No abstract available.
Female ; Germ Cells* ; Neoplasms, Germ Cell and Embryonal* ; Ovary*

BACKGROUND: The granulocyte-specific antigen NA which exist specifically on neutrophils and eosinophils have its antigenic determinant on Fcgamma receptor III. The alloantibodies and autoantibodies against this antigen are responsible for neonatal alloimmune neutropenia, chronic benign autoimmune neutropenia of infancy and transfusion-related acute lung injury. After discovering of biallelic polymorphism of NA antigen, there have been attempts to replace technically cumbersome serotyping methods by DNA-typing. This study was performed to investigate the genotype frequency of NA antigen In Koreans by DNA typing. METHODS: Using genomic DNA extracted from venous blood of 200 Koreans, genotype of NA antigen system was determined through polymerase chain reaction with sequence-specific primers (PCR-SSP), and the frequency was compared with previous studies. The NA genotypes of seven individuals were compared with their known phenotypes. RESULTS: The genotype frequencies of NA1+NA2-, NA1+NA2+, and NA1-NA2+ were 28.5%, 59.0%, and 12.5% respectively. The gene frequencies calculated from genotype frequencies were 0.58 and 0.42 for NA1 and NA2 respectively. The NA genotypes of seven individuals were correlated precisely with their phenotypes. CONCLUSION: The gene frequency of NA1 in Koreans was higher than that of Caucasian. The genotype frequencies of NA antigen in Koreans were similar to those of the Japanese and Chinese. This study will serve as a basic data for the study of the patients with diseases associated with granulocyte-specific antigens in Koreans.
Acute Lung Injury ; Asian Continental Ancestry Group ; Autoantibodies ; DNA ; DNA Fingerprinting ; Eosinophils ; Gene Frequency ; Genotype* ; Humans ; Isoantibodies ; Neutropenia ; Neutrophils ; Phenotype ; Polymerase Chain Reaction ; Serotyping

No abstract available.
Female ; Humans ; Pregnancy ; Pregnancy Trimester, Second*

We compared the left ventricular ejection fraction by echocardiography with the ejection fraction determined by computerized radionuclide ventriculography in 59 patients who were admitted to the Soon Chun Hyang University Hospotal under the impression of cardiovascular disease from May 1984 to August 1985. The results were as follow : 1) In all patients the ejection fractions by both methods were significantly correlated with each other(r=0.77, P<0.001). 2) In 10 patients with ischemic heart disease the ejection fractions by both methods were not correlated significantly(r=0.42, P>0.10). 3) Except the 10 patients with ischemic heart disease, the ejection fractions by both methods were correlated significantly(r=0.87, P<0.001). 4) In conclusion, though the ejection fraction by the above 2 methods represent the left ventricular function well, the ejection fraction by echocardiography can not represent the left ventricular function precisely in patients in patients with ischemic heart disease because of abnormal left ventricular wall motion and left ventricular function more precisely than that by echocardiography.
Cardiovascular Diseases ; Echocardiography* ; Humans ; Myocardial Ischemia ; Radionuclide Ventriculography* ; Stroke Volume ; Ventricular Function, Left

Angioedma is a group of disorders with multifactorial etiology but a similar clinical expression, is characterized by swelling of subcutaneous or submucosal tissue. Systemic lupus erythematosus(SLE) is a chronic multi-systemtic disease characterized by inflammation and tissue damage resulting from deposition of auto-antibodies and immune complex. We experienced a case of angioedema which was the first manifestation of SLE in 24-year-old female patient. She had suffered from severe facial edema and multiple lymphadenopathy for six months and she also had pleural effusion, positive anti-nuclear and anti-DNA antibody test. Marked decrease of C3 and C4 levels was noted with normal antigenic level, and activity of Cl esterase inhibitor. The angioedema was not improved with anti-hitamine agents and instead disappeared with use of corticosteroid and non-steroidal anti-inflammatory drugs. Complement levels normalized after corticosteroid treament. We report a case of SLE which initial manifestation was angioedema.
Angioedema ; Antigen-Antibody Complex ; Complement System Proteins ; Edema ; Female ; Humans ; Inflammation ; Lupus Erythematosus, Systemic* ; Lymphatic Diseases ; Pleural Effusion ; Young Adult

Cisplatin has been widely used as chemotherapeutic agent for the treatment of cervical cancer, ovarian cancer, head and neck cancer and squamous cell carcinoma of lung. But cisplatin is highly toxic with nephrotoxicity, gastrointestinal toxicity, myelosuppression and neurotoxicity. The second generation drug of platinum compound, carboplatin was developed in 1980s to reduce side effects. Carboplatin has low nephrotoxicity but its major toxic effect is thrombocytopenia, In this study, the side effects of cisplatin and carboplatin were evaluated on 37 patients of cervical cancer in 169 chemotherpy cycles who were recieved combined VBP chemotherpeutic regimen consisting of cisplatin or carboplatin. Nephrotoxicity of grade 2 or over were 16% in cisplatin group and 1% in carboplatin group. Granulocytopenia of grade 2 or over were 34% in cisplatin group and 10% in carboplatin group. Thrombocytopenia of grade 2 or over were 7% in cisplatin group and 21% in carboplatin group. Gastrointestinal toxicity of grade 2 or over were 11% in cisplatin group and 0% in carboplatin group. This clinical study demonstrated that cisplatin has more toxic effects than carboplatin except thrombocytopenia.
Agranulocytosis ; Carboplatin* ; Carcinoma, Squamous Cell ; Cisplatin* ; Drug Therapy, Combination* ; Head and Neck Neoplasms ; Humans ; Lung ; Ovarian Neoplasms ; Platinum ; Thrombocytopenia ; Uterine Cervical Neoplasms

Serological prenatal screening tests are widely used to detect fetal chromosomal abnormalities such as Down and Edward syndromes. Amniocentesis is conducted as a confirmatory test in the screening-positive case. After discovering of presence of fetal cell-free DNA in maternal blood, non-invasive prenatal test (NIPT) coupled with next generation sequencing are performed in abroad. Results of genomics-based NIPT results supplied to Labgenomics laborotory from June, 2013 to August, 2014 were analyzed. Maternal blood samples were collected into specific Cell-Free DNA BCT tube and were transported. The samples were then delivered to Ariosa Diagnostics by FEDEX. Fetal cell-free DNA samples were analyzed using the Harmony test with sequencing of relevant chromosomes and by using the FORTE (fetal-fraction optimized risk of trisomy evaluation) algorism at Ariosa Diagnostics. In all, 149 cases from 28 medical clinics were analyzed. Six subjects were required recollection of samples because of a low fetal DNA fraction in the initially obtained samples. Of these 6 subjects, no sample could be collected from one. Of the remaining 148 cases, 144 had a low risk of trisomy, and 4 had a high risk for Down syndrome, thus providing a positivity percentage of 2.7%. Fetal DNA fraction in the maternal blood samples ranged from 4.2% to 23.7% with a mean value of 12.0%. We have experienced cases with a high risk for Down syndrome with genomics-based NIPT referred to abroad.
Amniocentesis ; Chromosome Aberrations ; DNA ; Down Syndrome ; Prenatal Diagnosis ; Trisomy

PURPOSE: This study aimed to identify relationships between compassion fatigue, burnout, and turnover intention in Korean hospital nurses. METHODS: In total, 142 hospital nurses were surveyed as part of data collection. Data related to compassion fatigue, burnout, and turnover intention were collected using a questionnaire between May 2011 and September 2011. The data analysis was performed using PASW 19.0 program, which included one-way ANOVA, independent t-tests, Pearson's correlation coefficient, and hierarchical regression analysis. RESULTS: This study detected a positive correlation between compassion fatigue and burnout(r=.37, p<.001), and turnover intention(r=.55, p<.001). Compassion fatigue accounted for 29.6% of the variance for turnover intention among Korean hospital nurses. CONCLUSION: The results indicate that it is necessary to reduce compassion fatigue, and turnover intention among Korean hospital nurses.
Adult ; Analysis of Variance ; Asian Continental Ancestry Group ; Burnout, Professional/*psychology ; *Fatigue ; Humans ; Nursing Staff, Hospital/*psychology ; *Personnel Turnover ; Questionnaires ; Republic of Korea

No abstract available.
Carcinoma, Squamous Cell* ; Liver*

A 29 year old woman with unresectable leiomyosarcoma of the inferior vena cave extending into the right atrium of the heart was presented. The upper and middle inferior vena cava involved and there were both intrinsic and extrinsic tumor growth components. The diagnosis was made by a combination of radiological studies including ultrasound, CT, inferior vena cavography and arteriography and the diagnosis was confirmed by ultrasound guided rumor biopsy. The clinical and radiological presenting features were reviewed.
Angiography ; Biopsy ; Diagnosis ; Female ; Heart ; Heart Atria ; Humans ; Leiomyosarcoma* ; Ultrasonography ; Vena Cava, Inferior*