1.Hemangiopercytoma of the Meninges: The immunohistochemical study for the relationship between hemangiopericytic meningioma and peripheral hemangiopericytoma.
Sun Hee YOON ; Weon Yeong CHOI ; Sook Nyo LEE ; In Sook LIM ; Sook Hee HONG
Korean Journal of Pathology 1990;24(4):502-508
Hemangiopericycic maningioma is clinically and pathologically similar to peripheral Hemangiopericytoma and now tends to be terned as hemangiopericytoma of central nervous system. The authors studied 3 cases of hemangiopericytic meningioma obtained from 3 patients, 1 case of meningotheliomatous meningioma, angioblastic meningioma and transitional meningioma, and 2 cases of peripheral hemangiopericytoma, which had operated from November 1988 to May 1989 at the department of neurosurgery, Pusan Inje University Hospital. The authors analysed and compared the immunohistochemical finding and light microscopic apearance. The results obtained were summarized as follows; 1) Classic maningioma (meningotheliomatous meningioma, transitional meningioma and angioblastic meningioma) shows reactivity to both EMA and vimentin. 2) Hemangiopercicytic meningioma and peripheral hemangiopericytoma are reactive only to vimentin, so the two tumors are suggested as same type of tumor. 3) One of 3 cases of hemangiopericytic meningioma shows whorling and interlacing bundles of spindle cells, the peculiar light microscopic features of transitional meningioma, suggesting transitional or mixed form of hemangiopericytic meningioma and transitional meningioma.
Meningioma
2.Hemangiopercytoma of the Meninges: The immunohistochemical study for the relationship between hemangiopericytic meningioma and peripheral hemangiopericytoma.
Sun Hee YOON ; Weon Yeong CHOI ; Sook Nyo LEE ; In Sook LIM ; Sook Hee HONG
Korean Journal of Pathology 1990;24(4):502-508
Hemangiopericycic maningioma is clinically and pathologically similar to peripheral Hemangiopericytoma and now tends to be terned as hemangiopericytoma of central nervous system. The authors studied 3 cases of hemangiopericytic meningioma obtained from 3 patients, 1 case of meningotheliomatous meningioma, angioblastic meningioma and transitional meningioma, and 2 cases of peripheral hemangiopericytoma, which had operated from November 1988 to May 1989 at the department of neurosurgery, Pusan Inje University Hospital. The authors analysed and compared the immunohistochemical finding and light microscopic apearance. The results obtained were summarized as follows; 1) Classic maningioma (meningotheliomatous meningioma, transitional meningioma and angioblastic meningioma) shows reactivity to both EMA and vimentin. 2) Hemangiopercicytic meningioma and peripheral hemangiopericytoma are reactive only to vimentin, so the two tumors are suggested as same type of tumor. 3) One of 3 cases of hemangiopericytic meningioma shows whorling and interlacing bundles of spindle cells, the peculiar light microscopic features of transitional meningioma, suggesting transitional or mixed form of hemangiopericytic meningioma and transitional meningioma.
Meningioma
3.A Study on Nutrient Intake, Food Behavior and Health Conditions according to Food Intake Diversity in the Elderly in a Local City.
In Sook KIM ; Hyeo Hee YU ; Yoon Sook KIM
Korean Journal of Community Nutrition 2001;6(2):205-217
This survey was carried out to study the difference of nutrient intake, food behavior and health condition according to food intake diversity in the elderly(age 65 year over and 74 year under) in a local city. A diet survey with one day 24-recall method was used for 216 subjects(65 male & 151 female). Based on a food intake diversity examination, including an evaluation of KDDS(Korean's Dietary Diversity Score ; counts the number of food groups consumed daily from total five food groups(cereal, meat, vegetable, dairy, and oil groups)), Meal Balance(apply the KDDS at breakfast, lunch and dinner), and DVS(Dietary Variety Score ; total number of foods consumed), subjects were clustered according to food intake diversity. Food intake diversity patterns of male and female clusters were classified each into threes(Male-low group(24.6%), middle group(33.8%), and high group(41.5%)/Female low group(41.7%) middle group(37.1%), and high group(21.2%). The averages of KDDS, Meal Balance and DVS were 3.0, 7.5, 14.8 in the male middle group, and 4.5, 10.2, 25.7 in the male high group, 2.7, 6.3, 14.3 in the female low group, 4.0, 8.6, 18.0 in the female middle group, and 4.5, 10.5, 25.7 in the female high group respectively. The average dairy intake of nutrients below the RDA were energy, protein, Ca, Fe, Vitamin A, Vitamin B2 and niacin in the male low group, energy, protein, Ca, Fe, Vitamin A, Vitamin B2 in the male middle group and energy, Ca, Vitamin B2 in the male high group but a little. Also the female low group had intakes of all nutrients except Vitamin C, the middle group had intakes of energy, protein, Ca, Fe, Vitamin A, Vitamin B2, and the female high group had intakes of energy, Ca, Vitamin A below the RDA respectively. Food intake diversity increasing, nutrient intake, food behavior and health condition proved to be good. This study explored the usefulness of cluster analysis in identifying food intake diversity of three groups of the elderly in relation to their nutrient intake, food behavior and health conditions.
Aged*
;
Ascorbic Acid
;
Breakfast
;
Diet Surveys
;
Eating*
;
Female
;
Humans
;
Lunch
;
Male
;
Meals
;
Meat
;
Niacin
;
Riboflavin
;
Vegetables
;
Vitamin A
4.The Effect of Unilateral Orbital Enucleation on Acetylcholine and Muscarinic Receptors in Visual Pathway, and Physical Activity of Rats.
Yoon Hee KIM ; Young Sook PAE ; Byung Chae CHO
Journal of the Korean Ophthalmological Society 1995;36(5):855-867
Acetylcholine is one of the major neurotransmitters in visual system of rats. Cholinergic, serotonergic and gamma-aminobutyric acid(GABA) ergic projections are known as modulators of visual processing. The purpose of this study was to examine the effects of unilateral orbital enucleation on the amount of acetylcholine and its receptor bindings in dorsolateral geniculate body, superior colliculus and visual cortex of rats, and on motor activites. The amount of acetylcholine was measured by gas chromatography; whereas [3H]quinuclidinyl benzilate(QNB) bindings to muscarinic receptors were examined by autoradiography. Motor activity was measured by activity meter for 24 hours. The results of this study were as follows: 1. The amount of acetylcholine in superior colliculus significantly increased at 1 week after enucleation, but it decreased at 2 and 4 weeks after enucleation. In visual cortex, the amount of acetylcholine did not change at 1 week after enucleation, but it significantly decreased at 2 and 4 weeks after enucleation. 2. After enucleation, [3H]-QNB binding to muscarinic receptors gradually decreased over the 4 weeks in dorsolateral geniculate body, superior colliculus and visual cortex. 3. Locomotor activity of rats did not show any change at 1 week after enucleat ion, but it decreased at 2 and 4 weeks after enucleation. In conclusion, unilateral orbital enucleation resulted in a reduction in the amount of acetylcholine and muscarinic receptors in rat visual system, which was speculated to be a major cause of a decrease in physical activity.
Acetylcholine*
;
Animals
;
Autoradiography
;
Chromatography, Gas
;
Motor Activity*
;
Neurotransmitter Agents
;
Orbit*
;
Rats*
;
Receptors, Muscarinic*
;
Superior Colliculi
;
Visual Cortex
;
Visual Pathways*
5.Antigen analysis of rickettsia typhi isolated in Korea: SDS-PAGE and immunoblotting characters.
Min Kee CHO ; Chang Soon YOON ; Yoon Won KIM ; Hee Sook KIM ; Chang Hong MIN
Journal of the Korean Society for Microbiology 1992;27(5):427-434
No abstract available.
Electrophoresis, Polyacrylamide Gel*
;
Immunoblotting*
;
Korea*
;
Rickettsia typhi*
;
Rickettsia*
6.A Case of Duplication o f the Ileum.
Sang Kenn OH ; Sook Young LEE ; Yoon Oh PARK ; Mi Sook PARK ; Young Chul LEE ; Hee Dae PARK
Journal of the Korean Pediatric Society 1987;30(12):1446-1451
No abstract available.
Ileum*
7.Comparative Analysis Research of Inpatient Satisfaction with Nursing on Comprehensive Nursing Service Units & General Units and Nurses' Work Stress.
Journal of Korean Academy of Nursing Administration 2017;23(3):229-238
PURPOSE: The purpose of this study was to provide basic research material necessary for the establishment of comprehensive nursing service units, through a comparative analysis of inpatient satisfaction with nursing between comprehensive nursing service and general units and the work stress of nurses. METHODS: The survey participants were inpatients and nurses from 3 general hospitals in Busan, including 6 units, 123 nurses and 220 patients. Date collection was done through March and April 2016. Collected data were analyzed using test and independent t-test with SPSS 23 Win program. RESULTS: Inpatients on comprehensive nursing service units had higher scores for satisfaction than inpatients on general units. There was no statistically significant difference in nurses' work stress between the two types of units. CONCLUSION: The findings indicate that although inpatient satisfaction with comprehensive nursing service units is higher than for general units, work stress for nurses is comparatively high for both types of units. While it is necessary to expand the implementation of comprehensive nursing service units, an institutional strategy for reducing nurses' work stress is required if the expansion is to be successful.
Busan
;
Hospitals, General
;
Humans
;
Inpatients*
;
Nursing Services*
;
Nursing*
8.Identification of Oocyte-Specific Diva-Associated Proteins using Mass Spectrometry.
Se Jin YOON ; Jung Woong KIM ; Kyung Hee CHOI ; Sook Hwan LEE ; Kyung Ah LEE
Korean Journal of Fertility and Sterility 2006;33(3):189-198
OBJECTIVE: We previously described that Diva is highly expressed in matured metaphase II (MII) oocytes compared to immature germinal vesicle (GV) oocytes in mouse.1 We report here that the expression of Diva transcript as well as protein is oocyte-specific. To elucidate its physiological role in oocyte, the binding partner(s) of Diva has been identified by using immunoprecipitation (IP) followed by Mass Spectrometry. METHODS: NIH/3T3 cells were transiently transfected for 24 h with either empty vector for control or FLAG-tagged mouse Diva construct, and IP was performed with anti-FLAG antibody. The immuno-isolated complexes were resolved by SDS-PAGE on a 12% gel followed by Coomassie Blue staining. For in-gel digestion, 15 bands of interest were excised manually and digested with trypsin. All mass spectra were acquired at a positive reflector mode by a 4700 Proteomics Analyzer (Applied Biosystems, Framingham, MA). Proteins were identified by searching the NCBI nonredundant database using MASCOT Peptide Mass Fingerprint software (Matrixscience, London). RESULTS: Diva-associated complexes were formed in FLAG-tagged mouse Diva-overexpressed NIH/3T3 cells via IP using anti-FLAG-conjugated beads. Among the excised 15 bands, actin and actin-binding proteins such as tropomyosin, tropomodulin 3, and alpha-actinin were identified. Binding between Diva and actin or tropomyosin was confirmed by IP followed by Western blot analysis. Both bindings were also detected endogenously in mouse ovaries, indicating that Diva works with actin and tropomyosin. CONCLUSIONS: This is the first report that immuno-isolated Diva-associated complexes are related to actin filament of the cytoskeletal system. When we consider the association of Diva with actin and tropomyosin, oocyte-specific Diva may play a role in modulating the cytoskeletal system during oocyte maturation.
Actin Cytoskeleton
;
Actinin
;
Actins
;
Animals
;
Blotting, Western
;
Dermatoglyphics
;
Digestion
;
Electrophoresis, Polyacrylamide Gel
;
Female
;
Immunoprecipitation
;
Mass Spectrometry*
;
Metaphase
;
Mice
;
Microfilament Proteins
;
Oocytes
;
Ovary
;
Proteomics
;
Tropomodulin
;
Tropomyosin
;
Trypsin
9.Comparision of Three Trichomonas Antigens for the Detection of IgG Antibody in Serum.
Jae Sook RYU ; Kyong YOON ; Seo Eun HA ; Duk Young MIN ; Myoung Hee AHN
Korean Journal of Clinical Microbiology 2000;3(1):62-68
BACKGROUND: Direct wet mount examination of vaginal secretion, widely applied for the diagnosis of Trichomonas vaginalis infection in woman patients, is rapid and economical. However, the sensitivity of this technique is not so high. In this study enzyme-linked immunosorbent assay(ELISA) was employed for the detection of serum anti-T. vaginalis IgG antibodies from vaginal trichomoniasis patients. METHODS: Eighty sera from trichomonoasis patients who visited a Dr. Yoon Kyong's Obstetric & Gynecologic Clinic in Songnam and 30 non-infected healthy men were tested for detection of anti-T. vaginalis IgG antibody. Soluble lysate and excretory-secretory antigen prepared by mixing of six isolates of T. vaginalis, and lysate from one isolate(KT4) were used as antigen for ELISA. RESULTS: The sensitivity of ELISA using lysate of six isolates was 95.0%, and the sensitivity of the lysate from KT4 and mixed excretory-secretory antigen from 6 isolates were 86.4% and 76.3%, respectively. Specificities of ELISA by three 93.3%, 96.3% and 92.0%, respectively. CONCLUSION: It is suggested that ELISA using mixed lysate of T. vaginalis six isolates could be useful tools for the diagnosis of trichomoniasis.
Antibodies
;
Diagnosis
;
Enzyme-Linked Immunosorbent Assay
;
Female
;
Gyeonggi-do
;
Humans
;
Immunoglobulin G*
;
Male
;
Trichomonas vaginalis
;
Trichomonas*
10.Exceptionally Good Lymphocytic Infiltration with Histiocytes and Multinucleated Giant Cells of Stomach Cancer: A case report.
Dongsoo SUK ; Sook Hee HONG ; Hye Kyung YOON ; Hyung Gin KANG
Korean Journal of Pathology 1986;20(1):112-115
Stomach of 34 year old man showed an early stage of the cancer with slight involvement of the superficial part of the inner muscle layer and accompanied with one metastatic lymph node. The cancer is that of medium differentiated adenocarcinoma. There is an heavy infiltration of lymphocytes mixed with histiocytic mononuclear cells and multinucleated giant cells. Some giant cells appear as Langhans' type suggesting phagocytic cells of their origin containing PAS positive materials in the cytoplasma. In other places, they appear as atrophic cancer nests suggesting that these tumor nests were arrested and undergone to regressive cellular process because of the over-whelming immunological pressure by the host.
Male
;
Humans
;
Neoplasm Metastasis
;
Stomach Neoplasms