No abstract available.
Animals ; Rats* ; Urinary Bladder Neck Obstruction* ; Urinary Bladder*

Closed medullary nailing has become increasingly popular in the management of fractures of the femur because of a high rate of union, a low rate of complications, and excellent return of function. But it requires good facility and excellent surgical skill. The authors experienced two cases of iatrogenic femur neck fracture during closed medullary nailing of femur shaft fracture.
Femoral Neck Fractures ; Femur Neck ; Femur

HIV-1 tat, a strong transactivator, is essential for the HIV-1 replication and AIDS progression. The Tat function is markedly inhibited by human anti-oncogene p53. This work was initiated to identify the p53-associated inhibitory mechanism on tat-mediated transactivation. Inhibitory function of P53 was confirmed by co-transfection of tat-expressing Jurkat cells with LTR-CAT plasmid, or H3Tl cells (LTR-CAT integrated HeLa cells) with different ratio of pSV-tat/pCDNA-p53 plasmids. Results from the direct protein-protein .interaction between soluble p53 and tat, and yeast two-hybrid experiments showed that the co-suppression mechanism is unlikely to be due to the direct interaction. CAT activity was not affected by tat in Jurkat cells which were transfected with p53-promoter-CAT or p53-enhancer-CAT, suggesting that the tat-mediated p53 suppression is not directly associated with p53-promoter. Finally, we have tested protein kinase activity in p53-tranfected Jurkat cells, which might phosphorylate HIV-1 tat, resulting in inhibition of tat function. Some of our data lead us to assume that the p53-mediated tat inhibition is likely to be associated with p53-associated, signaling-mediated phosphorylation of tat, resulting in the dysfunction of tat. This study is now under investigation.
Animals ; Cats ; Genes, Tumor Suppressor ; HIV-1* ; Humans ; Jurkat Cells ; Phosphorylation ; Plasmids ; Protein Kinases ; RNA Interference ; Trans-Activators ; Transcriptional Activation ; Yeasts

OBJECTIVES: To test the hypothesis that the endometrial stromal cells from patients with endometriosis responds differently to the insulin-like growth factor (IGF) compared with those from patients without endometriosis. METHODS: IGFs in peritoneal fluid (PF) from patients with endometriosis(n=18) and without endometriosis(n=12;control patients) were measured by radioimmunoassay. Endometrial stromal cells from patients with endometriosis and control patients were cultured in serum free media(SFM) in the presence or absence of PF or IGF-I(0.25-25 ng/ml) or IGF-II(5-50 ng/ml) and the proliferation of endometrial stromal cells were evaluated by [3H] thymidine incorporation test. All statistics were performed by ANOVA test and student's t-test. RESULTS: When added to SFM, IGF-I(1-25 ng/ml) increased thymidine incorporation in both endometrial stromal cells from patients with endometriosis and control patients in dose dependent manner and IGF-II(5-25 ng/ml) gave similar response in latter cells but not in former cells. Within low IGF-I(less than 100 ng/ml) PF group or high IGF-I(more than 100ng/ml) PF group, the type of endometrial stromal cells did not result in any difference in thymidine incorporation. However, regardless of the source of stromal cells, high IGF-I PF group produced a greater extent of thymidine incorporation than low IGF-I PF group in patients with endometriosis but not in control patients. Also, thymidine incorporation was higher in high IGF-I PF group of former patients than in the same group of latter patients. PF induced higher thymidine incorporation in endometrial stromal cells than the same levels(0.25-2.5 ng/ml) of IGF-I directly added to SFM. CONCLUSIONS: The effects of IGF-I in PF on endometrial stromal cells are similar regardless of their source and IGF-I is one of several growth factors that may participate in the growth of endometrial stromal cells in pelvic endometriosis.
Ascitic Fluid ; DNA ; Endometriosis* ; Female ; Humans ; Insulin-Like Growth Factor I ; Intercellular Signaling Peptides and Proteins ; Radioimmunoassay ; Somatomedins* ; Stromal Cells* ; Thymidine

Limb salvage operations for osteosarcoma of the extremity usually consist of wide excision and skeletal reconstruction. Most osteosarcoma patients are anemic prior to the surgery as majority of them undergo preoperative neo-adjuvant chemotherapy; thus, it is necessary to treat anemia before and after the surgery since limb salvage operation tends to accompany significant blood loss. Despite the fact that blood transfusion has bad influence on prognosis, complication, and postoperative outcome of cancer patients, it is still considered as a standard management to fix anemia for limb salvage operations. We would like to present a case report in which the authors succeeded in performing limb salvage operations on patients with distal femur osteosarcoma without transfusion.
Anemia ; Blood Transfusion* ; Drug Therapy ; Extremities ; Femur* ; Humans ; Limb Salvage* ; Osteosarcoma* ; Prognosis

No abstract available.
Neurofibroma* ; Scalp*

No abstract available.
Dermatitis, Contact*

No abstract available.
Humans ; Inpatients* ; Pediatrics ; Retrospective Studies*

No abstract available.
Hyperplasia ; Scrotum

PURPOSE: To evaluate the efficacy of the tension-free vaginal tape (TVT) procedure in patients with recurrent stress urinary incontinence. Therefore, the effectiveness of the procedure in patients whom previous traditional surgical procedures had failed was retrospectively reviewed. MATERIALS AND METHODS: A total of 48 women, in whom a previous traditional surgical procedure had failed, received the TVT procedure (Group I). A further 124 women received the TVT procedure as the primary anti-incontinence surgical procedure (Group II) over the same time period, who were followed up for at least 1 year after the TVT procedure, were also included in the study. The preoperative evaluations included a symptom questionnaire, physical examination and urodynamic evaluation. Uroflowmetry, postvoiding residual urine and the symptom questionnaire were evaluated postoperatively, and the pre- and postoperative results compared between the two groups. The surgical results were classified into 3 categories; cured, improved and failed. RESULTS: According to the protocol, 40 (83.3%) and 106 (85.5%) patients in Groups I and II were cured. There were no significant differences in the cure rates between the two groups. Thirty four and 58 intrinsic sphincter deficiency patients were noted in Groups I and II, respectively; of these, 76.5 and 79.3% of the patients in groups I and II were cured of their incontinence. There were no significant differences in the incidences of intra- or postoperative complications, postoperative voiding problems and patient satisfactions between the two groups. CONCLUSIONS: There were no differences in the results of the TVT procedure in women with recurrent stress urinary incontinence or who had received the procedure as the primary anti-incontinence surgical treatment. These results imply the TVT procedure can be performed as the primary choice in patients having failed previous anti-incontinence surgery.
Female ; Humans ; Incidence ; Physical Examination ; Postoperative Complications ; Surveys and Questionnaires ; Reoperation ; Retrospective Studies ; Suburethral Slings* ; Urinary Incontinence* ; Urodynamics