1.Up-regulation of prothymosin alpha in THP-1 cells infected with Mycobacterium tuberculosis.
Ho Yeon SONG ; Kwang Sik JANG ; Hee Sun BYOUN ; Shin Je LEE ; Jin Koo KIM ; Yong Kyung CHOE ; Kwang Kjune KO
Journal of the Korean Society for Microbiology 2000;35(2):149-157
Mycobacterium tuberculosis is capable of growing and survival within macrophage. The purpose of this study was to identify the genes regulated by infection of mycobacteria in human monocytic THP-1 cells. We used the differential display reverse transcriptase polymerase chain reaction (DD RT-PCR) and nothern blot analysis to confirm the differentially expressed genes from THP-1 cells infected with live Mycobacterium tuberculosis H37Rv, heat-kille Mycobacterium tuberculosis H37Rv and live Mycobacterium bovis BCG. Among many up or down-regulated clones, 27 clones were sequenced and compared with known genes on GenBank. Thirteen of over-expressed clones from THP-1 cells infected with live Mycobacterium tuberculosis H37Rv were identical to human prothymosin alpha, eight were novel clones and six clones showed homology with Human ferritin H chain, Escherichia coli bgl, Mouse RNA-dependent EIF-2 alpha kinase, E. coli htrL, Hyaluronan receptor and T cell receptor. Our result suggests that Mycobacterium tuberculosis might regulate prothymosin alpha gene transcription in monocytic THP-1 cell.
Animals
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Antigens, CD44
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Clone Cells
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Databases, Nucleic Acid
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Escherichia coli
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Eukaryotic Initiation Factor-2
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Ferritins
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Humans
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Macrophages
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Mice
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Mycobacterium bovis
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Mycobacterium tuberculosis*
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Mycobacterium*
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Phosphotransferases
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Receptors, Antigen, T-Cell
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Reverse Transcriptase Polymerase Chain Reaction
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Up-Regulation*