1.Best Operation Mode of AP-960 Full-automated ELISA System
Huijun ZHU ; Zhikang XU ; Helu LIU ; Xiaoxing YANG ; Zhenping LIU
Chinese Medical Equipment Journal 1993;0(05):-
Objective To compare the operation modes of AP-960 full automated ELISA system.Methods Two models were tested: beginning with adding specimens(model 1) and beginning with adding enzymes(model 2).The following factors were compared: the consumption of suckers,usage of time,result of test and the usual alarm during the test.Results The consumptions of suckers were 564 and 84 respectively.The needing time of the former mode was 125 minutes and that of the latter mode was 90 minutes.Conclusion The mode beginning with adding enzymes(model 2) is better for the current status of our clinical laboratory.
2.Development and application of network information system for laboratory equipment management
Helu LIU ; Ningzheng LUO ; Zhikang XU ; Zhenping LIU ; Yulin GAO
Chinese Medical Equipment Journal 1989;0(01):-
Objective To develop a set of network information system for laboratory equipment management. Methods According to the requirement of laboratory equipment management provided by laboratory section, a professional laboratory software company developed the software by using SQL Server 2000 as database and Delphi 7.0 as program language. Results Data of laboratory equipment in hospital were shared and communicated. Laboratory equipment management was standardized and work efficiency and quality were improved. Conclusion The software is convenient and practical with high popularization and application value.
3.Diagnostic and predictive value of troponin I hy persensitive C-reactive protein and lactic acid in viral myocarditis
Zhenwen YANG ; Helu LIU ; Yong JIANG ; Xiaohua PAN ; Cheng YANG ; Ping LIU
Chinese Journal of Primary Medicine and Pharmacy 2012;19(1):5-6
ObjectiveTo explore the diagnostic and predictive value of troponin Ⅰ,lactic acid and hypersensitive C-reactive protein(hs-CRP) in viral myocarditis.MethodsTroponin Ⅰ,hs-CRP,lactic acid blood levels in different time were measured in 88 patients with acute viral myocarditis(54 cases) or acute upper respiratory infections (34 cases).Troponin Ⅰ leyel in patients with severe 39 cases and mild VMC 15 cases were compared.ResultsTroponin Ⅰ level of VMC group was (0.59 ±0.10) ng/L,and significantly higher than that of acute upper respiratory infection group [ (0.10 ± 0.08 ) ng/L ] ( t =2.79,P < 0.05 ).And higher lactic acid and hs-CRP level were observed at different period ( t =2.71,2.48,all P < 0.05).ConclusionTroponin I could help diagnose VMC predict the severity of myocarditis to some extent.Lactic acid and hs-CRP could also reflect imflammation injury of VMC.
4.Establishment and Application of TaqMan Real-Time PCR assay for Diagnosis of Streptococcus Pneumoniae
Dongyue LU ; Helu LIU ; Yue HE ; Peipei LI ; Hong LIANG ; Shue ZHU
Journal of Modern Laboratory Medicine 2014;(5):60-63
Objective To develop TaqMan real-time PCR assay for detection and identification of streptococcus pneumonia iso-lated from children CAP.Methods Based on the sequences of lytA gene,primers and probe were designed and the assay was optimized.Then 1 504 sputum samples were detected by culture and the developed assay with double-blind testing.Results The lower limit of detection in the developed real-time PCR assay was 18.75 cfu/PCR,and had no cross reaction.141 strep-tococcus pneumonia strains were detected from 1 504 samples and 140 were isolated by culture.The whole process just nee-ded 2.5 h.Conclusion The established assay is rapid,simple,high sensitivity and specificity.It is not only valuable for the i-dentification of streptococcus pneumonia,but also provide evidence for antibiotic therapy.
5.Establishment and Application of SYBR Green I Real-Time PCR Assay for Rapid Detection of Hepatitis B Virus DNA
Weina HE ; Dongyue L¨U ; Helu LIU ; Jiehui HAN ; Yue HE ; Peipei LI
Journal of Modern Laboratory Medicine 2016;31(3):98-101
Objective To develop SYBR Green I real-time PCR assay for detection and identification of Hepatitis B virus. Methods Based on the sequences of Hepatitis B virus gp1 gene,primers were designed.The reaction assay and thermal cyc-ling profile were optimized.The positive standard was from recombinant clone.Both the developed assay and Zhejiang kuake biotechnology company’s assay were applied in 100 patients serum.Results The detection limit was between 5×102 copies/ml to 5×108 copies/ml with a good liner correlation and no cross reaction.The whole process just needed 2.5 h.Comparing with the company products,the sensitivity and specificity of the developed assay were 100% and 92.5% respectively.Con-clusion The established assay is rapid,simple,high sensitivity and specificity.It is not only valuable for the identification of Hepatitis B virus patients,but also provide accurate quantitative analysis for HBV patients.