1.Studies on the Radiation Induced Apoptosis by Morphological and Biochemical Analysis in A431 Cells.
Ji Yeul KIM ; Hee Seung BOM ; Keun Hee CHOI
Korean Journal of Nuclear Medicine 1999;33(3):306-315
PURPOSE: We performed this study to evaluate the process of radiation induced apoptosis in A431 skin epithelial cancer cell line. MATERIALS AND METHODS: Low to high dose radiation (0, 2, 5, 10, 25 Gy) was given to A431 cells by Cs-137 cell irradiator. Apoptosis was evaluated by cell morphology, dye exclusion test, and DNA laddering. RESULTS: Cell viability decreased as the radiation dose increased. Number of apoptotic bodies increased as radiation dose increased. It increased most significantly at 12 hours after irradiation. Lactate dehydrogenase activity in culture medium increased according to radiation dose and time after irradiation. CONCLUSION:: Radiation-induced apoptosis which was the main course of cell death in A431 cells could be analyzed quantitatively by counting apoptotic bodies under microscope. Apoptosis increased as radiation dose increased.
Apoptosis*
;
Cell Death
;
Cell Line
;
Cell Survival
;
DNA
;
L-Lactate Dehydrogenase
;
Skin
2.Clinical Studies on the Hyaline Membrane Disease.
Jung Hee LEE ; Gyoung Hee KIM ; Keun LEE
Journal of the Korean Pediatric Society 1984;27(9):859-864
No abstract available.
Humans
;
Hyalin*
;
Hyaline Membrane Disease*
;
Infant, Newborn
3.Relationship between Radiation Induced Activation of DNA Repair Genes and Radiation Induced Apoptosis in Human Cell Line A431.
Hee Seung BOM ; Jung Jun MIN ; Keun Hee CHOI ; Kyung Keun KIM
Korean Journal of Nuclear Medicine 2000;34(2):144-153
PURPOSE: The purpose of this study was to evaluate the relationship between radiation-induced activation of DNA repair genes and radiation induced apoptosis in A431 cell line. MATERALS AND METHODS: Five and 25 Gys of gamma radiation were given to A431 cells by a Cs-137 cell irradiator. Apoptosis was evaluated by flow cytometry using annexin V-fluorescein isothiocyanate and propidium iodide staining. The expression of DNA repair genes was evaluated by both Northern and Western blot analyses. RESULTS: The number of apoptotic cells increased with the increased radiation dose. It increased most significantly at 12 hours after irradiation. Expression of p53, p21, and hRAD50 reached the highest level at 12 hours after 5 Gy irradiation. In response to 25 Gy irradiation, hRAD50 and p21 were expressed maximally at 12 hours, but p53 and GADD45 genes showed the highest expression level after 12 hours. CONCLUSION: Induction of apoptosis and DNA repair by ionizing radiation were closely correlated. The peak time of inducing apoptosis and DNA repair was 12 hours in this study model. hRAD50, a recently discovered DNA repair gene, was also associated with radiation-induced apoptosis.
Apoptosis*
;
Blotting, Western
;
Cell Line*
;
DNA Repair*
;
DNA*
;
Flow Cytometry
;
Gamma Rays
;
Humans*
;
Propidium
;
Radiation, Ionizing
4.Meniscus Repair using Meniscus Arrow: Preliminary Report.
Kyung Taek KIM ; Sung Keun SOHN ; Dal Hee KIM
Journal of the Korean Knee Society 1998;10(1):73-77
No abstract available.
8.Allogeneic bone grafting:review of pathologic basis.
Kue Hee KIM ; In Woong UM ; Dong Keun LEE
Journal of the Korean Association of Oral and Maxillofacial Surgeons 1993;19(3):288-300
No abstract available.
9.Comparison of Transcutaneous Oxygeon Tension with Arterial Oxygeon Tension in Newborn Infants.
Jung Hee LEE ; Moon Ja KIM ; Keun LEE
Journal of the Korean Pediatric Society 1985;28(3):211-216
No abstract available.
Humans
;
Infant, Newborn*
Result Analysis
Print
Save
E-mail