Objective To study the surgical techniques and clinical experience of the total laparoscopic splenectomy .Methods Retrospective analysis 31 cases were performed laparoscopic splenectomy from January 2003 to April 2015 .Among them ,there were 12 cases with liver cirrhosis and hypersplenism ,5 cases with spleen hemangiomatosis ,3 cases with splenic lymphoma ,3 cases with splenic cyst ,2 cases with obsolete rupture of spleen hematoma ,6 cases with idiopathic thrombocytopenia .During surgery ,Li‐gaSure was used to deal with stomach ligament splenic artery .Using silk or endoscopic linear cutters to process splenic pedicle indi‐vidually .Results All operations were performed successfully without conversion to open surgery .13 patients were cutting off splenic pedicle by ligating of splenic portal blood vessel and 18 patients were using endoscopic linear cutters .The operation time was 80-240 min ,mean (130 ± 35)min ,the blood loss was 60-500 mL ,mean (150 ± 80)mL ,hospital stay was 8 .3 days .There was no mortality and pancreatic leakage complications .Conclusion On the basis of open surgery ,evaluating carefully before operation ,se‐lecting appropriate cases ,using different laparoscopic instruments ,handling splenic pedicle individually ,can make the laparoscopic splenectomy be a more safe and useful operation modality .

Objective To study the effects of Yizhichan moxibustion on chronic fatigue syndrome (CFS) athletes.Methods 143 patients of CFS were blind randomly devided into3 groups, which were moxibustion of Yizhichan (56 cases) group, general moxibustion group (42 cases), and normal group (45 cases).The normal group was treated by passive massage for relaxation, the moxibustion of Yizhichan group was treated by specific acupoints application of moxibustion of Yizhichan in addition to passive massage;and the general moxibustion groupwas treated by general moxibustion in addition to passive massage.All sample were treated once a day for 6 days in a week.3 weeks was a period of treatment, and the total time of treatment was 15 weeks.Results After treatment, the sleep quality (1.73 ± 0.73, 1.54 ± 0.85 vs.0.83 ± 0.52, F=14.130), time for sleep (1.57 ± 0.60, 1.35 ± 0.60 vs.1.03 ± 0.54, F=5.386), sleep time (1.78 ± 0.71, 1.77 ± 0.61 vs.0.67 ± 0.65, F=19.811), daytime dysfunction (0.78 ± 0.83, 1.64 ± 0.83 vs.1.03 ± 0.83, F=4.798) in the control group, moxibustion of Yizhichan group and a general moxibustion group were statistically significant(P＜0.05).The total effective rate of the moxibustion of Yizhichan group was better than the control group and the general moxibustion group (83.8％, 42.2％ vs.63.6％;x2=13.973, P=0.001).Conclusion Specific acupoints moxibustion of Yizhichan is a beneficial exploration of moxibustion methods of traditional Chinese medicine, and can improve CFS athletes level of physical health, attention and sleep quality.

Objective To observe effects of artemisia argyi moxibustion therapy Yizhichan (one finger Zen) on sleep, attention ability and TCM constitution of athletes suffering chronic fatigue syndrome (CFS);To study the safety intervention model of CFS athletes.Methods Fifty-three cases of CFS athletes were divided into control group of 26 cases and trial group of 27 cases through random single-blind method. The control group was treated with passive gimmick massage relaxation process on waist and limbs. Based on this basic treatment, the trial group was treated with application of artemisia argyi moxibustion therapy Yizhichan. After 3 treatment course, the changes of SCL-90 scale, letter cancellation test, Pittsburgh sleep quality index scale (PSQI), and TCM constitution were observed.Results After treatment, SCL-90 scale, other two factor scores, time of letter cancellation test, PSQI sleep quality, falling sleep time, sleep time, sleep disorder, daytime dysfunction project factor scores of PSQI, and the peaceful constitution had statistical significance between the two groups (P<0.05).Conclusion Specific artemisia argyi moxibustion therapy Yizhichan and fumigation can safely and effectively treat athletes with CFS on physical health, attention level and sleep quality, which is beneficial for the exploration of traditional moxibustion methods.

Objective To investigate influence of Qi-strengthening,blood-activating and toxin-removing therapy on plasma and colonic P-selectin in ulcerative colitis(UC) rats.Methods Trinitrobenzene sulfonic acid(TNBS) was used for the establishment of SD rat models of UC.UC rats were randomized into the model group,western medicine group(gastric gavage of suspension of Olsalazine Sodium Capsules 0.266 g?kg-1?d-1),and herbal medicine group(gastric gavage of Kuijie Fufa Recipe 20 g?kg-1?d-1).Meanwhile,a normal control group was set up.After medication for 10 and 30 days,and after drug withdrawal for 10 days,plasma P-selectin level was detected by enzyme-linked immunosorbent assay,and colonic P-selectin expression was detected by immunohistochemical assay.Results Plasma P-selectin level was increased in the model group in different time(P

Objective: To understand the level of health literacy and influencing factors of Chinese general high school students, so as to provide scientific evidence for health promotion of high school students. Methods: A multistage stratified cluster sampling method was used to conduct an on site survey on the health literacy status of 8 265 high school students in 31 provinces of China by using the National Health Literacy Monitoring Questionnaire from October 2018 to June 2019. SPSS 25.0 software was used to conduct one way test and multifactor Logistic regression analysis of different demographic characteristics. Results: The health literacy level of Chinese general high school students was 7.1%; the three dimensions of literacy were ranked in descending order as follows: basic skills (21.3%), healthy lifestyles and behaviors (20.5%), and basic knowledge and concepts of health (7.8%); and the literacy levels of the six categories of health issues, in descending order, were basic medical care (88.3%), health information (75.9%), safety and first aid (51.0%), infectious disease prevention (44.2%), scientific health concept (39.0%) and chronic disease prevention (5.5%). Multifactorial Logistic regression analysis showed that ethnicity, region, family residence, school type, grade level, and father s education were the factors influencing the health literacy level of Chinese high school students( OR=2.08, 0.60/0.24, 0.44, 0.71, 1.41 /0.51, 1.37, P <0.05). Conclusions The overall health literacy level of Chinese high school students is low，ethnicity, region, family residence, school type, grade level and father s education are factors influencing the health literacy level of Chinese high school students. It is recommended that all departments at all levels focus on categorized governance to improve the health literacy level of high school students, taking into account the realities and characteristics of high school students health literacy level.

Objective:To investigate the current situation of sitting time and health literacy among high school students in China,in order to provide a basis for improving their physical and mental health levels.Methods:A stratified random cluster sampling method was used to investigate the length of sitting time and health literacy of first and second grade high school students from 31 provinces,cities,and au-tonomous regions in China(data did not include that of Hong Kong and Macao Special Administrative Re-gion,and Taiwan Province of China).The Kruskal-Wallis H method,independent sample Mann-Whit-ney U test,and regression model were used to analyze the influencing factors of sitting time and total health literacy score.Results:(1)The total score of health literacy was statistically significant(P＜0.01)in different regions,urban and rural distribution,annual family income,parents'educational background,age,and gender.(2)The length of sitting was statistically significant(P＜0.01)among multiple groups in different regions,family annual income,parental education,and gender.However,there was no statistically significant difference between groups of different ages and urban-rural distribution(P＞0.05).(3)The analysis of multiple linear regression model showed that the total score of health literacy was positively correlated with the family's annual income and the mother's education,and nega-tively correlated with the father's education and the length of sitting.Standardized regression coefficientβcomparison:Father's education(-0.32)＞family annual income(0.15)＞mother's education(0.09)＞average daily sitting time(-0.02),with father's education having the greatest impact,fol-lowed by family annual income.The length of sitting was positively related to the family's annual income and the mother's educational background,and negatively related to the total score of health literacy.Standardized regression coefficientβ comparison:Annual family income(0.14)＞education background of mother(0.13)＞total score of health literacy(-0.02),with the impact of annual family income the largest,followed by education background of mother.Conclusion:China's first and second grade high school students generally spend a long time sitting every day,and the level of health literacy is generally low.The level of health literacy and sitting time are negatively correlated with each other,and are most in-fluenced by the educational background of high school students'parents and their family economic levels.

Objective: To investigate the expression of Apelin and Snail proteins in breast cancer and their relationship with the clinicopathological features and prognosis. Methods: The expression of Apelin and Snail proteins was detected by immunohistochemistry in 89 cases of breast cancer and 50 cases of mammary adenosis collected from January to June in 2008 at Fujian Cancer Hospital; the expression was correlated with the clinicopathological features and outcome of the patients. Results: Apelin and Snail were expressed in 42 cases(47.2%)and 36 cases(40.4%)of breast cancers, respectively, and the expression was higher than that of control group (P<0.01). The expression of Apelin was positively correlated with Snail (r=0.230, P<0.05). Apelin expression was associated with lymph node metastasis and TNM staging(P<0.05). Snail expression was associated with lymph node metastasis(P<0.05). Kaplan-Meier survival curve showed that the prognosis of Apelin positive group was worse than that of Apelin negative group (P<0.01). There was no significant difference in prognosis between Snail negative and positive groups (P>0.05). The prognosis of Apelin and Snail in both positive groups was worse than that of Apelin and Snail both negative groups (P<0.01). Multivariate COX regression analysis showed that Apelin and TNM staging could be used as independent prognostic factors for breast cancer (P<0.05). Conclusions Apelin and Snail are highly expressed in breast cancer, and associated with lymph node metastasis and TNM stage. There is a positive correlation between Apelin and Snail expression, which may suggest a role in breast carcinogenesis. The prognosis of breast cancer with expression of Apelin and co-expression of Apelin and Snail is poor. Therefore, Apelin may be used as an effective indicator to evaluate the prognosis of breast cancer patients.

Objective: To investigate the clinicopathologic features of microcystic, elongated and fragmented (MELF) pattern invasion of endometrial adenocarcinoma. Methods: HE and immunohistochemistry staining method were used to analysis morphologic features and immunophenotype of 72 patients of endometrial adenocarcinoma with MELF pattern invasion, and chi-square test was used to analysis the clinicopathologic features. Results: The mean age of 72 patients was 54 years (40 to 70 years). Thirty-two patients were pre-menopausal and 40 were post-menopausal. According to the FIGO staging system (2014), 32 cases(44.4%)were at stage Ⅰ, 22 cases(30.6%)at stage Ⅱ, 17 cases(23.6%)at stage Ⅲ and 1 case(1.4%) at stage Ⅳ. Microscopically, MELF invasion showed microcystic, elongated slit-like or fragmented glands in myometrium and their lining cells usually were cube or flat, as well as the single or clusters of eosinophilic tumor cells mimicking histocytes. In addition, a fibromyxoid or inflammatory stromal response was often present.Immunohistochemical staining showed that MELF invasion was positive for p16, CA125 and CA19-9, but negative for ER, PR and p53.Compared with non-MELF pattern invasion, significant differences were noted in menopause pausimenia, FIGO stages, deep invasion into myometrium, lymph metastasis, lymphovascular space invasion (LVSL), serum CA125 and CA19-9 in patients with MELF pattern invasion (all P<0.05). Conclusions MELF pattern invasion of endometrial adenocarcinoma is characterized by advanced FIGO stage, deep myoinvasion, high metastasis rate to lymph node and LVSL. Pathologists should recognize the MELF invasion and evaluate the depth of myometrium of infiltration and LVSL with special attention to the presence of MELF invasion with necessary immunohistochemistry for more accurate pathological diagnosis.