1.The Effectiveness of Tocotrienol Rich Fraction and Αlpha Tocoferol with Combination of Vitamin C in The Management of Systemic Inflammatory Response Syndrome (SIRS)
Husam YE ; Raha AR ; Jaafar MZ ; Mohd Heikal MY
Journal of Surgical Academia 2017;7(2):4-12
The pathophysiology of systemic inflammatory response syndrome (SIRS) had been described to involve various
strong oxidative reactions affecting the status and progress of the patients. Antioxidant therapy had been suggested in
many studies involving SIRS management. The objective of this study was to compare the role of vitamin E
Tocotrienol and vitamin E Tocopherol combined with vitamin C as antioxidant therapy in the management of
critically ill patients diagnosed with SIRS, admitted to the intensive care unit and high dependency wards of
Universiti Kebangsaan Malaysia Medical Centre (UKMMC). It was a single blind randomized clinical trial with a
total of 72 patients in which 44.4% Malays, 34.7% Chinese, 19.4% Indians and 1.4% others with 59.7% males and
40.3% females were recruited. Patients in TRI E group received Tocotrienol with Vitamin C while TOCO group
received Tocopherol with Vitamin C and a control group did not receive any antioxidant. The clinical parameters
(heart rate, respiratory rate, systolic blood pressure) showed improvements with significant difference at the end of
study (post-intervention) as compared to admission (pre-intervention).Whereas, the sepsis (temperature, PCT, CRP
and WBC) and oxidative stress (8-OHdG/Creatinine) parameters showed improvements with significant difference at
the end of study (post-intervention) as compared to admission (pre-intervention). The TRI E group showed obvious
improvement in clinical, sepsis and oxidative stress parameters, as compared to TOCO and control groups. This
study showed that Vitamin E Tocotrienol and Vitamin E Tocopherol in combination with Vitamin C demonstrated
significant improvement in the clinical and laboratory parameters during the management of SIRS. Therefore,
Vitamin E in combination with Vitamin C had therapeutic benefits in the treatment of critically ill patients with
SIRS.
Tocotrienols
2.Genipin-Crosslinked Gelatin Scaffold in Tissue Engineering: A Systematic Review
MUHAMMAD MIOR AMIRUL A1 ; MOHD HEIKAL MY ; MH BUSRA F
Medicine and Health 2019;14(2):1-16
Gelatin sering digunakan dalam pembuatan kerangka kejuruteraan tisu kerana ia
mempunyai ciri-ciri biologi yang baik, termasuk mempercepatkan penyembuhan
luka. Genipin, bahan semula jadi yang diperolehi dari tumbuhan Gardenia, terbukti
berkesan dalam memperkukuhkan ciri-ciri fizikokimia gelatin. Ulasan sistematik
ini melaporkan pengetahuan terkini mengenai pengunaan genipin sebagai agen
penyilangan gelatin. Dua pangkalan data elektronik telah digunakan, iaitu Scopus
dan MEDLINE melalui Ebscohost. Carian dilakukan untuk penerbitan antara Januari
1999 hingga Disember 2018 menggunakan kata kunci ‘gelatin’ dan ‘genipin’.
Makalah berbahasa Inggeris, yang melaporkan kegunaan genipin untuk pembuatan
span gelatin telah dipilih. Terdapat 830 makalah dijumpai melalui carian kata
kunci di mana 14 makalah telah dipilih dan dibincangkan dalam ulasan sistematik
ini. Dapatan kajian termasuk kepekatan, suhu penyilangan, dan cara pembuatan
yang optima untuk genipin. Kepekatan genipin yang optima adalah 0.5% dan suhu
penyilangan yang optima adalah 25˚C. Keputusan kajian ini menunjukkan jurang
pengetahuan dalam pengunaan genipin sebagai agen penyilangan gelatin dan
lebih kajian diperlukan untuk mengisi jurang ini. Kajian ini menyediakan tinjauan
meluas berkenaan pengetahuan terkini mengenai penggunaan genipin sebagai
agen penyilangan gelatin.
3.A scanning electron microscopic study of in vivo tissue engineered respiratory epithelium in sheep.
Heikal MY ; Aminuddin BS ; Jeevanan J ; Chen HC ; Sharifah S ; Ruszymah BH
The Medical Journal of Malaysia 2008;63 Suppl A():34-34
Normal tracheal mucociliary clearance is the key to maintaining the health and defense of respiratory airway. Therefore the present of cilia and mucous blanket are important for tracheal epithelium to function effectively. In the present study, we prepared a tissue engineered respiratory epithelium construct (TEREC) made of autologous respiratory epithelium cells, fibroblast and fibrin from sheep owns blood which replaced a created tracheal mucosal defect. Scanning electron microscopy (SEM) showed encouraging result where immature cilia were present on the surface of TEREC. This result indicates that engineered respiratory epithelium was able to function as normal tissue.