OBJECTIVETo study the chemical constituents of Helichrysi flos syn.

METHODThe chemical constituents were isolated by various methods of isolation (silica gel, ODS column chromatography and HPLC) and their structure were elucidated by the analysis of spectral data and chemical properties.

RESULTSeven flavone compounds were isolated from the MeOH extract of Helichrysi flos syn. The chemical structures were elucidated as quercetin-3-O-beta-D-glucopyranoside (1), apigenin-7-O-beta-D-gentiobioside (2), quercetin-3-O-rutinoside (3), apigenin-7,4'-di-O-beta-D-glucopyranoside (4), kaempferol-3,4'-di-O-beta-D-glucopyranoside (5), quercetin-3,3'-di-O-beta-D-glucopyranoside (6), apigenin-7-O-beta-D-glucopyranosiduronic acid methyl ester (7).

CONCLUSIONCompounds 2, 4-7 were obtained from the genus Helichrysum for the first time.

Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; analysis ; Flavones ; analysis ; Helichrysum ; chemistry

AIM: The aqueous methanolic extracts of two plants from Algeria, Helichrysum stoechas subsp. rupestre and Phagnalon saxatile subsp. saxatile, were investigated for their antioxidant activity. METHOD: Total phenolics, flavonoids, and tannins were determined by spectrophotometric techniques. In vitro antioxidant and radical scavenging profiling was determined by spectrophotometric methods, through: Total antioxidant capacity, and radical scavenging effects by the DPPH and ABTS methods, reducing and chelating power, and blanching inhibition of the β-carotene. RESULTS: All of the extracts showed interesting antioxidant and radical scavenging activity. The highest contents in phenolics, tannins, and the highest total antioxidant capacity as gallic acid equivalents of 97.5 ± 0.33 mg GAE/g DW was obtained for the flowers of H. stoechas subsp. rupestre extract in the phosphomolybdenum assay. An extract of the leafy stems of P. saxatile subsp. saxatile revealed the highest content of flavonoids, and the highest antioxidant activity by the radical scavenging and β-carotene assays when compared with standards. The best activity was by the scavenging radical DPPH with an IC50 value of 5.65 ± 0.10 μg·mL(-1). CONCLUSION The studied medicinal plants could provide scientific evidence for some traditional uses in the treatment of diseases related to the production of reactive oxygen species (ROS) and oxidative stress.
Algeria ; Antioxidants ; analysis ; pharmacology ; Asteraceae ; chemistry ; Benzothiazoles ; metabolism ; Biphenyl Compounds ; metabolism ; Flavonoids ; analysis ; pharmacology ; Helichrysum ; chemistry ; Oxidative Stress ; drug effects ; Phenols ; analysis ; pharmacology ; Picrates ; metabolism ; Plant Extracts ; chemistry ; pharmacology ; Plant Structures ; chemistry ; Spectrophotometry ; methods ; Sulfonic Acids ; metabolism ; Tannins ; analysis ; pharmacology