No abstract available.
Arthrography* ; Wrist*

Three case of plexiform schwannoma displayed multinodular masses and microscopically a multicentric pattern of growth featuring Antoni A cellular component, Verocay bodies and presence of Antoni B areas. Clinically von Recklinghausen's disease was not observed in all cases. The first patient was a 17 year old male who had a protruding nodule of walnut size which was located at the dermis of the left flank for 13 years. The second case, a 25 year old male, had an irregular whitish brown multinodular mass in the choana for 5 years. The last case, a 56 year old woman, had an ovoid yellowish brown mass with multiple nodules in the retroperitoneum.
Female ; Male ; Humans

No abstract available.
Animals ; Cryopreservation* ; Embryonic Structures* ; Mice* ; Ovum*

Ameloblastoma is a histologically benign tumour originating from epithelial components of the embryonic tooth, arrested developmentally prior to enamel formation. Clinically this tumour is locally invasive, potentially lethal and occasionally shows malignant features with systemic metastases. The maxilla is by far less frequently affected than the mandible. We have experienced a case of multicystic ameloblastoma originating from right maxilla. The patient was 39-year-old male who complained pain and numbness on right cheek. The patient was treated with subtotal maxillectomy by midfacial degloving approach. The final histopathologic diagnosis was a acanthomatous ameloblastoma.
Adult ; Ameloblastoma* ; Cheek ; Dental Enamel ; Diagnosis ; Humans ; Hypesthesia ; Male ; Mandible ; Maxilla* ; Neoplasm Metastasis ; Tooth

Green nail syndrome is characterized by greenish discoloration of the nail. It is caused by Pseudomas aeruginosa which is an aerobic gram-negative rod found in moist environment. The most common predisposing factors are frequent exposure to water and trauma history. Herein, we report two cases of green nail syndrome who developed greenish discoloration of finger nails, which were treated by systemic levofloxacin and gentian violet application.
Causality ; Fingers ; Gentian Violet ; Levofloxacin ; Pseudomonas aeruginosa ; Water

Actinic granuloma develops in the chronically sun-damaged skin of the neck, face, upper chest or arms. Lesions present as skin colored to erythematous papules and plaques that coalesce to form centrifugally enlarging annular patterns. Histologically, elastolytic granuloma is formed by a dense infiltrate of giant cells and histiocytes with active phagocytosis of elastoclastic fibers on the background of solar elastosis in the upper dermis. We report a clinically rare presentation of actinic granuloma, limited to both hands of a 75-year-old female with colon cancer.
Actins ; Aged ; Arm ; Colon ; Colonic Neoplasms ; Dermis ; Female ; Giant Cells ; Granuloma ; Hand ; Histiocytes ; Humans ; Neck ; Phagocytosis ; Skin ; Thorax

Desmoplastic trichoepithelioma is a rare, benign, adnexal tumor of hair follicle origin and is most commonly found on the face of young females. Clinically it presents as a solitary, asymptomatic, firm and annular plaque with a raised border. The histopathological findings show narrow strands of basaloid tumor cells with variable nests in a trabecular pattern and horn cysts in the desmoplastic stroma. This closely resembles morphea type basal cell carcinoma and microcystic adnexal carcinoma. We herein report a case of desmoplastic trichoepithelioma on the cheek of a 22-year-old man, with discussion of the significance of the immunohistochemical stains in the differential diagnosis of similar skin tumors, using antibodies for CD10, CD34, CEA, CK14, CK7 and Bcl-2.
Animals ; Antibodies ; Carcinoma, Basal Cell ; Cheek ; Coloring Agents ; Diagnosis, Differential ; Female ; Hair Follicle ; Horns ; Humans ; Scleroderma, Localized ; Skin ; Young Adult

Iressa(R) (ZD 1839, gefitinib) is a new anti-cancer agent which selectively inhibits the epidermal growth factor receptor (EGFR) tyrosine kinase in the pathway of the signal transduction. This agent can induce adverse effects in the cutaneous which are related to the interruption of normal epidermal cell kinetics. We report a case of paronychia in a 65-year-old man, developed in both sides of his finger and toe nails during treatment of non-small cell lung cancer (Stage IV) with Iressa(R) for 7 days. The patient came to our clinic with painful periungal inflammation with granulation tissue formation. The lesion was improved after treatment with topical or systemic antibiotics, Burrow's solution (0.3% aluminum acetate) soaking and electrodessication.
Aged ; Aluminum ; Anti-Bacterial Agents ; Carcinoma, Non-Small-Cell Lung ; Fingers ; Granulation Tissue ; Humans ; Inflammation ; Kinetics ; Lung ; Lung Neoplasms ; Nails ; Paronychia ; Porphyrins ; Protein-Tyrosine Kinases ; Receptor, Epidermal Growth Factor ; Signal Transduction ; Toes

BACKGROUND: Malassezia yeasts are lipophilic fungi that are found in 75~80% of healthy adults. Recently, various molecular biological techniques are being preferred to identify and classify the Malassezia yeasts. Pyrosequencing is a real-time DNA sequencing technique. This technology has the potential advantage of accuracy, ease-of-use, high flexibility and is now emerging as a popular platform for microbial typing. OBJECTIVE: We sought to implement novel molecular biology technique, namely pyrosequencing method in identifying and classifying Malassezia yeasts, and assess its clinical applicability. METHODS: We obtained ribosomal RNA sequences of 11 Malassezia standard strains from NCBI database. Primers for the initial PCR amplification of the target region (ITS2) and sequencing primers within the regions amplified by the PCR primers were designed using Pyrosequencing Assay Design Software (Biotage AB, Uppsala, Sweden). We obtained PCR amplifying fragments of genomic DNA isolated from the Malassezia yeasts. And pyrosequence reactions were performed using reagents provided with the PSQ 96 Sample Preparation kit. RESULTS: In the PCR analysis, all of 11 standard strains are shown at the 130 bp levels. In the pyrosequencing analysis, M. obtusa and M. furfur sequences were corresponded among 11 Malassezia standard strains. But, in 4 cases, Malassezia strains mismatched with expected Malassezia strain and in rest of 5 Malassezia strains, pyrosequencing was failed. CONCLUSION: As evidenced above, pyrosequencing analysis could provide a sensitive and rapid identification system for Malassezia species. But it still has many limitation to be applied to epidemiological surveys and clinical practice.
Adult ; Classification* ; DNA ; Fungi ; Humans ; Indicators and Reagents ; Malassezia* ; Molecular Biology ; Pliability ; Polymerase Chain Reaction ; RNA, Ribosomal ; Sequence Analysis, DNA ; Yeasts*

BACKGROUND: Malassezia yeasts are lipophilic fungi that are found in 75~80% of healthy adults. The yeasts are known to be associated with pityriasis versicolor, seborrheic dermatitis, Malassezia folliculitis, and recently its pathogenicity is being expanded to other various skin disorders, such as atopic dermatitis and acne vulgaris. Recently, various molecular biological techniques are being preferred over morphological analysis. In order to perform a DNA-based diagnostic test, availability of a simple, rapid, and reliable DNA extraction protocol is essential. OBJECTIVE: We sought to implement novel molecular biology technique, namely colony PCR method using microwave as the easiest way to amplification of Malassezia target DNA, and assess its clinical applicability. METHODS: Instead of using templates of purified genomic DNA, we performed the PCR directly from Malassezia colonies. A fresh yeast colony transferred to the bottom of a microcentrifuge tube and microwaved for 1 min three times in the presence of a pyrex beaker containing 50 ml of sterile water to dissipate excess heat. Following this microwave lysis, PCR-reaction mixture was added directly to the microcentrifuge tube. Two DNA extraction methods (boiling method, glass beads method) were used for comparing the sensitivity and effectiveness with the colony PCR method. All reactions were performed using the primers 26S and ITS1 complementary to the rDNA region. Results 1. As a result of gel electrophoresis, we recognized expected PCR products (approximately 580 bp for 26S rDNA and 250~320 bp for ITS1) from both colony PCR method and two DNA extraction methods (boiling method, glass beads method). 2. As a result of measuring nucleic acid level with the spectrophotometer, colony PCR disregarding DNA extraction process shows relatively similar PCR efficacy compared with the boiling and glass beads method. And there is no significant difference among those methods statistically (p>0.001). 3. In conducting the PCR method, boiling method required approximately 400 minutes, and glass beads method required approximately 360 minutes, respectively. As contrasted with two methods, colony PCR method required approximately 150 minutes, and could be capable of saving time. In addithion, colony PCR had an economic efficiency comparing with boiling method and glass beads methods. CONCLUSIONS: All these findings suggest that directly application of the Malassezia yeasts obtained from culture colony for PCR reaction is a fast, reliable, cost-effective and simple method for performing any PCR-based protocol including diagnostic tests.
Acne Vulgaris ; Adult ; Dermatitis, Atopic ; Dermatitis, Seborrheic ; Diagnostic Tests, Routine ; DNA ; DNA, Ribosomal ; Electrophoresis ; Folliculitis ; Fungi ; Glass ; Hot Temperature ; Humans ; Malassezia* ; Microwaves ; Molecular Biology ; Polymerase Chain Reaction* ; Skin ; Tinea Versicolor ; Virulence ; Water ; Yeasts*