1.Estimation of Genetic Variation of Korean Isolates of Phytophthora capsici by Using Molecular Markers.
Hee Youn CHEE ; Hyeong Jin JEE
Mycobiology 2001;29(1):43-47
Genetic diversity of 21 Korean Phytophthora capsici isolates was analyzed by using several molecular markers such as random amplified polymorphic DNA(RAPD), M-13, microsatellite and random amplified microsatellite sequences(RAMS). The overall average similarity coefficient among the isolates was 86% based on the combined data obtained by the molecular markers. No molecular markers were found to be associated with hosts or geographic regions. In addition to RAPD, analysis based on repeated sequences such as (GTG)5, M-13 and RAMS could be used to assess population structure of P. capsici.
Genetic Variation*
;
Microsatellite Repeats
;
Phytophthora*
2.A Case of Allergic Contact Dermatitis to Ribavirin.
Chee Won OH ; Mi Youn PARK ; Jeong Hee HAHM
Annals of Dermatology 1993;5(1):44-46
Allergic contact dermatitis to topical agents mostly results from vehicles and preservatives and rarely from the active ingredients. Ribavirin, an active ingredient of Viramid® cream, is a synthetic nucleoside derivative with broad spectrum activity against a wide variety of DNA and RNA viruses. We report an 18-year-old woman, who had a perioral edematous patch with exudative crusts after topical application of Viramid® cream (nsung pharmaceutical Co., Korea) for the treatment of herpes labiahs. A patch test showed that the sensitizer was ribavirin, the active ingredient of the antiviral agent, Viramid® cream.
Adolescent
;
Dermatitis, Allergic Contact*
;
DNA
;
Female
;
Humans
;
Patch Tests
;
Ribavirin*
;
RNA Viruses
3.A Case of Allergic Contact Dermatitis to Ribavirin.
Chee Won OH ; Mi Youn PARK ; Jeong Hee HAHM
Annals of Dermatology 1993;5(1):44-46
Allergic contact dermatitis to topical agents mostly results from vehicles and preservatives and rarely from the active ingredients. Ribavirin, an active ingredient of Viramid® cream, is a synthetic nucleoside derivative with broad spectrum activity against a wide variety of DNA and RNA viruses. We report an 18-year-old woman, who had a perioral edematous patch with exudative crusts after topical application of Viramid® cream (nsung pharmaceutical Co., Korea) for the treatment of herpes labiahs. A patch test showed that the sensitizer was ribavirin, the active ingredient of the antiviral agent, Viramid® cream.
Adolescent
;
Dermatitis, Allergic Contact*
;
DNA
;
Female
;
Humans
;
Patch Tests
;
Ribavirin*
;
RNA Viruses
4.In vitro Evaluation of the Antifungal Activity of Propolis Extract on Cryptococcus neoformans and Candida albicans.
Mycobiology 2002;30(2):93-95
The antifungal activities of propolis on Cryptococcus neoformans and Candida albicans were evaluated. In microbroth culture assay, the MIC (minimum inhibitory concentration) of propolis for C. neoformans and C. albicans were 2 and 16 mg/ml, respectively. In propolis-included solid medium assay, the MIC of propolis for C. neoformans and C. albicans were 4 and 16 mg/ml, respectively. Propolis showed fungicidal activity against C. neoformans, whereas propolis possesed fungistatic activity against C. albicans. The MFC (minimum fungicidal concentration) for C. neoformans was 8 mg/ml. Cell morphology of C. neoformans was affected by treatment of propolis. In scanning electron microscope, the appearance of cell rupture was observed.
Candida albicans*
;
Candida*
;
Cryptococcus neoformans*
;
Cryptococcus*
;
Propolis*
;
Rupture
5.In vitro Activity of Celery Essential Oil against Malassezia furfur.
Mycobiology 2009;37(1):67-68
Antifungal activity of celery essential oil against Malassezia furfur was investigated using broth microdilution and vapor contact methods. Potent antifungal activity was evident using both methods. Fungicidal activity was revealed in the vapor contact method.
Apium graveolens
;
Malassezia
6.In vitro Antifungal Activity of Limonene against Trichophyton rubrum.
Hee Youn CHEE ; Hoon KIM ; Min Hee LEE
Mycobiology 2009;37(3):243-246
In this study, the antifungal activities of limonene against Trichophyton rubrum were evaluated via broth microdilution and vapor contact assays. In both assays, limonene was shown to exert a potent antifungal effect against T. rubrum. The volatile vapor of limonene at concentrations above 1 microl/800 ml air space strongly inhibited the growth of T. rubrum. The MIC value was 0.5% v/v in the broth microdilution assay. The antifungal activity of limonene against T. rubrum was characterized as a fungicidal effect.
Cyclohexenes
;
Terpenes
;
Trichophyton
7.Antifungal Activity of Clove Essential Oil and its Volatile Vapour Against Dermatophytic Fungi.
Mycobiology 2007;35(4):241-243
Antifungal activities of clove essential oil and its volatile vapour against dermatophytic fungi including Candida albicans, Epidermophyton floccosum. Microsporum audouinii, Trichophyton mentagrophytes, and Trichophyton rubrum were investigated. Both clove essential oil and its volatile vapour strongly inhibit spore germination and mycelial growth of the dermatophytic fungi tested. The volatile vapour of clove essential oil showed fungistatic activity whereas direct application of clove essential oil showed fungicidal activity.
Candida albicans
;
Clove Oil
;
Epidermophyton
;
Syzygium*
;
Fungi*
;
Germination
;
Microsporum
;
Spores
;
Trichophyton
8.Fungistatic Activity of Kojic Acid Against Human Pathogenic Fungi and Inhibition of Melanin-production in Cryptococcus neoformans.
Mycobiology 2003;31(4):248-250
Kojic acid was investigated for its antifungal activity against the human pathogenic fungi including Candida albicans, Cryptococcus neoformans and Trichophyton rubrum. For C. albicans, C. neoformans and T. rubrum, the MIC (minimum inhibitory concentration) of kojic acid was 640, 80 and 160 microg/ml, respectively. In C. neoformans, melanin-producing yeast, kojic acid-treated nonmelanized cell was more susceptible to magainin than melanized cell, suggesting melanin give a protective function against microbial peptide.
Candida albicans
;
Cryptococcus neoformans*
;
Cryptococcus*
;
Fungi*
;
Humans*
;
Melanins
;
Trichophyton
;
Yeasts
9.Antifungal Activity and Inhibitory Modes of Volatile Vapours of Essential Oils.
Mycobiology 2004;32(2):102-104
Antifungal activities of volatile vapours of essential oils were investigated. Volatile vapours of test essential oils except Cedar-wood and Pachouri showed inhibitory activity against test fungi. Volatile vapours of Birch essential oils exhibited fungistatic activity against dermatophytic filamentous fungi while others did fungicidal activity. Spores of dermatophytic filamentous fungi are more susceptible to volatile vapours of essential oils than mycelia.
Betula
;
Fungi
;
Oils, Volatile*
;
Spores
10.Molecular Analysis of Exophiala Species Using Molecular Markers.
Hee Youn CHEE ; Yoon Kyoung KIM
Mycobiology 2002;30(1):1-4
Genetic relatedness of medically important Exophiala species such as E. dermatitidis, E. mansonii, and three E. jeanselmei varieties: jeanselmei, lecanii-corni, and heteromorpha was examined using PCR-RFLP (restriction fragment length polymorphism) of ribosomal DNA, M-13, (GTG)5, and nucleotide sequences of ribosomal ITS (internal transcribed space) II regions. Three E. jeanselmei varieties showing distinct band patterns for each DNA markers as well as different nucleotide sequences of ribosomal ITS II regions could be considered as a separate species. E. dermatitidis and E. mansonii demonstrated the identical band patterns of RFLP of ribosomal DNA, M-13, and (GTG)5 markers. However, nucleotides sequences of ribosomal ITS II region were different between these two species.
Base Sequence
;
DNA, Ribosomal
;
Exophiala*
;
Genetic Markers
;
Nucleotides
;
Polymorphism, Restriction Fragment Length