1.Isolation of Echovirus Serotype 25 from Patient with Hand , Foot and Mouth Disease in Pusan , 1998.
Young Hee KIM ; Kyung Soon CHO
Journal of the Korean Society for Microbiology 1999;34(2):157-162
Identification of virus is very importnant in aspects of preventive surveillance system of viral infection. The first isolation of echovirus serotype 25 from hand, foot and mouth disease was accomplished in Pusan, 1998. The authors determined some properties of this virus. Two cases of outbreak were confirmed from the stools of a 3 year-old girl and one 4 year-old boy suffering from hand, foot and mouth disease. Both occurred in April. The isolated viruses showed strong cytopathic effect on RD cells, also on HEp-2, and Vero cell lines after 3 days at 34'C, CO incubation. Isolated virus was identified as echovirus serotype 25 by neutralizing antibody test. Electron micrograph of negative-stained echovirus serotype 25 showed non-enveloped, isometric particle and about 30 nm in diameter.
Animals
;
Antibodies, Neutralizing
;
Busan*
;
Child, Preschool
;
Enterovirus B, Human*
;
Female
;
Foot*
;
Foot-and-Mouth Disease*
;
Hand*
;
Hand, Foot and Mouth Disease
;
Humans
;
Male
;
Vero Cells
2.CT findings of thoracic involvement of lymphoma.
Hee Jin KIM ; Kyu Ok CHOE ; Hee Kyung CHO
Journal of the Korean Radiological Society 1993;29(2):223-229
Chest CT scans of 70 patents with malignant lymphoma were reviewed to evaluate the thoacic manifestation of malignant lymphoma. Sixteen patients had Hodgkin's disease and 54 patients had non-Hodgkin's lymphoma. The thoracic involvement of malignant lymphoma was observed i 47 patients (67.1%) and 11 of these patients had Hodgkin's disease, and 36 had non-Hodgkin's lympoma. The most common finding was mediastinal lymphadenopathy and the most frequently involved sites were anterior mediastinal and paratracheal lymph nodes. Pulmonary parenchymal involvement was seen in 11 patients (15.7%), and CT scan showed thickening of bronchovascular bundle, parenchymal consolidation and nodules. Pleural effusion was seen in 18 patients (25.7%), however, without any evidence of parietal pleural thickening in all cases. Involvement of chest wall and breast was seen in two patient(2.9%). The data obtained through the current study showed no differences from those of previous reports, except the fact that there was no CT evidence of pleural thickening in patients who had pleural effusion.
Breast
;
Hodgkin Disease
;
Humans
;
Lymph Nodes
;
Lymphatic Diseases
;
Lymphoma*
;
Lymphoma, Non-Hodgkin
;
Pleural Effusion
;
Rabeprazole
;
Thoracic Wall
;
Tomography, X-Ray Computed
4.Renal and hormonal effects of lithium chloride.
Nam Jin PARK ; Suhn Hee KIM ; Kyung Woo CHO
Journal of Korean Society of Endocrinology 1992;7(3):251-259
No abstract available.
Lithium Chloride*
;
Lithium*
5.Overview of Roles for Non-cardiac Natriuretic Peptides: Roles in Neural, Endocrine and Immune Systmes.
Kyung Woo CHO ; Suhn Hee KIM ; Sung Joo KIM
Journal of Korean Society of Endocrinology 2000;15(6):760-778
No Abstract Available.
Natriuretic Peptides*
6.Esophageal Actinomycosis after Insertion of Esophageal Stent: A Case of Surgical Experience.
Sung Rae CHO ; Hyun Woo SHIN ; Hee Kyung CHANG
The Korean Journal of Thoracic and Cardiovascular Surgery 2000;33(7):601-604
Actinomycosis is an indolent, suppurative infection caused by an anaerobic gram-positive organism (usually actinomyces israelii) which usually causes infection in the face, mediastitum, lung, and abdomen. Primary esophageal actinomycosis which is not related with pulmonary or mediastinal actinomycosis, is very rare, especially in immunocompetent host. A 58-year-old woman has been suffered from dysphagia, odynophagia, and chest pain after insertion of esophageal stent in esophageal acid stricture. She underwent a esophagectomy with esophagogastrostomy for above mentioned symptoms. Pathologic diagnosis was a esophageal actinomycosis.
Abdomen
;
Actinomyces
;
Actinomycosis*
;
Chest Pain
;
Constriction, Pathologic
;
Deglutition Disorders
;
Diagnosis
;
Esophagectomy
;
Female
;
Humans
;
Lung
;
Middle Aged
;
Stents*
7.Concentration of IL-1B, IL-6, IL-8, TNF-a in Cerebrospinal Fluid of Patients with Meningitis and Control.
Myung Woong CHANG ; Dong CHO ; Kyung Hee KANG
Korean Journal of Immunology 1999;21(2):99-107
This study was conducted to determine the level of inflammatory cytokines in the cerebrospinal fluid (CSF) of patients with meningitis. All the CSF of the patients were examined by Gram and acid-fast stain, culture, and PCR for Mycobacterium tuberculosis and Mycoplasrma spp..The levels of sugar, protein and leukocytes count were also evaluated in the CSFs. Concentrations of Interleukin (IL)-1B, IL-6, IL-8, tumor necrosis factor (TNF)-a in the CSF were evaluated by the ELISA kit (Genzyme, USA). General bacteria, tubercle bacilli, and Mycoplasma spp. were not detected with stain and culture methods, but, Mycoplasma spp. was detected by PCR method from four (6.3%) patients with meningitis. The mean CSF concentration of IL-1B, IL-6, IL-8, and TNF-cx in the control group were 0.6+/-0.2, 896.8+/-107.6, 50.1+/-5.1, and 4.8+/-1.4 pg/ml, respectively. The mean CSF concentration of IL-1B, IL-6, IL-8, and TNF-a in the patients with aseptic meningitis were 3.8+/-0.6, 1261.6+/-144.3, 466.7+/-42.3, and 10.8+/-2.0 pg/ml, respectively. The mean CSF concentration of IL-1B, IL-6, IL-8, and TNF-a in the patients with mycoplasmal meningitis were 10.2+/-8.1, 1979.5+/-133.8, 459.7+/-96.4, and 17.5+/-5.1 pg/ml, respectively. There were significantly differences in the levels of IL-1B, IL-6, IL-8, and TNF-a between control and patients with aseptic meningitis or Mycoplasmal meningitis (each p<0.001). These results suggest that increased levels of IL-1B, IL-8, and TNF-a could be higly suggestive of meningitis.
Bacteria
;
Cerebrospinal Fluid*
;
Cytokines
;
Enzyme-Linked Immunosorbent Assay
;
Humans
;
Interleukin-6*
;
Interleukin-8*
;
Interleukins
;
Leukocytes
;
Meningitis*
;
Meningitis, Aseptic
;
Mycobacterium tuberculosis
;
Mycoplasma
;
Polymerase Chain Reaction
;
Tumor Necrosis Factor-alpha
8.Prevalence Rate of Irregular Antibodies in Transfusion Candidates and Pregnant Women in Korea.
Kyung Mi CHOI ; Dong Hee CHO ; Hyun Ok KIM
Korean Journal of Clinical Pathology 1997;17(5):847-852
BACKGROUND: The authors studied to estimate the frequency of irregular antibodies and their significance in blood transfusion and antenatal care in Korea. METHODS: Irregular antibodies were tested by immediate saline spin, 37degrees C albumin and antiglobulin test for 2,008 transfusion candidates and 1,047 pregnant women at Severance hospital using commercial screening and identification cells (Dade, U.S.A.). RESULTS: The irregular antibodies were detected in 38 (1.24%) of total 3,055 subjects (transfusion candidates: 0.9%, pregnant women: 1.91%) . In transfusion candidates, the detected antibodies were Lewis antibodies, cold antibodies (anti-M, anti-P 1), Rh antibodies and unspeified warm antibodies, and their distributions were 56%, 22%, 17%, and 5%, respectively. In pregnant women, the detected antibodies were Lewis antibodies, Rh antibodies, anti-Jra, and unspeified warm antibodies, and their distributions were 45%, 45%, 5% and 5%, respectively. At immediate saline phase, 58% of irregular antibodies were detected. At 37degrees C albumin phase, 90% of irregular antibodies were detected and only 10% of irregular antibodies were detected at antiglobulin phase. CONCLUSIONS: Although the prevalence rates of clinically important irregular antibodies were low, 1/1000 of irregular antibodies could not be detected. Therefore, irregular antibody screening should be performed in all pretransfusion test. And, if antibody detection tests are negative, immediate saline crossmaching methods are acceptable in Korea.
Antibodies*
;
Blood Transfusion
;
Coombs Test
;
Female
;
Humans
;
Korea*
;
Mass Screening
;
Pregnant Women*
;
Prevalence*
9.Prevalence Rate of Irregular Antibodies in Transfusion Candidates and Pregnant Women in Korea.
Kyung Mi CHOI ; Dong Hee CHO ; Hyun Ok KIM
Korean Journal of Clinical Pathology 1997;17(5):847-852
BACKGROUND: The authors studied to estimate the frequency of irregular antibodies and their significance in blood transfusion and antenatal care in Korea. METHODS: Irregular antibodies were tested by immediate saline spin, 37degrees C albumin and antiglobulin test for 2,008 transfusion candidates and 1,047 pregnant women at Severance hospital using commercial screening and identification cells (Dade, U.S.A.). RESULTS: The irregular antibodies were detected in 38 (1.24%) of total 3,055 subjects (transfusion candidates: 0.9%, pregnant women: 1.91%) . In transfusion candidates, the detected antibodies were Lewis antibodies, cold antibodies (anti-M, anti-P 1), Rh antibodies and unspeified warm antibodies, and their distributions were 56%, 22%, 17%, and 5%, respectively. In pregnant women, the detected antibodies were Lewis antibodies, Rh antibodies, anti-Jra, and unspeified warm antibodies, and their distributions were 45%, 45%, 5% and 5%, respectively. At immediate saline phase, 58% of irregular antibodies were detected. At 37degrees C albumin phase, 90% of irregular antibodies were detected and only 10% of irregular antibodies were detected at antiglobulin phase. CONCLUSIONS: Although the prevalence rates of clinically important irregular antibodies were low, 1/1000 of irregular antibodies could not be detected. Therefore, irregular antibody screening should be performed in all pretransfusion test. And, if antibody detection tests are negative, immediate saline crossmaching methods are acceptable in Korea.
Antibodies*
;
Blood Transfusion
;
Coombs Test
;
Female
;
Humans
;
Korea*
;
Mass Screening
;
Pregnant Women*
;
Prevalence*
10.The Usefulness of Fetal Fibronectin in Pregnant Women.
Kyung Mi CHOI ; Dong Hee CHO ; Samuel Y LEE
Korean Journal of Clinical Pathology 1997;17(6):968-974
BACKGROUND: The purpose of our study is to evaluate the usefulness of cervicovaginal fetal fibronectin assay for the prediction of rupture of membrane and preterm labor. METHODS: A group of 39 pregnant women was involved in this prospective study. Out of 139 pregnant women, 96 were clinically diagnosed as ruptured membranes (group A). The remaining 43 of 139 pregnant women were clinically diagnosed as preterm labor(group B). The assay was performed by using the ROMCheckTM kit (Adeza Biomedical Corp., Sunnyvale, CA). RESULTS: In group 4, fetal fibronectin (fFN) positive rate is 55% (53 patients) and negative rate is 45% (43 patients). In group B, fFN positive rate is 56% (24 patients) and negative rate is 44% (19 patients). Both group of fFN positive patients show a significantly shorter interval from sampling to delivery than fFN negative patients. Also in group A, the percentage of fFN positive patients who delivered at less than 48 hours after sampling is greater than those with fFN negative patients and in group B, the preterm delivery rate is 79% with positive fFN and 37% with negative fFN. As a predictor for preterm delivery, the presence of fFN has the sensitivity 79%, the specificity 84%, the positive Predictive value 76% and the negative predictive value 86%. CONCLUSIONS: The result suggests that a positive fFN in pregnant women who have uterine contractions and ruptured membrane indicates a significant risk for preterm delivery and labor onset, and a negative fFN is a reassuring sign.
Female
;
Fibronectins*
;
Humans
;
Labor Onset
;
Membranes
;
Obstetric Labor, Premature
;
Pregnancy
;
Pregnant Women*
;
Prospective Studies
;
Rupture
;
Sensitivity and Specificity
;
Uterine Contraction