24 dogs for experiments were divided into 3 groups: total splenectomy, sham-operation and splenic autologous transplantation (SAT). Results of the study showed that in dogs the SAT (each splenic piece size 110.5 cm) into the omental pouch did survive well,and restore a part of function. The function of neutro-phil chemoluminescence and the phagocytosis rate of neutrophils, after splenectomy, in vitro, were lower than that of sham-operation group. The dogs of SAT could give some protective action, but the number of T-lymphocytes in splenic corpuscles and mononuclear phagocytes in splenic red pulps in regenerated splenic tissue were lower that of normal splenic tissue.