray
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Hemagglutinin Glycoproteins, Influenza Virus
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chemistry
;
genetics
;
immunology
;
isolation & purification
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Influenza A Virus, H1N1 Subtype
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chemistry
;
genetics
;
immunology
;
Models, Molecular
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Protein Conformation
;
Swine
;
virology
10.Evaluation of the immune response to recombinant DNA vaccine and adenoviral vaccine co-expressing the M1 and HA genes of H5N1 influenza virus in mice.
Jianqiang GUO ; Lihong YAO ; Aijun CHEN ; Xiaoyu LIU ; Jinqi FU ; Pengwei XU ; Zhiqing ZHANG
Chinese Journal of Biotechnology 2011;27(6):876-883
In order to evaluate the response to vector-expressed M1 and HA genes of influenza virus in mice, we prepared recombinant plasmid pStar-M1/HA and recombinant adenovirus Ad-M1/HA containing both the full-length matrix protein 1(M1) and hemagglutinin (HA) genes of human H5N1 influenza virus strain A/Anhui/1/2005. We then combined the DNA vaccine and adenoviral vaccine in immunization of BALB/c mice with a prime-boost regime. We immunized the mice with DNA vaccine at day 0 and 28 and with recombinant adenoviral vaccines at day 14 and 42. We took blood samples before each injection and 14 days after the final injection for detection of humoral immune responses. At day 56, we sacrificed the mice and collected splenocytes for detection of cellular immune responses. ELISA and hemagglutination inhibition (HI) assay showed that specific IgG Abs against H5N1 influenza virus was induced in serum of the immunized mice. ELISPOT results confirmed that the specific cellular immune responses were successfully induced against the M1 and HA proteins of H5N1 influenza virus. This study provides new strategy for development of novel influenza vaccines.
Adenoviridae
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genetics
;
metabolism
;
Animals
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Antibodies, Viral
;
blood
;
Hemagglutinin Glycoproteins, Influenza Virus
;
genetics
;
immunology
;
Immunization
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Influenza A Virus, H5N1 Subtype
;
immunology
;
Influenza Vaccines
;
immunology
;
Mice
;
Mice, Inbred BALB C
;
Recombinant Fusion Proteins
;
genetics
;
immunology
;
Vaccines, DNA
;
immunology
;
Viral Matrix Proteins
;
genetics
;
immunology