Gene expression of nm23 has been investigated in many kinds of tumors, including breast cancers, colon cancers, hepatocellular carcinomas, papillary carcinomas of the thyroid and malignant melanomas since the nm23 was dislovered by Dr. Steeg as a tumor metastatic suppressor gene. Reduced expression of nm23 gene implicated in high metastatic potential in a variety of malignancies. However, there have been only a few reports on genetic alteration and expression of nm23 in human gastric carcinomas even though gastric carcinoma is a leading malignancy in Korea. In this study, we examined the expression of nm23 protein by immunohistochemistry in advanced and early gastric carcinomas, adenomas, matching normal mucosa to elucidate the role of nm23 in the development, progression and metastasis of human gastric carcinomas. The results are as follows; 1) Nm23 was expressed in 39 cases(69.6%) of 56 advanced gastric carcinomas. Among them, strong positive cases(grade 3) were 26(46.4%) and weak positive cases(grade 2) were 13(23.2%). 2) Nm23 expression was significantly different (P<0.05) depending on the site of the neoplasm. Antral carcinomas showed grade3 positivity in the 22/37 cases(60%), but carcinomas of the body showed negative (grade 1) result in about half(42.1%). 3) Nm23 expression was more intense in the neoplasm than normal mucosa.(67.9%) 4) Nm23 expression was not significantly related to the lymph node metastasis, invasion of lymphatics or veins and depth of invasion. 5) In the well differentiated carcinomas, grade3 were more common(64.0%). But in cases of signet ring cell carcinoma, many cases were negative(50.0%). 6) Nm23 expression rate and intensity was significantly increased from the normal mucosa to the gastric adenomas, early gastric carcinomas and advanced gastric carcinomas.
Humans ; Carcinoma, Hepatocellular ; Neoplasm Metastasis ; Breast Neoplasms

Diverticulum is considered as common lesion involving any gastrointestinal tract from the pharynx to therectum. We reviewed 5806 cases of upper GI series and 801 cases of Barium enema during the period from Jan. 1978to Dec. 1981 in the Departement of Radiology, school of medicine, JeonBug National University Hospital to analizediverticula of the entire GI tract. The results are follows : 1. Roetgen examination of 5806 esophagus, stomachs,duodenums and small bowels, and 801 colons during the past four years: diverticular of esophagus, 60 cases(1.0%);diverticula of stomach, 42 cases (0.7%); diverticula of duodenum, 358 cases (6.2%); diverticula of small bowel,20( 0.3%); diverticula of the colon, 26 cases (3.2%). The location of diverticula inorder of frequency wasduodenum, colon, esophagus, stomach, and small bowel. 2. the most common site of diverticula of each GI tract wasfollows : diverticula of the esophagus, middle portion (84.7%); diverticula of stomach, the cardia(59.5%);diverticula of the duodenum, second portion(81.3%); diverticula of small bowel, the jejunum(96.4%) especially thelarger percentage were observed at the upper jejunum near the ligament of Treitz; diverticula of the colon, rightsided colon(80.8%), the cecum and ascending colon(57.1%) 3. Diverticula may occur at any age. The majority ofdiverticula of the entire GI tract were observed over 40 years of age. Especialy in diverticula of duodenum andcolon, thir frequency increase with age. 4. Duodenal diverticula were observed more frequently in womanthan in manbut in diverticula of the esophagus, stomach, small bowel, and colon, male was more frequently affected. 5. Thesize of diverticula of entire GI tract was variable. The majoprity of diverticula of the esophagus, stomach,duodenum and small bowel were intermediate size (10-49 mm). Diverticula of the colon were usually smaller than 10mm. 6. Multiplicity of diverticula of entire GI tract was 16.2% of 506 cases. In diverticula of esophagus,stomach, duodenum and small bowel, single lesion was more frequently found. on the other hand, the majority ofdiverticula of the colon were found as multiple lesion(69.2%).
Barium ; Cecum ; Colon ; Diverticulum ; Duodenum ; Enema ; Esophagus ; Gastrointestinal Tract ; Hand ; Humans ; Jejunum ; Jeollabuk-do ; Ligaments ; Male ; Pharynx ; Stomach

Primary breast lesions diagnosed by fine needle aspiration cytology, confirmed by histologic examination were analyzed by morphometry to evaluate the difference between benign and malignant lesions, and the methods obtaining the sample. Four size factors and 5 form factors were evaluated in 22 fibroadenomas and 20 carcinomas by image analyzer(Zeiss Ibas 2000) using the H-E stained slides. Nuclear size was significantly larger in the carcinoma cells than fibroadenoma cells both in the cytology and biopsy specimens, but the form factors were not significantly different. Both fibroadenoma and carcinoma cells were significantly larger in cytologic smear than histologic section. The cells in the cytology were more regular and round than those in histology, but not statistically significant. Fibroadenomas having cellular proliferation and atypism exhibited larger size and more irregular nuclei than non-proliferative fibroadenoma, but not statistically significant. Therefore nuclear morphometric analysis can be a helpful method to diagnose the questionable breast lesions and is a method appropriate for use as a quality control procedure in the fine needle aspiration cytology.
Biopsy* ; Biopsy, Fine-Needle* ; Breast* ; Cell Proliferation ; Fibroadenoma* ; Quality Control

Hemangioma of the ovary is a very rare lesion, although the ovary itself is a highly vascularized organ. In the literature review, about 40 cases were reported all of which were small in size and they were usually identified incidentally. The age range spanned from 4 months to 81 years. Cavernous hemangioma was the most common histologic type. We experienced a case of cavernous hemangioma of the left ovary in a 26 year-old pregnant woman. The lesion was 8x6x2cm in size with well demarcated margin. The cut surface was purple to bluish red in color and had a spongy-like appearance. Microscopically, it was composed of dilated vascular spaces with a common wall.
Pregnancy ; Female ; Humans ; Hemangioma

Sternocostoclavicular hyperostosis is a benign ossifying diathetic disorder characterized by hyperostosis and soft tissue ossification of the clavicles, anterior portion of the first ribs, and manubrium, with variable hyperostosis or ankylosis in the spine and sacroiliac joints. A review of the literature and our own case describes the clinical findings and its characteristic features in RI, CT, and plain film.
Ankylosis ; Clavicle ; Hyperostosis ; Hyperostosis, Sternocostoclavicular* ; Manubrium ; Ribs ; Sacroiliac Joint ; Spine

Ki-1 monoclonal antibody is a well known marker for Reed-Sternberg cells in Hodgkin's disease, but also occasionally reacts with activated lymphoid cells of either benign or malignant nature. Recently, Ki-1 antibody positive Non-Hodgkin's lymphoma, usually of large cell and/or polymorphous type, has been reported in the lymph nodes, skin, soft tissue, and stomach, but not in the bone. We report a case of multifocal primary bone lymphoma in a seven-year old body involving the left shoulder and right frontal bone, which proved to be a large cell, polymorphous lymphoma, helper T-cell type expressing Ki-1 antigen.
Child ; Male ; Female ; Humans

Zinc is essential for the survival of all cells. Retinal pigment epithelium(RPE)and chorioretinal complex contains unusually high concentration of zinc. Recently, zinc has received special attention of clinicians for its possible association with certain ocular diseases such as age-related macular degeneration(ARMD). However, the cellular and molecular pathological correlates of zinc deficiency in retinal cells and RPE are scanty. Thus, we examined zinc deficiency-induced RPE pathology using a cell-permeant zinc chelator, TPEN. Exposure of human RPE cultures to TPEN induced death of RPE between 1 micrometer to 2 micrometer concentrations within 48h exposure. TPEN-induced cell death was completely blocked by coaddition of zinc. Addition of a broad-spectrum caspase inhibitor, zVAD-fmk and a caspase 3 inhibitor, DEVD-fmk markedly attenuated TPEN induced RPE death.Addition of a protein synthesis inhibitor cycloheximide attenuated TPEN-induced RPE death significantly. On the other hand, EGF, IGF-1, PDGF, MK-801, or trolox did not show any protective effect. Additionally, TUNEL and Hoechst staining revealed conspicuous internucleosomal DNA fragmentation. Furthermore, ultrastructural changes supported that TPEN-induced RPE death was apoptosis. The present study using human RPE cells provides a model for zinc depletion-induced apoptosis. Considering the clinical importance of ARMD, the model may provide useful insights into the pathogenic mechanisms of zinc deficiency-related degenerative eye conditions.
Apoptosis* ; Caspase 3 ; Cell Death ; Cycloheximide ; Dizocilpine Maleate ; DNA Fragmentation ; Epidermal Growth Factor ; Epithelial Cells* ; Hand ; Humans* ; In Situ Nick-End Labeling ; Insulin-Like Growth Factor I ; Pathology ; Retinal Pigment Epithelium ; Retinaldehyde* ; Zinc*

No abstract available.
Bacteria*

No abstract available.
Diabetes Mellitus, Type 1* ; Echocardiography*

The expression of the nuclear phosphoprotein p53, a product of tumor suppressor gene, has been noted in a number of human tumors as a tumor suppressor. nm23 is a gene associated with low tumor metastatic potential and has been proposed to be a metastasis suppressor gene. To assess the role of p53 and nm23 expression in colorectal tumorigenesis and the association with clinicopathological parameters, an immunohistochemical study for mutant p53 and nm23 was done using mouse monoclonal antibodies in 43 colorectal carcinomas, 55 tubular adenomas and corresponding normal mucosa. In the tubular adenomas, p53 expression was significantly correlated with the degree of atypism(p<0.05) but not with other variables as well as with nm23. In the colorectal carcinoma, there were evidence of some correlation between metastasis, laterality and p53; laterality, depth of invasion and nm23 expression, but without statistical significance. Other clinicopathologic features were not significantly correlated. In the aspect of 'adenoma-carcinoma sequence', normal mucosa was totally negative for both p53 and nm23, and they were increasingly expressed through tubular adenoma to carcinoma with statistical significance(p<0.05). Therefore, it is suggested that both p53 and nm23 expressions occur in and around the time of transition to carcinoma from adenoma but are not significantly associated with the infiltrative behavior and metastasis.
Adenoma* ; Animals ; Antibodies, Monoclonal ; Carcinogenesis ; Colorectal Neoplasms ; Genes, Tumor Suppressor ; Genes, vif ; Humans ; Mice ; Mucous Membrane ; Neoplasm Metastasis