Objective To study the safety and efficacy of endovascular therapy in the treatment of complicated extracranial carotid stenosis in patients with high surgical risk. Methods Seven patients with high surgical risk of complicated extracranial carotid stenosis received endovascular stenting. Results The stents were accurately implanted,and total disappearance of stenosis was obtained in 4 patients and the degree of stenosis reduced more than 90% in other 3 patients.The clinical symptoms were improved significantly or disappeared after the procedure without any complications. Follow up from 3 to 23 months,no restenosis and ischemic symptoms were noted in all cases. Conclusions Endovascular stenting is a safe and valid method of choice for the treatment of complicated extracranial carotid stenosis in high surgical risk patients.

OBJECTIVEThe effect of treated dentin matrix (TDM) to the proliferation and osteogenesis differentiation of bone marrow mesenchymal stem cells (BMSCs) is evaluated in vitro.

METHODSTDM leaching solution was prepared by dentine particles suffering from gradient demineralization. Human BMSCs were isolated and cultivated, and subsequently cultivated in the TDM leaching solution. The proliferation of BMSCs was detected by CCK-8. The osteogenesis-related proteins, including collagen type I (Col I) and runt-related transcription factor-2 (Runx2), were extracted and detected by Western blot after a 7-day culture.

RESULTSCompared with the control group and hydroxyapatite (HA)/β-tricalcium phosphate (βTCP) group, the proliferation of BMSCs cultivated in TDM leaching solution was significantly improved. The expression of Col I and Runx2 obviously increased after the 7-day cultivation in TDM leaching solution.

CONCLUSIONTDM can promote the proliferation and osteogenesis differentiation of BMSCs, implying the feasibility of the application in bone tissue engineering.

Bone Marrow Cells ; physiology ; Bone and Bones ; Calcium Phosphates ; Cell Differentiation ; Collagen Type I ; Core Binding Factor Alpha 1 Subunit ; Dentin ; physiology ; Hematopoietic Stem Cells ; Humans ; Mesenchymal Stromal Cells ; physiology ; Osteogenesis ; Tissue Engineering

Objective To compare the efficacy and safety of tranexamic acid(TA)and norethisterone(NET)for the treatment of patients with ovulatory menorrhagia in China. Methods Onehundred and thirty one patients with proven ovulatory menorrhagia from gynecologic clinics of 5 teaching hospitals located in 4 different cities in China were enrolled during Jul 2004 to Dec 2006.Ameng them 128 completed the study.Patients were randomly divided into two therapeutic regimen groups:TA 1g thrice daily during menstrual cycle days(D)1-5,69 cases;or NET 5 mg twice daily on D19-26.59 cases.The drugs were administered for 2 consecutive cycles,then withdrawn and patients were followed-up for 1 more cycle.Data on menstrual blood loss [ estimated by pictorial blood assessment chart(PBAC)],length of menstrual periods,quality of life(QOL)evaluated by a 6 item health-related questionnaire were collectedbefore,during each cycle and were compared.Results Both treatments led to significant decreases of mean PBAC scores and shorter duration of menstrual periods,and improved the QOL ranking during the twotreatment cycles.The mean percentages of PBAC decrements in the TA first and second cycles were significantly greater than those in the NET corresponding cycles(35%VS 17%,P=0.004;4J4%VS 34%,P=0.04 respectively).The success rate of TA second cycle was higher than that of the NET second cycle (41%VS 24%,P=0.04).Improvement of QOL ranking in the TA first cycle was also significantly better than those in the NET first cycle ( P=0.03).The percentage of patients with at least 1 adverse event in TA group(19%)was significantly lower than that in NET group(35%,P=0.04).Patients'willingness tocontinue the treatment in the TA second and follow-up cycles(94%,79%respectively)were significantly higher than those in the corresponding cycles of NET groups(79%,59%respectively;P=0.01,P=0.02).Conclusion The regimen of TA 3 g daily during menstrual days 1-5 is a more effective and tolerable treatment than luteal phase norethisterone for patients with ovulatory menorrhagia.

The pathological scars produced by scar hyperplasia are highly invasive, prone to recurrence, and often accompanied by itching and pain, which impose impact on patients’quality of life, physical and mental health. At present, it is generally believed that preventing the formation or recurrence of scars is the optimal way to deal with pathological scars. As a result, research on scar prevention is more worthy of attention. The author has collected relevant and instructive articles in the last 5 years and sorted them out by anti-scarring drugs, radiotherapy, laser therapy, in vitro non-invasive methods, discoveries and new clinical techniques, etc. All referenced articles focus on the prevention of scar hyperplasia and are closely connected with clinical practice. Summarization of the latest research and progress in this area has great significance for plastic surgeons.

The pathological scars produced by scar hyperplasia are highly invasive, prone to recurrence, and often accompanied by itching and pain, which impose impact on patients’quality of life, physical and mental health. At present, it is generally believed that preventing the formation or recurrence of scars is the optimal way to deal with pathological scars. As a result, research on scar prevention is more worthy of attention. The author has collected relevant and instructive articles in the last 5 years and sorted them out by anti-scarring drugs, radiotherapy, laser therapy, in vitro non-invasive methods, discoveries and new clinical techniques, etc. All referenced articles focus on the prevention of scar hyperplasia and are closely connected with clinical practice. Summarization of the latest research and progress in this area has great significance for plastic surgeons.

Objective:To investigate the characteristics of inflammatory response in BALB/cA-nu mice induced by oral 1, 2-dichloropropane (1, 2-DCP) , and to provides theoretical reference for further study of subchronic, chronic toxicity and carcinogenic mechanism.Methods:From October 2018, Clean grade healthy BALB/cA-nu mice were randomly divided into 5 groups with 10 mice in each group. And 860, 1150, 1500, 1950, 2535 mg/kg 1, 2-DCP were given by gavage respectively. Meanwhile, blank group and solvent control group (corn oil) were set up. Blood samples were collected from eyeballs and liver and bile tissues were collected for histopathological examination within 24 hours after exposure. The expression of alanine aminotransferase (ALT) , total bilirubin (TBLI) , C-reactive protein (CRP) , interleukin-6 (IL-6) , tumor necrosis factor-α (TNF-α) and tumor necrosis factor-β (TNF-β) were detected by enzyme-linked immunosorbent assay.Results:With the increase of the dose of 1, 2-DCP, the number of microbubbles in liver cells and the infiltration of inflammatory cells increased gradually. No pathological changes were found in the gallbladder. Compared with the blank group and solvent control group, the content of serum ALT in each exposure group was increased, the serum levels of IL-6 and TNF-β in 860, 1150, 1950 and 2350 mg/kg exposure groups were increased, the serum TNF-α and TBLI levels in 1 950, 2535 mg/kg groups were significantly higher ( P<0.05) . The levels of ALT, TBLI and TNF-β in serum of female mice were significantly different ( P<0.05) . There were significant differences in ALT, TBLI, CRP, IL-6, TNF-α, TNF-β in serum of male mice ( P<0.05) . Conclusion:Oral 1, 2-DCP may cause acute liver injury in BALB/cA-nu mice and increase the expression of serum inflammatory factors. Moreover, the types of inflammatory factors activated in male mice are more than those in female mice.

Objective:To investigate the characteristics of inflammatory response in BALB/cA-nu mice induced by oral 1, 2-dichloropropane (1, 2-DCP) , and to provides theoretical reference for further study of subchronic, chronic toxicity and carcinogenic mechanism.Methods:From October 2018, Clean grade healthy BALB/cA-nu mice were randomly divided into 5 groups with 10 mice in each group. And 860, 1150, 1500, 1950, 2535 mg/kg 1, 2-DCP were given by gavage respectively. Meanwhile, blank group and solvent control group (corn oil) were set up. Blood samples were collected from eyeballs and liver and bile tissues were collected for histopathological examination within 24 hours after exposure. The expression of alanine aminotransferase (ALT) , total bilirubin (TBLI) , C-reactive protein (CRP) , interleukin-6 (IL-6) , tumor necrosis factor-α (TNF-α) and tumor necrosis factor-β (TNF-β) were detected by enzyme-linked immunosorbent assay.Results:With the increase of the dose of 1, 2-DCP, the number of microbubbles in liver cells and the infiltration of inflammatory cells increased gradually. No pathological changes were found in the gallbladder. Compared with the blank group and solvent control group, the content of serum ALT in each exposure group was increased, the serum levels of IL-6 and TNF-β in 860, 1150, 1950 and 2350 mg/kg exposure groups were increased, the serum TNF-α and TBLI levels in 1 950, 2535 mg/kg groups were significantly higher ( P<0.05) . The levels of ALT, TBLI and TNF-β in serum of female mice were significantly different ( P<0.05) . There were significant differences in ALT, TBLI, CRP, IL-6, TNF-α, TNF-β in serum of male mice ( P<0.05) . Conclusion:Oral 1, 2-DCP may cause acute liver injury in BALB/cA-nu mice and increase the expression of serum inflammatory factors. Moreover, the types of inflammatory factors activated in male mice are more than those in female mice.

The pathological scars produced by scar hyperplasia are highly invasive, prone to recurrence, and often accompanied by itching and pain, which impose impact on patients’quality of life, physical and mental health. At present, it is generally believed that preventing the formation or recurrence of scars is the optimal way to deal with pathological scars. As a result, research on scar prevention is more worthy of attention. The author has collected relevant and instructive articles in the last 5 years and sorted them out by anti-scarring drugs, radiotherapy, laser therapy, in vitro non-invasive methods, discoveries and new clinical techniques, etc. All referenced articles focus on the prevention of scar hyperplasia and are closely connected with clinical practice. Summarization of the latest research and progress in this area has great significance for plastic surgeons.

The pathological scars produced by scar hyperplasia are highly invasive, prone to recurrence, and often accompanied by itching and pain, which impose impact on patients’quality of life, physical and mental health. At present, it is generally believed that preventing the formation or recurrence of scars is the optimal way to deal with pathological scars. As a result, research on scar prevention is more worthy of attention. The author has collected relevant and instructive articles in the last 5 years and sorted them out by anti-scarring drugs, radiotherapy, laser therapy, in vitro non-invasive methods, discoveries and new clinical techniques, etc. All referenced articles focus on the prevention of scar hyperplasia and are closely connected with clinical practice. Summarization of the latest research and progress in this area has great significance for plastic surgeons.

Objective:To explore the effect of a pelvis-assisting rehabilitation robot on lower limb function and knee proprioception after cerebral infarction.Methods:Forty hemiplegic cerebral infarction survivors were randomly divided into an experimental group and a control group, each of 20. Both groups were given routine neurological medication and rehabilitation training, while the experimental group was additionally provided with 20 minutes of robot-assisted gait training daily, five times a week for four weeks. Before and after the intervention, motor function, balance, walking function and knee joint proprioception were assessed using the simplified Fugl-Meyer lower limb assessment, the Berg balance scale, functional ambulation categorization and the Humac Norm isokinetic tester.Results:After the treatment, significant improvement was observed in all of the above measurements in both groups, but the improvements were significantly greater in the experimental group.Conclusions:Robotic pelvic assistance can effectively improve lower limb motor function, balance, knee proprioception and walking after cerebral infarction.