2.Identification of Br platelet antigens using modified mixed passive hemagglutination.
Nam Kyung KIM ; Jang Soo SUH ; Jay Sik KIM ; Dal Hyo SONG ; Young Chul OH ; Ki Hong KIM
Korean Journal of Blood Transfusion 1992;3(2):151-157
No abstract available.
Blood Platelets*
;
Hemagglutination*
4.A Study on the Validity of Passive Hemagglutination (PHA) Test for HBsAb.
Korean Journal of Preventive Medicine 1987;20(1):114-119
The author investigated the effect of some variables such as age, sex and the experience of past vaccination on the validity of PHA. The changing pattern of the validity with the change of PHA diagnostic criteria, and the relationship between PHA test result and RIA Ratio Unit were also studied. The results obtained were as follows; 1) No statistically significant difference was found in sensitivity, specificity and negative predictability by sex, but positive predictability was significantly higher in male than that in female. 2) Positive predictability was shown to become higher with the increase of age and negative predictability was found to be significantly different among age groups, but no statistically significant difference was found in sensitivity and specificity by age group. 3) Significantly low specificity and high positive predictability were found in past vaccined group, but no statistically significant difference was found in sensitivity and negative predictability between past vaccined group and non-vaccined group. 4) False negative cases by PHA were found to be the weak positive reactors by RIA and false positive rate of PHA was as high as 46.3 percent. 5) Sensitivity and specificity of PHA at the diagnostic criteria of HBsAb titer 1 : 2 were 98.4% and 53.8% respectively, but after increasing the HBsAb titer to 1 : 64 as the diagnostic criteria the results were 60.0% and 95.6% respectively.
Female
;
Hemagglutination*
;
Humans
;
Male
;
Sensitivity and Specificity
;
Vaccination
5.Two AB3 and a B3 Cases from a Family.
Byung Kee KANG ; Won Kyu KIM ; Woo In LEE ; Hee Joo LEE
Korean Journal of Clinical Pathology 1997;17(5):853-857
We experienced two AB3 and a B3 from a 27-year-old student and his family. B3 subgroup was confirmed by delayed and weak mixed-field agglutination with anti-B serum, adsorption-elution test, serum and saliva hemagglutination inhibition test and family study. We report a family case of AB3 and B3 with brief review of literatures.
Adult
;
Agglutination
;
Hemagglutination Inhibition Tests
;
Humans
;
Saliva
6.Two AB3 and a B3 Cases from a Family.
Byung Kee KANG ; Won Kyu KIM ; Woo In LEE ; Hee Joo LEE
Korean Journal of Clinical Pathology 1997;17(5):853-857
We experienced two AB3 and a B3 from a 27-year-old student and his family. B3 subgroup was confirmed by delayed and weak mixed-field agglutination with anti-B serum, adsorption-elution test, serum and saliva hemagglutination inhibition test and family study. We report a family case of AB3 and B3 with brief review of literatures.
Adult
;
Agglutination
;
Hemagglutination Inhibition Tests
;
Humans
;
Saliva
7.3 Cases of Ocular Toxoplasmosis.
Sang Mook KONG ; Tae Soo LEE ; Chung Whan KIM
Journal of the Korean Ophthalmological Society 1975;16(2):141-145
The authors have recently experienced 3 cases of ocular toxoplasmosis. The diagnosis was based on typical ocular lesions and hemagglutination test for toxoplasmosis. In addition to some clinical observations, a brief review of literature has been described.
Diagnosis
;
Hemagglutination Tests
;
Toxoplasmosis
;
Toxoplasmosis, Ocular*
8.Factors Affecting Hemagglutinations Strength in ABO Blood Group Typing Test Using the Tube Method.
You La JEON ; Woo In LEE ; So Young KANG ; Myeong Hee KIM
Journal of Laboratory Medicine and Quality Assurance 2018;40(3):161-170
BACKGROUND: The ABO blood group typing test (ABO test) is an initial pre-transfusion test based on hemagglutination. Although various factors affect hemagglutination strength, few studies have examined how these factors can be applied in clinical laboratories and their effects on hemagglutination. This study was conducted to analyze the factors affecting hemagglutination strength in the ABO test using a tube method applied in many laboratories. METHODS: We conducted a detailed questionnaire survey of 51 laboratories which use the ABO test with a tube method. We also analyzed the results of the ABO test (cell and serum typing) with 40 specimens using factors affecting hemagglutination at a tube method and applied differently in each laboratory. RESULTS: Each laboratory used various methods to prepare red cell suspensions as specimens or reagents and used different reagent to sample ratios, centrifugation protocols, and shaking test tubes before evaluating hemagglutination strength. By testing various combinations of these factors, direct sampling from the red cell layer of the original specimen was found to have the largest effect on lowering hemagglutination strength in cell typing tests. In serum typing tests, various factors influenced hemagglutination strength, including shaking the tube before analysis and the concentration of a home-made red cell suspension used as a reagent. CONCLUSIONS: To achieve accurate results in the ABO test by the tube method, detailed guidelines that include the factors affecting hemagglutination strength determined in this study should be established.
Centrifugation
;
Hemagglutination*
;
Indicators and Reagents
;
Methods*
;
Suspensions
9.Evaluation of immunogenicity of the 2008-2009 seasonal influenza vaccines by microneutralization test.
Seung Youn KIM ; Yun Kyung KIM ; Byung Wook EUN ; Nam Hee KIM ; Eun Kyeong KANG ; Byong Sop LEE ; Jung Sub LIM ; Jun Ah LEE ; Dong Ho KIM
Korean Journal of Pediatrics 2012;55(12):474-480
PURPOSE: For evaluating the immunogenicity of an influenza vaccine, the microneutralization (MN) test has a higher sensitivity and specificity as compared to the hemagglutination inhibition (HI) test. However, the MN test is more time consuming and is difficult to standardize. We performed the MN test to determine its usefulness as an alternative or complementary test to the HI test for evaluating the immunogenicity of influenza vaccines. METHODS: We compared the MN test with the HI test using 50 paired samples taken from a previous clinical study (2008-2009) in Korean children under 18 years of age. RESULTS: The linear correlation coefficients of the 2 tests for H3N2, H1N1, and influenza B were 0.69, 0.70, and 0.66, respectively. We identified a high index of coincidence between the 2 tests. For an influenza vaccine, the postvaccination seroprotection rates and seroconversion rates determined by the MN test were 78.0% and 96.0%, 90% and 42.0%, and 42.0% and 48.0% for H3N2, H1N1, and influenza B, respectively. Geometric mean titer fold increases of H3N2, H1N1, and influenza B were 2.89, 5.04, and 4.29, respectively, and were 2.5-fold higher. We obtained good results in the evaluation of the immunogenicity of the 2008-2009 seasonal influenza vaccines. CONCLUSION: We found that the MN test was as effective as the HI test. Therefore, we suggest that the MN test can be used as an alternative or complementary test to the HI test for evaluating the immunogenicity of influenza vaccines.
Child
;
Hemagglutination
;
Hemagglutination Inhibition Tests
;
Humans
;
Influenza Vaccines
;
Influenza, Human
;
Neutralization Tests
;
Seasons
;
Sensitivity and Specificity
10.Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency.
Mi Ja PARK ; Seong Joon JOH ; Kang Seuk CHOI ; Aeran KIM ; Min Goo SEO ; Jae Young SONG ; Seon Jong YUN
Korean Journal of Veterinary Research 2016;56(3):189-192
The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The r² values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen's kappa coefficient between the VN titer of 2 log₁₀ and HI titer of 5 log₂ was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.
Bronchitis
;
Enzyme-Linked Immunosorbent Assay*
;
Hemagglutination Inhibition Tests*
;
Hemagglutination*
;
Infectious bronchitis virus*
;
Neutralization Tests*
;
Vaccine Potency*